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Salt-loving microbe provides new enzymes for the production of next-gen biofuels
Date:6/30/2011

at the Great Salt Lake and sequenced at the DOE JGI as part of the Genomic Encyclopedia of Bacteria and Archaea (GEBA) project.

"This is one of the only reports of salt-tolerant cellulases, and the only one that represents a true 'genome-to-function' relevant to ionic liquids from a halophilic environment," said Simmons of the study published June 30, 2011 in Green Chemistry. "This strategy enhances the possibility of identifying true obligatory halophilic enzymes." Such salt-tolerant enzymes, particularly cellulases, offer significant advantages for industrial utility over conventional enzymes.

In collaboration with Jerry Eichler from Ben Gurion University of the Negev in Israel they cloned and expressed a gene from H. utahensis in another haloarchaeal microbe, and were able to identify a salt-dependent enzyme that can tolerate high temperatures and is resistant to ionic liquids. "This project has established a very important link between genomic science and the realization of enzymes that can handle very demanding chemical environments, such as those present in a biorefinery," said Simmons.

The group plans to expand this research to develop a full complement of enzymes that is tailored for the ionic liquid process technology with the goal of demonstrating a complete biomass-to-sugar process, one they hope can enable the commercial viability of advanced biofuels.


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Contact: David Gilbert
925-296-5643
DOE/Joint Genome Institute
Source:Eurekalert  

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