Navigation Links
Rong Li Lab reports protein interactions of MAP kinase signaling pathway
Date:12/13/2007

The Stowers Institutes Rong Li Lab, in collaboration with the Institutes Imaging Center, has achieved a quantitative in vivo measurement of the dynamic protein-protein interactions in the mitogen-activated protein (MAP) kinase cascade signaling pathway, which is critical to growth and differentiation decisions in all eukaryotic cells.

The paper, Mapping Dynamic Protein Interactions in the MAP Kinase Signaling Using Live-Cell Fluorescence Fluctuation Spectroscopy and Imaging, was posted to the Web site of Proceedings of the National Academy of Sciences (PNAS) yesterday and will appear in a future print issue of the journal.

In this work, Brian Slaughter, Ph.D., Postdoctoral Research Fellow; Joel Schwartz, Ph.D., Managing Director of the Imaging Center; and Rong Li, Ph.D., Investigator, used sophisticated biophysical techniques to perform quantitative biochemical measurements directly in live yeast cells.

It turns out that by using three fluorescence-based analyses we could assess the movement, concentration, and state of protein hetero- and homo-oligomerization at the single cell level, said Dr. Slaughter. It is a significant advance to be able to apply these quantitative techniques to the model system of yeast.

These technical breakthroughs represent an exciting emerging direction for molecular analysis in the future, said Dr. Li. They will enable biological systems to be understood with precise information regarding when, where, and to what extent molecules interact with each other during important regulatory processes.

The Rong Li lab worked closely with the Institutes Imaging Center to perfect the application of these techniques in yeast, calling on the Centers expertise and cutting-edge instrumentation for microscopy-based technology.

This work demonstrates the Institutes tremendous strength for live-cell quantitative analysis, said Robb Krumlauf, Ph.D., Scientific Director. I believe this and simila
'/>"/>

Contact: Marie Jennings
mfj@stowers-institute.org
816-926-4015
Stowers Institute for Medical Research
Source:Eurekalert

1

GOOD

Page: 1 2

Related biology news :

1. BIO-key Reports Second Quarter 2007 Results
2. Communication Intelligence Corporation Reports Second Quarter 2007 Financial Results
3. Emory paleontologist reports discovery of carnivorous dinosaur tracks in Australia
4. BIO-key Reports Third Quarter 2007 Results
5. WHRC releases 4 key reports
6. Protein chatter linked to cancer activation
7. Scientists link fragile X tremor/ataxia syndrome to binding protein in RNA
8. Researchers identify proteins involved in new neurodegenerative syndrome
9. Low levels of key protein may indicate pancreatic cancer risk
10. Structure of 450 million year old protein reveals evolutions steps
11. Scientists retrace evolution with first atomic structure of an ancient protein

Post Your Comments:
*Name:
*Comment:
*Email:
(Date:11/6/2009)...ov. 6 Auburn Hills, MI-based Law E... installing its locker system and patent-pending B....S. Department of Health and Human Services, Natio...MD. The locker system will support the NIH,s enti... our nation,s medical research agency. The projec...
(Date:11/5/2009)...n.Researchers at Yale University have developed sy...immune response to HIV and HIV-infected cells, as ...published online in the Journal of the American C...approaches for these diseases. , The molecules ...V" (ARM-H) and "antibody-recruiting molecule targe...
(Date:11/4/2009)... news in natural science) , United States Usin...s water today than it did 35 years ago, despite a ...use are partly attributable to alternative cooling...tion systems. According to the latest USGS water u...ited States goes to cooling thermoelectric power p...
Breaking Biology News(10 mins):New synthetic molecules trigger immune response to HIV and prostate cancer 2USGS science picks 2USGS science picks 3USGS science picks 4USGS science picks 5USGS science picks 6USGS science picks 7Risks of Delaying ACL Reconstruction in Young Athletes May Be Too High Study Shows 51401 1Risks of Delaying ACL Reconstruction in Young Athletes May Be Too High Study Shows 51401 2Risks of Delaying ACL Reconstruction in Young Athletes May Be Too High Study Shows 51401 3Risks of Delaying ACL Reconstruction in Young Athletes May Be Too High Study Shows 4713 1Risks of Delaying ACL Reconstruction in Young Athletes May Be Too High Study Shows 4713 2Federal Bureau of Investigation Awards Lockheed Martin Biometric Card Scanning Service Contract 9225 1Federal Bureau of Investigation Awards Lockheed Martin Biometric Card Scanning Service Contract 9225 2
...man (University of Wisconsin School of Medicine an...ew insight into the pathogenesis of neurodegenerat...asia (A-T) is a rare, genetic immunodeficiency dis...aracterized by neurodegeneration and cancer predis...the ataxia telangiectasia mutated (ATM) gene. , ...
...ts hard to find a better source of inspiration tha... interdisciplinary group of researchers at the U.S...ng bacteria to synthesize magnetic nanoparticles t... in magnetic inks and high-density memory devices...om-temperature synthesis of ferromagnetic nanopart...
...ar to most people, and modern conclusions usually ...lished this week in the open-access journal PLoS B...en the genes we inherit—nature—and the environment...st. As we approach the age of personal genomics, i...ic variations we carry, it is important to underst...
Other Biology News:Researchers mimic bacteria to produce magnetic nanoparticles 2Researchers mimic bacteria to produce magnetic nanoparticles 3
(Date:11/6/2009)... agenda for Arrowhead’s 2010 Personalized Me...ke place on February 22-24, 2010 in Philadelphia, ...e will highlight key issues in forming partnership...riences. , Minneapolis, M...head’s 2010 Personalized Medicine Partnersh...
(Date:11/5/2009)... 5 The endangered black-footed fer... This rare carnivore feeds on prairie dogs. The...ften entire population die-offs in towns occur. T...emented a ferret breeding program and has been rel...y native habitat. Unfortunately, plague can sweep...
(Date:11/5/2009)... Nov. 5 ThermoGenesis Co...tive products for processing and storing adult ste...ter of fiscal 2010. ,, For the quarter ended Se... $5.2 million, a 15 percent increase over revenues...The Company said the key contributors to the growt...
(Date:11/5/2009)... BioSpecifics Technologie...any developing first-in-class collagenase-based pr...the third quarter ended September 30, 2009. ,,...is year, the FDA,s Arthritis Advisory Committee un...oval of XIAFLEX(TM) for the treatment of Dupuytren...
Breaking Biology Technology:Leaders in Biopharma and Molecular Diagnostics to Discuss Key Issues and Opportunities in Forming Partnerships at 2010 Personalized Medicine Partnerships Conference 2Leaders in Biopharma and Molecular Diagnostics to Discuss Key Issues and Opportunities in Forming Partnerships at 2010 Personalized Medicine Partnerships Conference 3Colorado Companies Team Up With U.S. Fish & Wildlife Service to Protect Black-Footed Ferret 2ThermoGenesis Reports 15 Percent Increase in First Quarter Revenues; Improved Bottom Line 2ThermoGenesis Reports 15 Percent Increase in First Quarter Revenues; Improved Bottom Line 3ThermoGenesis Reports 15 Percent Increase in First Quarter Revenues; Improved Bottom Line 4ThermoGenesis Reports 15 Percent Increase in First Quarter Revenues; Improved Bottom Line 5ThermoGenesis Reports 15 Percent Increase in First Quarter Revenues; Improved Bottom Line 6ThermoGenesis Reports 15 Percent Increase in First Quarter Revenues; Improved Bottom Line 7ThermoGenesis Reports 15 Percent Increase in First Quarter Revenues; Improved Bottom Line 8BioSpecifics Technologies Corp. Reports Third Quarter 2009 Financial Results 2BioSpecifics Technologies Corp. Reports Third Quarter 2009 Financial Results 3BioSpecifics Technologies Corp. Reports Third Quarter 2009 Financial Results 4BioSpecifics Technologies Corp. Reports Third Quarter 2009 Financial Results 5
...binson has many stories to tell, but perhaps noth...vity than the story of a little girl drawing in th...or to the J. Paul Getty Trust in Los Angeles and...y and innovation, told the story during a keynote ... Medical Device Association Conference. , ,Noting...
...ll acquainted with my dislike of Cingular,s 8125 s...le 5 . I lay much of the responsibility for the po...ner, HTC. Windows Mobile 5 is what it is - a platf...e functionality to complement the PC-like applicat...d if I,d like to review the Treo 700w , I welcome...
...e dust has settled in the case of Advanced Cell T..., just what is the judgment of Wisconsin scientist....-based company? , ,The question was put to three...cell basics at the Medical College of Wisconsin ....d and suggested the company exaggerates its findin...
Other Biology Technology:Sir Ken Robinson: Companies, kids "get" innovation, but K-12 doesn't nurture it 2Sir Ken Robinson: Companies, kids "get" innovation, but K-12 doesn't nurture it 3Treo 700w solves my smart phone woes 2Treo 700w solves my smart phone woes 3Wisconsin researchers defend stem cell company 2Wisconsin researchers defend stem cell company 3
Rabbit Anti-Human ECE1 Polyclonal Antibody, Unconjugated from Atlas Antibodies
Goat Anti-Human/Mouse/Rat LAR Polyclonal Antibody, Unconjugated from R&D Systems
X-Gal from GE Healthcare, formerly Amersham Biosciences
Goat Anti-Human Jagged 2 Polyclonal Antibody, Biotin Conjugated from R&D Systems
Biology Products: