Navigation Links
Ripening secrets of the vine revealed
Date:11/20/2007

Whether you prefer a Cabernet Sauvignon or a Pinot Noir grape variety, two new research articles published in the online open access journal, BMC Genomics, offer a host of new genetic information on fruit ripening for this economically important fruit crop.

The grapevine's gene expression analysis reveals two distinct molecular and functional phases that correspond with the green and red grape stages. And researchers have reported the first biochemical evidence that reactive oxygen species accumulate during the colour transition. Stefania Pilati and fellow researchers from the IASMA Research Center, San Michele all'Adige, Italy, investigated ripening Pinot Noir grapes (Vitis vinifera L.) to identify fruit ripening genes and investigate seasonal influences. They found a core set of more than 1,400 ripening-specific genes that fluctuated similarly across three growing seasons and a smaller gene group strongly influenced by climatic conditions.

During the green berry (pre-vraison) phase, numerous genes involved in hormonal signalling and transcriptional regulation were modulated, suggesting large-scale cellular metabolism reprogramming. Auxin, ethylene and light played pivotal roles. During the following ripening (post-vraison) phase, genes for cell-wall organization and biogenesis, carbohydrate and secondary metabolisms, and stress response came into play, whereas photosynthesis was strongly repressed. These transcriptional events tally with the processes of berry softening and accumulation of sugar, colour and aroma compounds, which ultimately determine berry and wine quality. At vraison, the intervening point when grapes slow down their growth and change colour, this study highlighted an oxidative burst involving hydrogen peroxide (H2O2), and an extensive modulation of the enzymatic anti-oxidative network.

Meanwhile, Laurent G. Deluc and colleagues from the University of Nevada, Reno and the Boston University School of Medicine, USA, took a closer look at the V. vinifera Cabernet Sauvignon variety, surveying seven different stages of grape berry development. The team mapped pronounced differences throughout development in messenger-RNA (mRNA) expression for genes that play key functional roles in a host of processes. These included organic and amino acid metabolism, photosynthesis, circadian cycles and pathogen resistance.

In particular, the researchers recorded changes associated with transcription factor expression patterns, abscisic acid (ABA) biosynthesis, and calcium signalling genes that identified candidate factors likely to participate in vraison, or aroma compound production, and in pathway regulation and sequestration of flavonoid compounds. Some mRNAs were observed to decrease or increase specifically throughout ripening and sugar metabolism gene expression pattern analysis revealed an alternative and previously uncharacterised pathway for glucose and triose phosphate production invoked from vraison to mature berries.

Despite the grapevine's importance, genetic cues underlying the biochemical and physical changes during berry and flavour development have lain undiscovered - until now. "The large number of regulatory genes we have identified represents a powerful new resource for dissecting the mechanisms of fruit ripening control in non-climacteric plants", Pilati and co-workers say. Meanwhile, the second team say they have identified "a set of previously unknown genes potentially involved in critical steps associated with fruit development that can now be subjected to functional testing".


'/>"/>

Contact: Charlotte Webber
press@biomedcentral.com
44-020-763-19980
BioMed Central
Source:Eurekalert

Related biology news :

1. MIT unraveling secrets of red tide
2. Scientists eye secrets of retinal regeneration
3. NMR researchers unlock hydrogens secrets to spot polymorphism in pharmaceuticals
4. Scientists gather in New Zealand to share oceans secrets
5. New study uncovers secrets behind butterfly wing patterns
6. Eat chocolate, drink wine, add fun to life: SLU geriatrician shares secrets of staying young
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/17/2017)... , April 17, 2017 NXT-ID, Inc. ... company, announces the filing of its 2016 Annual Report on Form ... Exchange Commission. ... Form 10-K is available in the Investor Relations section of the ... on the SEC,s website at http://www.sec.gov . 2016 ...
(Date:4/11/2017)... 2017 Research and Markets has announced the ... to their offering. ... eye tracking market to grow at a CAGR of 30.37% during ... Market 2017-2021, has been prepared based on an in-depth market analysis ... and its growth prospects over the coming years. The report also ...
(Date:4/5/2017)... 5, 2017  The Allen Institute for Cell Science ... a one-of-a-kind portal and dynamic digital window into the ... the first application of deep learning to create predictive ... lines and a growing suite of powerful tools. The ... and future publicly available resources created and shared by ...
Breaking Biology News(10 mins):
(Date:10/11/2017)... (PRWEB) , ... October 11, 2017 , ... ... compared the implantation and pregnancy rates in frozen and fresh in vitro ... of progesterone and maternal age to IVF success. , After comparing the results ...
(Date:10/10/2017)... ... 2017 , ... Dr. Bob Harman, founder and CEO of VetStem Biopharma, ... The event entitled ā€œStem Cells and Their Regenerative Powers,ā€ was held on ... DVM, MPVM was joined by two human doctors: Peter B. Hanson, M.D., Chief of ...
(Date:10/9/2017)... , Oct. 9, 2017  BioTech Holdings ... mechanism by which its ProCell stem cell therapy ... limb ischemia.  The Company, demonstrated that treatment with ... of limbs saved as compared to standard bone ... molecule HGF resulted in reduction of therapeutic effect.  ...
(Date:10/7/2017)... ... October 06, 2017 , ... ... applications consulting for microscopy and surface analysis, Nanoscience Instruments is now expanding ... offers a broad range of contract analysis services for advanced applications. Services ...
Breaking Biology Technology: