Navigation Links
Salmonella infection, but not as we know it
Date:4/25/2012

BBSRC-funded researchers at Cambridge University have shed new light on a common food poisoning bug. Using real-time video microscopy, coupled with mathematical modelling, they have changed our assumptions about Salmonella and how it infects human cells. The research was published in Interface.

Salmonella is an important bacterium to study as it causes a range of diseases in humans and animals. It is capable of growing and reproducing inside macrophages - a type of white blood cell that ingests foreign material - ultimately destroying them. These macrophage cells are key players in the immune response to invaders and so the control of Salmonella within these cells is critical to surviving an infection. However, fundamentally important factors in infection events - such as the rate at which Salmonella infects cells, how frequently this occurs and the probability of infection - had not previously been calculated because it was thought impossible to do so.

Dr Bryant, from the University of Cambridge, said: "Understanding how these bacteria invade, survive, proliferate and kill vital macrophage cells provides a wealth of knowledge to help improve our health. For the first time, we have been able to calculate the rate at which Salmonella can infect macrophages and we have also seen evidence of dual infection and reinfection of a single cell."

Instead of relying on figures from large populations of infected cells, such as changes in total bacterial number over time, finer measurements of the individual steps of infection were considered. The researchers used two independent approaches for their calculations: mathematical modelling of Salmonella infection experiments, and analysis of real-time video microscopy of individual infection events.

Their research found that many incorrect assumptions had been made about Salmonella infection, particularly that macrophages are highly susceptible to infection. Their data showed that infection occurrences after initial contact between a bacterium and macrophage were low. The probability of that bacterium infecting the cell is less than 5 per cent. However, they also showed that an infected macrophage can be reinfected by a second bacterium. The concept of reinfection by Salmonella had not been considered before and this previously overlooked mechanism may make an important contribution to total bacterial numbers in infection studies.

The study also highlighted the fact that some cells are far more susceptible to infection than others. Rather than grouping all macrophages together in terms of their susceptibility to infection, the research shows that there is a spectrum of susceptibility.

"Our research revealed novel biological processes that occur when Salmonella interacts with macrophages. It will lead to a reconsideration of the mechanisms behind infection which will be important for the future development of intervention strategies," added Dr Bryant.


'/>"/>
Contact: Rob Dawson
rob.dawson@bbsrc.ac.uk
01-793-413-204
Biotechnology and Biological Sciences Research Council
Source:Eurekalert

Related biology news :

1. UCSB researchers discover particularly dangerous Salmonella
2. UCSB researchers find a way to detect stealthy, hypervirulent Salmonella strains
3. UCI-led study uncovers how Salmonella avoids the bodys immune response
4. How Salmonella forms evil twins to evade the bodys defenses
5. Newfound hijacked proteins linked to salmonella virulence
6. E. coli, salmonella may lurk in unwashable places in produce
7. Salmonella stays deadly with a beta version of cell behavior
8. New target found for nitric oxides attack on salmonella bacteria
9. Bacterium Salmonella enterica regulates virulence according to iron levels found in its surroundings
10. Salmonella utilize multiple modes of infection
11. Zooming in on the weapons of Salmonella
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/23/2017)... , Mar. 23, 2017 Research and Markets ... Market Analysis & Trends - Industry Forecast to 2025" report ... ... at a CAGR of around 8.8% over the next decade to ... report analyzes the market estimates and forecasts for all the given ...
(Date:3/20/2017)... PMD Healthcare announces the release of its ... System (WMS), a remote, real-time lung health monitoring and ... is a Medical Device, Digital Health, and Chronic Care ... innovative solutions that empower people to improve their healthcare ... developed the first ever personal spirometer, Spiro PD, which ...
(Date:3/7/2017)... 7, 2017 Brandwatch , the leading social intelligence ... Prince,s Trust to uncover insights to support its reporting, help ... The UK,s leading youth charity will be using Brandwatch Analytics ... get a better understanding of the topics and issues that are ... ...
Breaking Biology News(10 mins):
(Date:3/28/2017)... ... ... Franz Inc ., the leading supplier of Semantic Graph Database technology, ... by Bloor Research in its recent Graph Database Market Update report. ... Gruff, it was rated as the easiest product to use.” – Bloor Research , ...
(Date:3/28/2017)... WASHINGTON , March 28, 2017 /PRNewswire/ ... launch single-cell sequencing during the American Association for Cancer ... Convention Center in Washington, D.C. , ... differential gene expression of thousands of cells at the ... Experts on-hand at AACR to discuss ...
(Date:3/28/2017)... HOLLISTON, Mass. , March 28, 2017 ... "Company"), a biotechnology company developing bioengineered organ implants to ... bronchus and trachea, today announced that Jim McGorry, ... 3D Printing and BioEngineering panel at the ... 2017 at 2:30 PM ET in Cambrige, Massachussetts. The ...
(Date:3/28/2017)... -RepliCel Life Sciences Inc. (OTCQB: REPCF) (TSXV: RP) (FRA:P6P2) ("RepliCel" ... clinical data from its phase 1/2 tendon repair study investigating ... (RCT-01) as a treatment for Achilles tendinosis. ... The clinical trial ... 6 months and showed no serious adverse events related to ...
Breaking Biology Technology: