Navigation Links
Revealing the secrets of motility in archaea
Date:2/14/2013

find proof. FlaI is an ATPase an enzyme that releases energy from adenosine triphosphate, or ATP and was known to be involved in the assembly and function of Type IV pili in bacteria and the secretion of proteins in many microorganisms. But FlaI's role in archaella was uncertain.

The Albers lab tested what happened to S. acidocaldarius when FlaI genes were deleted. Wild-type S. acidocaldarius cells have only one to three archaella on their surfaces, so "hyper-flagellated, hyper-motile" mutants with numerous archaella were created, to make deletion effects readily apparent. Deleting the FlaI gene eliminated the archaella and left the mutants unable to move. But when the researchers reintroduced the FlaI genes, the mutants were able to assemble archaella and use them to swim.

Now the challenge was to find out how FlaI performed its two functions, archaellum assembly and motility, by solving the FlaI protein structure. John Tainer of Berkeley Lab's Life Sciences Division (LSD) and the Scripps Research Institute's Department of Molecular Biology was co-leader of the research with Albers. The stable proteins of S. acidocaldarius, an extremophile able to withstand hot, acidic conditions, make it suitable for x-ray diffraction crystallography, and Tainer's postdoctoral fellow, Sophia Reindl, was able to crystallize the FlaI protein.

To do the crystallography, Reindl used beamline 8.3.1 at Berkeley Lab's Advanced Light Source (ALS), pinpointing the position of all the atoms in the FlaI protein and revealing that it consists of two parts. A globular C terminal domain, or CTD, is connected by a flexible linker to a more variable N terminal domain, or NTD, which constitutes a moveable tip.

As an energy-releasing enzyme the FlaI protein binds to an ATP nucleotide, from which it detaches a phosphate to generate energy leaving adenosine diphosphate, ADP, in its place. After releasing the ADP, the protein binds to a
'/>"/>

Contact: Paul Preuss
paul_preuss@lbl.gov
510-486-6249
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3 4 5

Related biology news :

1. Prehistoric ghosts revealing new details
2. Revealing climate change website wins global award
3. The secrets of a tadpoles tail and the implications for human healing
4. Secrets of gentle touch revealed
5. Biologists unlocking the secrets of plant defenses, 1 piece at a time
6. Pond skating insects reveal water-walking secrets
7. Biophysicists unravel secrets of genetic switch
8. Ancient genome reveals its secrets
9. New model gives hands-on help for learning the secrets of molecules
10. Decoding the secrets of balance
11. Hidden secrets in the worlds most northerly rainforests
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Revealing the secrets of motility in archaea
(Date:12/5/2014)... Calif. , Dec. 4, 2014 ... has boosted investments in new testing and inspection ... also given rise to a range of innovative ... the requirements of Generation Y, which is generally ... As a result, product development strategies of test ...
(Date:11/21/2014)... According to a new market research report ... Control, Intrusion Detection, Parking Management, Under Vehicle Inspection), End ... - Global Forecasts to 2020", published by MarketsandMarkets, The ... $25 Billion in 2014 and is expected to reach ... 8.69%. Browse 116 market data Tables and ...
(Date:11/18/2014)... 18, 2014  The Secure Identity & Biometrics ... jointly announce the formation of The Airport Entry ... Identity and Biometric Entry and Exit Solutions ... official support from BORDERPOL, the international non-profit organization ... provide expertise regarding border security, traveler and migration ...
Breaking Biology News(10 mins):Emerging Technologies Enable Smarter Monitoring of Assets, Push Industrial Testing, Inspection and Monitoring Market 2Emerging Technologies Enable Smarter Monitoring of Assets, Push Industrial Testing, Inspection and Monitoring Market 3Emerging Technologies Enable Smarter Monitoring of Assets, Push Industrial Testing, Inspection and Monitoring Market 4Industrial Security Systems Market Worth $38 Billion by 2020 2Industrial Security Systems Market Worth $38 Billion by 2020 3Identity Management, Document Authentication and Biometric Companies Form "The Airport Entry and Exit Working Group", Releasing the Framework to Help Guide the Department of Homeland Security and Congress Towards Solutions 2Identity Management, Document Authentication and Biometric Companies Form "The Airport Entry and Exit Working Group", Releasing the Framework to Help Guide the Department of Homeland Security and Congress Towards Solutions 3
... Institute near Cambridge, supported by the Alzheimer,s Research ... Council (BBSRC), have discovered that the brain,s circuitry ... ageing such as Alzheimer,s disease. The findings were ... Alzheimer,s disease causes nerve cells in the ...
... The Stowers Institute,s Linheng Li Lab has identified the precise ... were published today in the Advance Online Publication of ... of the Institute,s support facilities to develop a new technology ... to monitor the dynamic behavior of stem cells. This technology ...
... NY, December 4, 2008Researchers are taking a closer look ... to demonstrate that inhibiting this biological route suppresses tumor ... ( GEN ). The pathway is involved in angiogenesis, ... part of the PI3/AKT/mTOR pathway, which plays a key ...
Cached Biology News:Maintaining the brain's wiring in aging and disease 2Stowers Institute's Linheng Li Lab expands understanding of bone marrow stem cell niche 2
(Date:12/15/2014)... (PRWEB) December 15, 2014 METTLER ... inline PVM tool, ParticleView V19 with PVM ... situ probe-based particle vision and measurement tool continuously ... process conditions. ParticleView V19 then automatically prepares a ... tracking particle size and concentration changes. This compelling ...
(Date:12/15/2014)... , Dec. 15, 2014  Ascendis Pharma A/S, ... technology to address significant unmet medical needs, today ... Phase 2 pediatric study to evaluate once-weekly TransCon ... or GHD.  This interim analysis consists of 25 ... enrollment in the study, completing all six months ...
(Date:12/13/2014)... 12, 2014 Trend lines for photonics ... global economic growth, Eugene Arthurs, CEO of SPIE, ... in a featured talk at OPTIC (Optics and Photonics ... National Chung Hsing University in Taichung. , As examples, ... future energy supplies and soon to hit US$50 billion ...
(Date:12/13/2014)... , Dec. 11, 2014 Research and ... the addition of the "Glucose Sensors: the ... http://photos.prnewswire.com/prnh/20130307/600769 ... product designs is giving hope for a better ... glucose monitors that can measure glucose levels accurately ...
Breaking Biology Technology:New Real-Time In Situ Probe-Based Video Microscope from METTLER TOLEDO 2Ascendis Pharma A/S Announces Positive Six-Month Interim Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 2Ascendis Pharma A/S Announces Positive Six-Month Interim Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 3Ascendis Pharma A/S Announces Positive Six-Month Interim Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 4Photonics Continues Upward Trends Despite Challenges in the Global Economy, Notes SPIE CEO 2Photonics Continues Upward Trends Despite Challenges in the Global Economy, Notes SPIE CEO 3Photonics Continues Upward Trends Despite Challenges in the Global Economy, Notes SPIE CEO 4Photonics Continues Upward Trends Despite Challenges in the Global Economy, Notes SPIE CEO 5Glucose Sensors: the Next Generation 2Glucose Sensors: the Next Generation 3
... 2 More than a dozen internationally renowned speakers, ... team, will be coming to New Haven, CT to ... symposium taking place March 23-24, 2009.The roster includes scientists ... creation of two new human embryonic stem cell lines ...
... Cephalon, Inc .,(Nasdaq: CEPH ) today ... worldwide rights to Lupuzor(TM), an investigational medication for the,treatment ... of the ImmuPharma Phase IIb study. Cephalon will assume ... commercialization of,the product. , ImmuPharma ...
... BioMed Realty Trust, Inc. (NYSE: BMR ) ... and year ended December 31, 2008 after the market closes ... Chief Executive Officer, and Kent Griffin, President and Chief Financial ... Pacific Time on Thursday, February 12, 2009 to discuss the ...
Cached Biology Technology:Obama Advisor Joins Connecticut and International Scientists at New Haven Stem Cell Symposium 2Cephalon Exercises Option to License Worldwide Rights to ImmuPharma Product, Lupuzor 2Cephalon Exercises Option to License Worldwide Rights to ImmuPharma Product, Lupuzor 3Cephalon Exercises Option to License Worldwide Rights to ImmuPharma Product, Lupuzor 4Cephalon Exercises Option to License Worldwide Rights to ImmuPharma Product, Lupuzor 5BioMed Realty Trust to Report 2008 Fourth Quarter and Year-End Results 2
Human Legumain Affinity Purified Polyclonal Ab...
Human Cystatin F MAb (Clone 292103)...
... Ac-DEVD-CHO is an inhibitor ... The concentration of inhibitor ... activity must be determined ... Ten micromolar inhibitor is ...
Human RELT/TNFRSF19L Affinity Purified Polyclonal Ab...
Biology Products: