To learn more about the fungus' tolerance for salt, Tami Kis Papo at the University of Haifa grew samples in liquid and solid media at salinities from zero up to 90 percent of Dead Sea water. The researchers found that it had viable spores when grown in 70 percent diluted Dead Sea water, conditions equivalent to an algal bloom in the Dead Sea 20 years ago. A study conducted by Alfons R. Weig at the University of Bayreuth of E. rubrum's transcriptome, that small fraction of the genome that encodes the RNA molecules in order to carry out instructions to build and maintain cells, showed that in high salinity conditions, the fungal cells need to keep cell membrane transport under tight control. "This clearly indicates that the fungus tries to cope 'actively' with its extreme environment and does not simply fall into dormancy," the team noted, "as might be expected by the greatly reduced growth rates."
In addition to contributing to a better understanding of salt tolerance mechanisms for agriculture, this work may also have applicability to the DOE's interests in developing new strategies to improve biofuels production. For instance, the DOE JGI and its partners are sourcing microbial and fungal enzymes for more effective biomass pretreatment with ionic liquids, environmentally benign organic salts often used as green chemistry substitutes for volatile organic solvents.
|Contact: David Gilbert|
DOE/Joint Genome Institute