Navigation Links
Revealing the secrets of motility in archaea
Date:2/14/2013

a platform to which FlaI attaches and also forms a kind of bearing that penetrates the cell membrane, and FlaB, the subunits of the archaellum filament itself, plus other helper proteins.

"Just how ATPases catch the energy of ATP is a long-standing question," says Tainer. The individual monomers in the FlaI crown process the ATP and reduce it to ADP by releasing a phosphate; here the "glue" that binds a FlaI monomer to its neighbor in the crown lets go. "In this phosphate release mechanism our team including Abrahjyoti Ghosh and Gareth Williams in Life Sciences were able to see a state never seen before, an intermediate conformation created by the released phosophate."

Instantly the whole FlaI monomer in the crown moves upward, pushing up the FlaB filament and opening a gap at its base where waiting FlaB subunits are added to the filament, causing it to grow. The process is similar to the mechanism in a bacterial Type IV pilus, although the resulting structures operate very differently.

That FlaI is the protein uniquely responsible for both assembly and motility of the archaellum was established by further genetic studies. A mutant strain that lacked only the first 29 amino acids in the NTD point was quite capable of assembling archaella, but incapable of making them rotate.

"This mutant was interesting to us because it raised the question of how altered FlaI proteins keep the ability to assemble archaella, yet they lose their motility," says Albers. "That the N-terminal-deletion mutant can assemble archaella, but the cells are not motile, implies that after assembly a switch is thrown, and the filament starts to rotate."

The biggest challenge for the team's continuing research is to learn how, as ATP releases energy, the upward movement of FlaI monomers is transferred to the rotation of the archaellum filament. Does the hexamer the whole FlaI crown rotate, or only the archaellum itself? And if so, how?

<
'/>"/>
Contact: Paul Preuss
paul_preuss@lbl.gov
510-486-6249
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3 4 5

Related biology news :

1. Prehistoric ghosts revealing new details
2. Revealing climate change website wins global award
3. The secrets of a tadpoles tail and the implications for human healing
4. Secrets of gentle touch revealed
5. Biologists unlocking the secrets of plant defenses, 1 piece at a time
6. Pond skating insects reveal water-walking secrets
7. Biophysicists unravel secrets of genetic switch
8. Ancient genome reveals its secrets
9. New model gives hands-on help for learning the secrets of molecules
10. Decoding the secrets of balance
11. Hidden secrets in the worlds most northerly rainforests
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Revealing the secrets of motility in archaea
(Date:7/28/2014)... and other forms of dementia, but the research community ... of Washington bioengineers have a designed a peptide structure ... normal proteins into a state that,s linked to widespread ... diabetes and Lou Gehrig,s disease. The synthetic molecule blocks ... into an abnormally folded form by targeting a toxic ...
(Date:7/28/2014)... research has identified areas of the Earth that are ... change. , Europe is particularly vulnerable, as it has ... per cent, of any continent in ,refugia, areas ... environmental conditions remain relatively constant during times of great ... are mostly in Scandinavia and Scotland. , The ...
(Date:7/28/2014)... St. Jude Children,s Research Hospital study found that 73 ... doubled their risk of developing metabolic syndrome and related ... The results appear in the current issue of the ... the 1,598 adult survivors of childhood cancer in the ... risk factors like high blood pressure, abdominal obesity, elevated ...
Breaking Biology News(10 mins):New protein structure could help treat Alzheimer's, related diseases 2New protein structure could help treat Alzheimer's, related diseases 3Study finds Europe's habitat and wildlife is vulnerable to climate change 2Unhealthy habits more than double risk of metabolic syndrome in childhood cancer survivors 2Unhealthy habits more than double risk of metabolic syndrome in childhood cancer survivors 3
... comparing the immune responses of both, young and old ... of macrophages, one of the major cell populations involved ... aged mice. This decline in the number of fighters ... be responsible for the age-associated increase in susceptibility to ...
... 2010 Burnham Institute for Medical Research (Burnham) announced ... million to support and further expand and accelerate the ... been renamed the Sanford-Burnham Medical Research Institute. This is ... 2007, he directed $20 million through Sanford Health to ...
... FAIRFAX, Va.Nearly 5,300 physicians, scientists and allied health ... Interventional Radiology,s 35th Annual Scientific Meeting March 13󈝾 ... are scheduled for 9󈝶:30 a.m. on both Monday, ... Annual Scientific Meeting offers ...
Cached Biology News:Rejuvenating the old immune system 2T. Denny Sanford donates $50 million to Burnham Institute for Medical Research 2Press registration open for Society of Interventional Radiology’s 35th Annual Scientific Meeting 2
(Date:7/28/2014)... and Annapolis Junction, MD (PRWEB) July 28, 2014 ... research leader in personalized medicine, will be exhibiting data ... at this year’s International Spine Intervention Society’s ... place July 30th-August 3rd, at the Hyatt Regency, in ... to enhance the knowledge and clinical competence of physicians ...
(Date:7/28/2014)... TORONTO , July 28, 2014 /PRNewswire/ -   ... latest group of companies to join its growing portfolio ... hallmarks of Toronto,s JOLT accelerator, ... startups on the roster. Since its ... that have successfully raised  $7.5M of financing. Now at ...
(Date:7/28/2014)... 28, 2014  David Hardison, Ph.D., vice president ... , has been elected chairman of the board ... non-profit working to establish common protocols around the ... relates to electronic health records. ... collaborating with organizations across the research and health ...
(Date:7/28/2014)...   Propeller Health , the FDA-cleared digital health ... Brad Towle as Senior Vice President, Business ... the company,s sales growth, customer acquisition and program expansion. ... his past role in the development of accountable care ... pivotal time in the industry," said David Van ...
Breaking Biology Technology:Proove Biosciences Exhibits Industry Leading Data and Research at International Spine Intervention Society’s 22nd Annual Scientific Meeting 2JOLT Announces the Next 6 Startups to Join its Summer 2014 Cohort 2JOLT Announces the Next 6 Startups to Join its Summer 2014 Cohort 3ConvergeHEALTH by Deloitte's David Hardison Named Chairman of the Clinical Data Interchange Standards Consortium 2ConvergeHEALTH by Deloitte's David Hardison Named Chairman of the Clinical Data Interchange Standards Consortium 3Propeller Health Hires Brad Towle as Senior Vice President of Business and Client Development 2
... Hefty has seen blue-ribbon government panels come and go ... thick file of reports spanning 20 years of commissions, ... thoughtful research papers is only useful if it leads ... Hefty, who retired last year from Blue-Cross/Blue Shield United ...
... Role in Community...promotes entertainment everywhere on any device ... approval for the sale of the companys desktop products ... (SPD) The transaction is now complete and Sony Pictures ... it will keep the companys employee base in Madison ...
... alternative energy , ,Madison, WI - WE Energies ... two developers that will build three wind farms increasing ... by 400%. , ,Under the agreement, the two developers, ... LLC, based in Chicago, will provide their entire energy ...
Cached Biology Technology:Governors Growth Council hopes to go light on paperwork and heavy on ideas 2Governors Growth Council hopes to go light on paperwork and heavy on ideas 3Sony Completes Acquisition of Sonic Foundry 2Sony Completes Acquisition of Sonic Foundry 3WE Energies Is Not Letting Wind Power Blow Away 2
GeneRacer is an advanced RACE (rapid amplification of cDNA ends) technique that improves the efficiency of amplifying full-length 5' and 3' cDNA ends. With the GeneRacer Kit you can:...
One-step, microplate or cuvet, colorimetric, detection range 0.04% to 4%. Procedure: 30 min....
... 26] to Presenilin 2 Alzheimer's disease ... the disease carry mutations in the presenilin ... protein (APP). These disease-linked mutations result in ... amyloid-beta (main component of amyloid deposits found ...
One-step, microplate or cuvet, colorimetric, linear detection range 0.7 mg/dL to 35 mg/dL. Procedure: 5 min....
Biology Products: