Navigation Links
Rice University researchers optimize photoluminescent probes to study DNA and more
Date:9/6/2012

ctroscopy, it's like taking a film instead of a snapshot. "We create a kind of movie that allows us to see a specific moment in the process where photoluminescence is occurring. Then we can filter out the shadows that obscure the measurement or spectra we're looking for," he said.

With samples loaded into the spectrometer, researchers yell "Action!" by firing a laser that excites the target. In an edit of the resulting "movie" (which can be done in real time by the spectrometer), they chop off the front and back to narrow the data set to a range that might last only 80-billionths of a second, when the probe signal is strongest and the background signals are absent.

But it's critical to know just the right window of time to look at, Mart said. That's where the Rice methodology removes any uncertainty. They let researchers analyze all the factors, such as the emission intensity and decay of the specific probe with and without the target and the anticipated level of background noise. The experiment can then maximize the signal-to-background noise ratio. The technique works even with probes that are less than optimal, he said.

In combination with a technique called fluorescence lifetime microscopy, the Rice calculations may improve results from other diagnostic tools that gather data over time, such as magnetic resonance imaging machines used by hospitals.

Mart said the equations were the common-sense results of years of working with fluorescent spectroscopy. But, he said, when he looked for materials to help teach his students how to use time-resolved techniques to improve probes' resolution, he found none.

"I thought there must be some publication out there that would describe the tools we use, but there weren't any," he said. "So we've had to write them."

To prove their method, Mart and Huang tested ruthenium- and iridium-based light-switching probes under standard fluorescent and time-resolved spectroscopy. T
'/>"/>

Contact: David Ruth
david@rice.edu
713-348-6327
Rice University
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. University of Tennessee Space Institute researchers make clinical trials a virtual reality
2. University of Leicester takes delivery of unique eye imaging equipment
3. University of Tennessee Team receives NSF support to study toxic water in China
4. George Washington University Computational Biology Director solves 200-year-old oceanic mystery
5. University of Minnesota engineering researchers discover new non-invasive method for diagnosing epilepsy
6. Prestigious Royal Society of Tropical Medicine and Hygiene journals join Oxford University Press
7. MDA supports Duchenne muscular dystrophy research by University of Nevada School of Medicine
8. University of Minnesota receives $13.1 million in DOE funding for 2 new nationwide centers
9. University of Toronto wins third place at the Gates Foundations Reinvent the Toilet Challenge
10. NIH backs Rice University study of delay in gene transcription networks
11. New forensic institute at the University of Leicester will help police forces solve unusual crimes
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/28/2014)... Invasives is now available from the Lady Bird Johnson ... for identifying harmful non-native plant, insect and other invasive ... States cause about $137 billion in economic and other ... kill native plants and more. , "By having streamlined ... invasive species occur, we,re more likely to catch these ...
(Date:8/28/2014)... The Entomological Society of America (ESA) is pleased to ... Honorary Member of the Society. Honorary Membership acknowledges those ... through significant involvement in the affairs of the Society ... honor are selected by the ESA Governing Board and ... will be honored at the Awards Ceremony at Entomology ...
(Date:8/28/2014)... handful of bacterial culprits that may drive inflammatory bowel ... using patients, own intestinal immune responses as a guide. ... journal Cell . , Trillions of bacteria exist ... role in the development and progression of IBD. Yet ... species affect a person,s susceptibility to IBD and its ...
Breaking Biology News(10 mins):Mobile app makes ID of harmful plants, insects in Texas a snap 2Fred Baxendale selected as Honorary Member of the Entomological Society of America 2Yale study identifies possible bacterial drivers of IBD 2
... better understanding how antimicrobials bind and thereby get inactivated ... University of Illinois may have found a way to ... not a cure, this work has potential as a ... Gerard Wong a professor of materials science and engineering, ...
... nodes can be crucial for spreading low doses of ... as scrapie and Creutzfeldt-Jakob disease - into the nervous ... open access journal BMC Veterinary Research. While we can ... role in the neuroinvasion of prions for high doses ...
... caused the extinction of mammoths and the decline of ... debated. Overhunting by Paleoindians, climate change and disease lead ... considered a little out there is now hitting closer ... including two Northern Arizona University geologists, reports evidence that ...
Cached Biology News:Cystic fibrosis patients may breathe easier, thanks to bioengineered antimicrobials 2Lymph nodes can be key in spreading prion infectivity 2Research team says extraterrestrial impact to blame for Ice Age extinctions 2Research team says extraterrestrial impact to blame for Ice Age extinctions 3
(Date:8/27/2014)... YORK , Aug. 27, 2014 /PRNewswire/ ... research report is available in its catalogue: ... 2014-2018 http://www.reportlinker.com/p02316661/Global-Biodegradable-Packaging-Materials-Market-2014-2018.html ... materials are materials that are readily decomposed ... differs from conventional non-degradable packaging in terms ...
(Date:8/27/2014)... The Global and China Trifluoroacetic Acid Industry is ... of the Trifluoroacetic Acid industry in Global and China. ... industry, including definitions, applications and industry chain structure. Global ... with a focus on history, developments, trends and competitive ... and Chinese situation is also offered. , The report ...
(Date:8/27/2014)... Aug. 27, 2014  Lisa Kulik saw fireworks for ... 4 holiday, thanks to a groundbreaking retinal implant co-invented ... renowned clinician-researcher at the University of Southern California (USC) ... Peoria, Ariz. resident who has retinitis ... of sight. On June 2, she became the first ...
(Date:8/27/2014)... NEW YORK , Aug. 27, 2014 ... of Guggenheim Partners, today announced the hiring of veteran ... and senior equity analyst. Mr. Butler will focus on ... brings our team a wealth of experience and a ... Dante Ferrarie , Senior Managing Director and Head of ...
Breaking Biology Technology:Global Biodegradable Packaging Materials Market 2014-2018 2Global Biodegradable Packaging Materials Market 2014-2018 3Global Biodegradable Packaging Materials Market 2014-2018 4Global Biodegradable Packaging Materials Market 2014-2018 5Global Biodegradable Packaging Materials Market 2014-2018 6Global Biodegradable Packaging Materials Market 2014-2018 7Trifluoroacetic Acid Industry (Asia, Europe, North America, ROW) Analysis Report Now Available at ReportsnReports.com 2USC Eye Institute ophthalmologists implant first FDA-approved Argus II retinal prosthesis in western United States 2USC Eye Institute ophthalmologists implant first FDA-approved Argus II retinal prosthesis in western United States 3USC Eye Institute ophthalmologists implant first FDA-approved Argus II retinal prosthesis in western United States 4USC Eye Institute ophthalmologists implant first FDA-approved Argus II retinal prosthesis in western United States 5Guggenheim Securities Hires Analyst Charles "Tony" Butler to Expand Healthcare Coverage 2
... Emmanuel Labourier, Marianna Goldrick, Ambion, ... that mediate post-transcriptional gene regulation by pairing , ... untranslated region of messenger RNAs and acting , ... and exciting area of research with applications ...
... High Density Oligonucleotide , Microarrays , ... engineered to maximize yields of full-length cDNA , ... 100 ng of input RNA in a single round for GeneChip , ... cRNA synthesis powered by , ...
... , Dmitriy Ovcharenko, Rich Jarvis, ... Ambion, Inc. , , ... is a powerful , experimental ... genes. It is used routinely for gene function analysis, target , ...
Cached Biology Technology:miRNA Expression in White Blood Cells 2miRNA Expression in White Blood Cells 3miRNA Expression in White Blood Cells 4miRNA Expression in White Blood Cells 5Improving RNA Amplification 2Improving RNA Amplification 3Improving RNA Amplification 4Improving RNA Amplification 5Improving RNA Amplification 6Improving RNA Amplification 7Improving RNA Amplification 8Improving RNA Amplification 9Improving RNA Amplification 10Improving RNA Amplification 11Improving RNA Amplification 12Improving RNA Amplification 13Improving RNA Amplification 14Delivering siRNAs to Difficult Cell Types 2Delivering siRNAs to Difficult Cell Types 3Delivering siRNAs to Difficult Cell Types 4Delivering siRNAs to Difficult Cell Types 5Delivering siRNAs to Difficult Cell Types 6Delivering siRNAs to Difficult Cell Types 7
...
... positively and negatively supercoiled DNA in presence of ... the effects of supercoiling on transcription in vitro ... , Determining the degree of supercoiling of naturally ... differ in length by only one base-pair ...
Required to perform reference file generation, flatfield calibration and performance verification processes on the GSD-501...
Applications: Immunohistochemistry, ISH, FISH, CISH....
Biology Products: