Navigation Links
Revealing the secrets of motility in archaea
Date:2/14/2013

a platform to which FlaI attaches and also forms a kind of bearing that penetrates the cell membrane, and FlaB, the subunits of the archaellum filament itself, plus other helper proteins.

"Just how ATPases catch the energy of ATP is a long-standing question," says Tainer. The individual monomers in the FlaI crown process the ATP and reduce it to ADP by releasing a phosphate; here the "glue" that binds a FlaI monomer to its neighbor in the crown lets go. "In this phosphate release mechanism our team including Abrahjyoti Ghosh and Gareth Williams in Life Sciences were able to see a state never seen before, an intermediate conformation created by the released phosophate."

Instantly the whole FlaI monomer in the crown moves upward, pushing up the FlaB filament and opening a gap at its base where waiting FlaB subunits are added to the filament, causing it to grow. The process is similar to the mechanism in a bacterial Type IV pilus, although the resulting structures operate very differently.

That FlaI is the protein uniquely responsible for both assembly and motility of the archaellum was established by further genetic studies. A mutant strain that lacked only the first 29 amino acids in the NTD point was quite capable of assembling archaella, but incapable of making them rotate.

"This mutant was interesting to us because it raised the question of how altered FlaI proteins keep the ability to assemble archaella, yet they lose their motility," says Albers. "That the N-terminal-deletion mutant can assemble archaella, but the cells are not motile, implies that after assembly a switch is thrown, and the filament starts to rotate."

The biggest challenge for the team's continuing research is to learn how, as ATP releases energy, the upward movement of FlaI monomers is transferred to the rotation of the archaellum filament. Does the hexamer the whole FlaI crown rotate, or only the archaellum itself? And if so, how?

<
'/>"/>
Contact: Paul Preuss
paul_preuss@lbl.gov
510-486-6249
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3 4 5

Related biology news :

1. Prehistoric ghosts revealing new details
2. Revealing climate change website wins global award
3. The secrets of a tadpoles tail and the implications for human healing
4. Secrets of gentle touch revealed
5. Biologists unlocking the secrets of plant defenses, 1 piece at a time
6. Pond skating insects reveal water-walking secrets
7. Biophysicists unravel secrets of genetic switch
8. Ancient genome reveals its secrets
9. New model gives hands-on help for learning the secrets of molecules
10. Decoding the secrets of balance
11. Hidden secrets in the worlds most northerly rainforests
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Revealing the secrets of motility in archaea
(Date:8/4/2015)... ) today reported financial and operating results for the second quarter ... quarter contract revenue of $ 85.2 million, a ... of 26 % , Second quarter adjusted ... royalties in the current quarter , Operating cash ... to present another strong financial quarter, with all our divisions achieving ...
(Date:7/31/2015)... SHENZHEN, China , 31 de julio de 2015 ... sobre Genómica (ICG-10,  www.icg-10.org ) del 22 al 25 ... . La conferencia celebra su décimo ... se ha convertido en una de las reuniones anuales ... ,ómicas, y es una de las conferencias científicas más ...
(Date:7/31/2015)... de julio de 2015 La 10 th ... por medio de BGI del 22 al 25 de octubre de ... Este año, la conferencia celebra su décimo aniversario. Desde ... en una de las reuniones anuales más influyentes del mundo ... reuniones más dinámicas, entusiastas y mejores a nivel científico. ...
Breaking Biology News(10 mins):AMRI Announces Second Quarter 2015 Results 2AMRI Announces Second Quarter 2015 Results 3AMRI Announces Second Quarter 2015 Results 4AMRI Announces Second Quarter 2015 Results 5AMRI Announces Second Quarter 2015 Results 6AMRI Announces Second Quarter 2015 Results 7AMRI Announces Second Quarter 2015 Results 8AMRI Announces Second Quarter 2015 Results 9AMRI Announces Second Quarter 2015 Results 10AMRI Announces Second Quarter 2015 Results 11AMRI Announces Second Quarter 2015 Results 12AMRI Announces Second Quarter 2015 Results 13AMRI Announces Second Quarter 2015 Results 14AMRI Announces Second Quarter 2015 Results 15AMRI Announces Second Quarter 2015 Results 16AMRI Announces Second Quarter 2015 Results 17AMRI Announces Second Quarter 2015 Results 18AMRI Announces Second Quarter 2015 Results 19AMRI Announces Second Quarter 2015 Results 20AMRI Announces Second Quarter 2015 Results 21AMRI Announces Second Quarter 2015 Results 22AMRI Announces Second Quarter 2015 Results 23AMRI Announces Second Quarter 2015 Results 24La 10ª Conferencia Internacional sobre Genómica (ICG-10) comienza en octubre 2La 10ª Conferencia Internacional sobre Genómica (ICG-10) comienza en octubre 3La 10th International Conference on Genomics (ICG-10) se llevará a cabo en octubre 2La 10th International Conference on Genomics (ICG-10) se llevará a cabo en octubre 3
... A Duke University study suggests that evolution can behave ... depend on an energy-efficient style of four-footed running over ... have evolved a profoundly inefficient gait, tailor-made to creep ... "It is usually assumed that efficiency is what ...
... Using a harmless virus to insert a corrective gene ... Research Hospital have alleviated sickle cell disease pathology. In ... showed essentially no difference from normal mice. Although the ... presents significant technical obstacles, they believe that the new ...
... failing to take their medication for a range of ... a new university research project. The 21-month study ... The University of Leicester will draw on the experiences ... and epilepsy to learn about the barriers that prevent ...
Cached Biology News:Dogs chase efficiently, but cats skulk counterintuitively 2Gene therapy corrects sickle cell disease in laboratory study 2Gene therapy corrects sickle cell disease in laboratory study 3A spoonful of sugar? 2A spoonful of sugar? 3
(Date:8/28/2015)... ... August 28, 2015 , ... Riordan-McKenna Institute ... announced today that the use of sterile, dehydrated amniotic tissue AlphaPATCH™ developed by ... otherwise non-healing surgical knee wound. , The case involved a 78-year-old male, who ...
(Date:8/27/2015)... ... August 27, 2015 , ... ... venture capital funds, investment firms and animal health companies to hear from animal ... $160 million. Several have also received licensing agreements or distribution contracts. This ...
(Date:8/27/2015)... 27, 2015  Neogen Corporation (NASDAQ: NEOG ) ... United Kingdom -based Lab M Holdings, ... and diagnostic systems. Lab M was ... provider of microbial testing and diagnostic products for the ... company currently sells into more than 70 countries worldwide, ...
(Date:8/27/2015)... -- The National Necrotizing Fasciitis Foundation (NNFF) ... doctors at Vanderbilt University Hospital in Nashville, ... Dr. John Crew , director of the ... in Daly City, CA. ... by Dr. Crew, that had previously saved the lives ...
Breaking Biology Technology:Dallas-Fort Worth Stem Cell Researchers Use Amniotic Tissue To Successfully Treat Non-Healing Surgical Wound 2Dallas-Fort Worth Stem Cell Researchers Use Amniotic Tissue To Successfully Treat Non-Healing Surgical Wound 3Dallas-Fort Worth Stem Cell Researchers Use Amniotic Tissue To Successfully Treat Non-Healing Surgical Wound 4VetStem Biopharma, Inc. Among Seventeen Companies Selected to Present at the KC Animal Health Investment Forum on September 1st 2015 2VetStem Biopharma, Inc. Among Seventeen Companies Selected to Present at the KC Animal Health Investment Forum on September 1st 2015 3Neogen acquires U.K.-based Lab M Holdings 2Patient's Life and Eyes Saved from Gruesome Flesh-Eating Infection Consuming Her Face 2Patient's Life and Eyes Saved from Gruesome Flesh-Eating Infection Consuming Her Face 3
... SAN DIEGO, Oct. 16 CardioDynamics (Nasdaq:,CDIC), ... technology,has been named to Deloitte,s prestigious Technology Fast ... 50 fastest growing technology, media,telecommunications, and life sciences ... LLP, one of the nation,s leading professional services,organizations. ...
... Rigel,Pharmaceuticals, Inc. (Nasdaq: RIGL ) today ... presentations at the AACR-NCI-EORTC,International Conference on Molecular Targets ... They will highlight drug discovery,efforts focused on significant ... like kinase 1 (PLK1) and Janus tyrosine kinase,2 ...
... Stockholder Meeting October 23, IRVINE, California, October ... an orthobiologics company, today announced the,interim tabulation results ... 2007 and adjourned to October 23, 2007. ... 36 percent,of the shares entitled to vote, voted ...
Cached Biology Technology:CardioDynamics Ranked in Deloitte's Technology Fast 50 Program for San Diego 2CardioDynamics Ranked in Deloitte's Technology Fast 50 Program for San Diego 3CardioDynamics Ranked in Deloitte's Technology Fast 50 Program for San Diego 4Rigel to Present Research Programs at Scientific Conferences 2Rigel to Present Research Programs at Scientific Conferences 3IsoTis Reports Interim Results of Vote for Integra Merger 2IsoTis Reports Interim Results of Vote for Integra Merger 3IsoTis Reports Interim Results of Vote for Integra Merger 4
... The OneSTrEP Protein Interaction Kit provides ... step method to isolate intact protein ... tedious optimization or multiple purification steps, ... highly selective StrepTactin Superflow Column for ...
Request Info...
... The system consists ... (Cat. No. 17001-082) and ... 17002-080). It is complete ... promoting agents, and requires ...
... The product contains 5 ml Dynabeads Tosylactivated ... p-toluenesulphonyl (tosyl) reactive groups. For coupling of ... hydrophobically on initial coupling with covalent binding ... and sulphydryll groups (SH)) occurringovernight. Coupling reactions ...
Biology Products: