Navigation Links
Rice scientists divide and conquer
Date:12/28/2009

Half a protein is better than none, and in this case, it's way better than a whole one. A Rice University lab has discovered that dividing a particular fluorescent protein and using it as a tag is handy for analyzing the workings of live cells, particularly in the way they employ iron-sulfur clusters.

Iron and sulfur in just the right amounts are critical to good health. They're in the food people eat and vitamins they take every day, but having too much or too little in the cells can cause serious problems.

Iron-sulfur clusters are molecules with as few as four atoms. They are manufactured and regulated by proteins in living cells, and their role is a fairly recent field of study for researchers interested in Friedreich's ataxia, sideroblastic anemia and myopathy, diseases caused by defects in proteins. But until now, there's been no way to look at such "metalloclusters" in living cells.

Jonathan Silberg, an assistant professor of biochemistry and cell biology at Rice, has been studying the mysteries of these molecules for years. He has come up with a way to see what they're doing in living cells. Silberg and his team published a paper in the December edition of Chemistry & Biology that details a new technique for imaging clusters that involves attaching them, through an intermediary, to fluorescent fragments of protein.

That intermediary is a human protein called GRX2, a glutaredoxin that helps cells deal with oxidative damage on other proteins. Its activity can be switched off in test tubes by association with an iron-sulfur cluster. The team had already proved that GRX2 would still bond with iron-sulfur clusters even when tagged with a green fluorescent protein; this makes it useful for in vitro studies, but the fluorescence wasn't strong enough to be seen in living cells.

However, attaching fragments of a yellow fluorescent protein called Venus to monomers (single molecules) of GRX2 worked quite well.
'/>"/>

Contact: David Ruth
druth@rice.edu
713-348-6327
Rice University
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. UK scientists working to help cut ID theft
2. Scientists show that mitochondrial DNA variants are linked to risk factors for type 2 diabetes
3. Comet probes reveal evidence of origin of life, scientists claim
4. Scientists link fragile X tremor/ataxia syndrome to binding protein in RNA
5. Male elephants get photo IDs from scientists
6. Scientists retrace evolution with first atomic structure of an ancient protein
7. Muscle mass: Scientists identify novel mode of transcriptional regulation during myogenesis
8. Carnegie Mellon scientists develop nanogels that enable controlled delivery of carbohydrate drugs
9. Clemson scientists shed light on molecules in living cells
10. Scientists tackle mystery mountain illness
11. T. rex quicker than Becks, say scientists
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/17/2014)... Mo. April 17 In the most densely forested ... forests reflect two centuries of human needs, values and ... and clearing forests for agriculture and development, have set ... a U.S. Forest Service study reports. , The report ... conditions and management needs in the Northern United States ...
(Date:4/17/2014)... 17, 2014The development of stem cell therapies to cure ... characterize stem cell populations based on cell surface markers. ... new marker that is highly expressed in a type ... which they describe in an article in BioResearch ... Liebert, Inc., publishers. The article is available free on ...
(Date:4/17/2014)... ALBUQUERQUE, N.M. A credit-card-sized anthrax detection cartridge developed ... small business makes testing safer, easier, faster and cheaper. ... causes anthrax, is commonly found in soils all over ... illness in both humans and animals. The bacteria can ... to B. anthracis may occur through skin ...
Breaking Biology News(10 mins):Five anthropogenic factors that will radically alter northern forests in 50 years 2Five anthropogenic factors that will radically alter northern forests in 50 years 3Pocket-sized anthrax detector aids global agriculture 2Pocket-sized anthrax detector aids global agriculture 3
... Two years ago, a group of friends were enjoying ... Germany when their conversation turned to the health benefits which ... making wine, by-products are left over which are often just ... by-products contain the goodness of wine in an even more ...
... presented in the name of Engineering Conferences International ... be awarded to Professor Sakayu Shimizu of Kyoto ... both fundamental and applied sciences. He has contributed ... functions, reactions and enzymes as biotechnological tools for ...
... by Hebrew University of Jerusalem researchers of an additional role ... step in world-wide efforts to develop genetic regulation aimed at ... The molecule, known as Lysyl-tRNA synthetase (or LysRS ... the cell, where it has long been recognized for its ...
Cached Biology News:The wonders of wine 2The wonders of wine 3Sakayu Shimizu of Kyoto University recipient of 2009 Enzyme Engineering Award 2
(Date:1/14/2014)... Carahsoft and CDS Federal Services have scheduled a ... EST (11am PST), “Natural Language Processing: Converting Raw Data ... can turn raw, heterogeneous data into actionable knowledge to ... webinar will last approximately one hour. , Synopsis: Big ...
(Date:1/14/2014)... Texas , Jan. 14, 2014 The ... and research medicinal plants and therapeutic derivatives thereof ... health professionals, and researchers about the challenges of ... http://photos.prnewswire.com/prnh/20100430/DC95601LOGO ) The Society ...
(Date:1/14/2014)... January 14, 2014 Global Record ... innovative information technology solutions for patients, physicians, the ... stakeholders, announced today the signing of a three-year ... and Drug Administration (FDA). This initiative is ...
(Date:1/14/2014)... Altadena, CA (PRWEB) January 14, 2014 ... Angeles County), California, and Montreal, Canada, has big ideas ... and non-fiction. The press's goals are to produce high-quality, ... e-books to support local businesses. , The first ...
Breaking Biology Technology:Webcast - Natural Language Processing: Converting Raw Data into Actionable Knowledge – Hosted by Carahsoft and CDS Federal Services 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 3World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 4World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 5World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 6World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 7World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 8Global Record Systems Announces Research Collaboration Agreement with FDA to Create a Novel “Big Data” Paradigm for Collection of Patient Safety and Outcomes Information 2Bitingduck Press Looks Forward to 2014 With Multimedia E-books 2
... PDL BioPharma, Inc. (PDL) (Nasdaq: PDLI ) today announced ... officer, will present at the Oppenheimer 20th Annual Healthcare Conference ... 9:40 a.m. Eastern Time , To access the live and ... company,s website at http://www.pdl.com and click "Investors." Please ...
... 27 Organogenesis, Inc. Chief Medical Officer Dr. Damien ... prestigious TEDMED medical innovation conference beginning today in San ... Come From?" will outline developments in the emerging and ... of patient lives. , The field of regenerative medicine ...
... PRINCETON, N.J., Oct. 27 Soligenix, Inc., (Soligenix or ... DOR BioPharma, Inc., a late-stage biotechnology company, announced today ... a company overview highlighting recent developments at the BIO ... Thursday, October 29, 2009 at 1:00 PM PDT at ...
Cached Biology Technology:Dr. Damien Bates of Organogenesis Speaks on Regenerative Medicine for Opening Night of TEDMED Conference 2Soligenix to Present at 8th Annual BIO Investor Forum 2Soligenix to Present at 8th Annual BIO Investor Forum 3Soligenix to Present at 8th Annual BIO Investor Forum 4
Human SDNSF/MCFD2 Biotinylated Affinity Purified PAb...
dithiothreitol, sulfhydryl reducing agent, C4H10O2S2; MW: 154.24, 97% pure (minimum), CAS No: 27565-41-9, store at -20 C ...
Human SCP3 Affinity Purified Polyclonal Ab Keywords: Spermatogenesis, meiosis Protein Family: Cell Cycle...
Request Info...
Biology Products: