Navigation Links
Salk scientists crack riddle of important drug target
Date:12/2/2013

n this artificial amino acid, it grabs nearby molecules," explains Irene Coin, a postdoctoral fellow in Wang's team. "It's like a sticky hand."

When the artificial amino acid, Azi, was added to any spot where the CFR1R ligand attached to the receptor, the sticky hand grabbed the ligand, a molecule called urocortin-1, and kept it bound to the receptor. If Azi was integrated into a place where urocortin-1 didn't associate, however, it would have nothing to grab. By detecting whether CFR1R and urocortin-1 become irreversibly attached, the researchers would know whether the Azi had been integrated into part of the binding pocket or not.

Repeating this technique throughout the CFR1R molecule revealed that the receptor's binding pocket consisted of at least 35 amino acids. But that didn't give the researchers the full picture they wanted of the interaction.

"This first, sticky hand probe had given us information about the shape of the binding pocket," says Wang. "But we still didn't know how the ligand is oriented inside that pocket."

So they used a second probe--one which was more selective than the "sticky hand" in the receptor. This time, the probe would only capture one particular amino acid--cysteine. "We inserted cysteines along the ligand to figure out which parts of the receptor were close to precise spots of the ligand", says Wang. It took more than a hundred different combinations to get a perfect match, where the artificial amino acids in the receptor lined up with the cysteines in the urocortin-1.

"We discovered that the ligand lies in the receptor's binding pocket like a too-tall person in a bathtub," Coin says. "One end of the ligand is like the head sticking out, and on the other end, the feet are dangling out."

"This shape makes sense in light of previous data," says Wang. "Because we already knew that you can add a lot of molecules onto the feet of the ligand and it doesn't affect the receptor-li
'/>"/>

Contact: kat Kearney
kkearney@salk.edu
858-453-4100
Salk Institute
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Stanford scientists develop gene therapy approach to grow blood vessels in ischemic limbs
2. Queens scientists seek vaccine for Pseudomonas infection
3. Scientists produce eye structures from human blood-derived stem cells
4. American Society of Plant Biologists honors early career women scientists
5. Brandeis scientists win prestigious prize for circadian rhythms research
6. Scientists discover new method of proton transfer
7. Salk scientists open new window into how cancers override cellular growth controls
8. WileyChina.com - Now Featuring Bespoke Pages for China’s Life Scientists
9. Scientists win $2 million to study new pathway in development and maintenance of lymphoma
10. UGA scientists reveal genetic mutation depicted in van Goghs sunflower paintings
11. Genetic mutation depicted in van Goghs sunflower paintings revealed by scientists
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Salk scientists crack riddle of important drug target
(Date:9/16/2014)... Three years after the Hesperides vessel returned to Spain ... researchers have an increasingly clear picture of how the ... Specifically, the input of pollutants from the atmosphere is ... the most remote areas of the planet, and it ... findings are presented this week in CSIC Residence for ...
(Date:9/16/2014)... a peacock show its extravagant, colorful tail feathers in ... have used a special camera developed by an engineer ... female northern swordtail fish choose their mates based on ... of Texas at Austin used a bioinspired polarization camera ... science & engineering at Washington University, to make the ...
(Date:9/16/2014)... the salmon-rich waters of Southeast Alaska to the white ... lobster, lumber and tourist towns, coastal residents around the ... environments divide along political lines. That,s a primary finding ... sociologists published this month in the journal Society ... of environment-related differences from place to place to place. ...
Breaking Biology News(10 mins):The Malaspina Expedition confirms that pollution reaches even the most remote areas of the ocean 2The Malaspina Expedition confirms that pollution reaches even the most remote areas of the ocean 3Camera developed at WUSTL sheds light on mate choice of swordtail fish 2Politics divide coastal residents' views of environment, UNH research finds 2Politics divide coastal residents' views of environment, UNH research finds 3
... MADISON A discovery at the University of Wisconsin-Madison ... create a "hydrogen economy" that would use this abundant ... hydrogen is the ultimate non-carbon, non-polluting fuel for storing ... for energy, hydrogen produces water but no carbon dioxide. ...
... the bacteria that form the base of the ocean,s ... a new study. In climate change, as in ... dioxide levels and temperature rise globally, scientists increasingly want ... perish in the environment of tomorrow. The answer ...
... Brookhaven National Laboratory have identified two promising candidates for ... of ailments ranging from colds to gastrointestinal disorders to ... , a journal of the Federation of European Biochemical ... compounds to determine which might block the effects of ...
Cached Biology News:New catalyst could cut cost of making hydrogen fuel 2Greenhouse gas likely altering ocean foodchain 2Scientists identify promising antiviral compounds 2Scientists identify promising antiviral compounds 3
(Date:9/16/2014)... -- This report analyzes the worldwide markets for Bioinformatics in ... Biocontent. The report provides separate comprehensive analytics for the US, ... Europe , Asia-Pacific , ... Annual estimates and forecasts are provided for the period 2013 ... these markets. Market data and analytics are derived from primary ...
(Date:9/16/2014)... University scientists who created a deicing film for radar ... a transparent coating for glass. , The new ... could keep glass surfaces from windshields to skyscrapers free ... radio frequencies (RF). , The technology was introduced ... Applied Materials and Interfaces . , The material ...
(Date:9/16/2014)... , Sept. 16, 2014  The Council ... entrepreneurial support organization in the country, today released ... Report . It showed that ... North Carolina startups in the technology, ... more global investment from a variety of sources. Significant ...
(Date:9/16/2014)... BARBARA, Calif. , Sept. 16, 2014 /PRNewswire-iReach/ ... Strength index (BMSi) test identifies patients with bone ... cannot.  Over 160 million people worldwide are at ... detected by today,s standard bone strength test, the ... the 2014 American Society for Bone and Mineral ...
Breaking Biology Technology:Global Bioinformatics Industry 2Global Bioinformatics Industry 3Global Bioinformatics Industry 4Global Bioinformatics Industry 5Global Bioinformatics Industry 6Global Bioinformatics Industry 7Global Bioinformatics Industry 8Global Bioinformatics Industry 9Global Bioinformatics Industry 10Global Bioinformatics Industry 11Global Bioinformatics Industry 12Global Bioinformatics Industry 13Global Bioinformatics Industry 14Global Bioinformatics Industry 15Global Bioinformatics Industry 16Global Bioinformatics Industry 17Global Bioinformatics Industry 18Global Bioinformatics Industry 19Global Bioinformatics Industry 20Global Bioinformatics Industry 21Global Bioinformatics Industry 22Global Bioinformatics Industry 23Nanoribbon film keeps glass ice-free 2CED's Mid-Year Update to its Innovators Report Shows NC Startups Attracting Investment From Across the Globe with Several High-Profile Investment Deals, IPOs and Acquisitions Signaling Strength of Entrepreneurial Sector 2CED's Mid-Year Update to its Innovators Report Shows NC Startups Attracting Investment From Across the Globe with Several High-Profile Investment Deals, IPOs and Acquisitions Signaling Strength of Entrepreneurial Sector 3CED's Mid-Year Update to its Innovators Report Shows NC Startups Attracting Investment From Across the Globe with Several High-Profile Investment Deals, IPOs and Acquisitions Signaling Strength of Entrepreneurial Sector 4ActiveLife's BMSi Test Provides Hope for 160 Million with Undetectable Risk of Bone Fracture 2ActiveLife's BMSi Test Provides Hope for 160 Million with Undetectable Risk of Bone Fracture 3
... of PCR reactions with Herculase enhanced DNA,polymerase ... ,Stratagene , Stratagene recently released Herculase ... that provides,superior performance in both routine and demanding ... exhibits greater fidelity than,commercial DNA polymerase blends, in ...
... ,Single-tube amplification method for high-throughput screening of HSV ... Peter Pingerelli ,Melanie Palmer Dwight DuBois ,Stratagene , ... , ff ,a single-tube ... Because,it is performed in a closed-tube system and requires ...
... developed for,highfidelity PCR , , Kirk B. Nielson ... Barbara McGowan * Holly Hogrefe ,Stratagene Cloning Systems, Inc. ... Precision PCR system,* to an expanding line of PCR ... mixture of Taq2000 and Pfu DNA polymerases and a ...
Cached Biology Technology:Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR,Performance 2Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR,Performance 3Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR,Performance 4Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR,Performance 5Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR,Performance 6Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR,Performance 7Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR,Performance 8HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex,Virus DNA 2HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex,Virus DNA 3HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex,Virus DNA 4High-Fidelity PCR with a Novel Polymerase Mixture 2High-Fidelity PCR with a Novel Polymerase Mixture 3High-Fidelity PCR with a Novel Polymerase Mixture 4High-Fidelity PCR with a Novel Polymerase Mixture 5High-Fidelity PCR with a Novel Polymerase Mixture 6High-Fidelity PCR with a Novel Polymerase Mixture 7High-Fidelity PCR with a Novel Polymerase Mixture 8High-Fidelity PCR with a Novel Polymerase Mixture 9High-Fidelity PCR with a Novel Polymerase Mixture 10High-Fidelity PCR with a Novel Polymerase Mixture 11
Recombinant Mouse Wnt-3a...
... There are at least 20 structurally ... of the TGF-beta superfamily. BMPs were originally ... bone formation. They are also involved in ... organs. BMPs regulate the growth, differentiation, chemotaxis ...
RABBIT ANTI HUMAN TRRAP (CT) Immunogen: Recombinant C-terminal kinase-related domain of human TRRAP....
RABBIT ANTI HUMAN CHYMOTRYPSIN...
Biology Products: