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Rice University researchers optimize photoluminescent probes to study DNA and more
Date:9/6/2012

he hairpin-shaped probes' middles are designed to attach to a specific DNA sequence, while the ends are of opposite natures. One carries the fluorophore (iridium or ruthenium), the other a chemical quencher that keeps the fluorescence in check until the probe latches onto the DNA. When that happens, the fluorophore and the quencher are pulled apart and the probe lights up.

The individual signal is a flash too tiny and quick for the naked eye to see. "But our instruments can," Mart said. "We're trying to show that you can use time-resolved spectroscopy for many applications, but to use it in the right way, you have to do some analysis first," he said. "If you do it in the correct way, then it's a very powerful technique."


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Contact: David Ruth
david@rice.edu
713-348-6327
Rice University
Source:Eurekalert

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