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Researchers tap yeasts as source of 'green' surfactants
Date:7/28/2011

This release is available in Spanish.

Surfactants, which are wetting agents that lower a liquid's surface tension, have a long list of uses, from detergents and cosmetics to paints and pesticides. Most surfactants are petroleum-based. But in Peoria, Ill., a team of U.S. Department of Agriculture (USDA) scientists has focused their attention on sophorolipids, surfactant-like molecules produced by naturally occurring yeasts.

Microbiologist Cletus Kurtzman is spearheading the studies at the National Center for Agricultural Utilization Research (NCAUR), operated in Peoria by the Agricultural Research Service (ARS), USDA's chief intramural scientific research agency.

Using phylogenetic analysis and mass-spectrometry, Kurtzman's team screened 19 of the 40 known members of the Starmerella yeast family for their ability to produce the sophorolipids.

Phylogenetics, which traces the evolutionary relationships between species or groups, was particularly useful because it enabled the team to determine which Starmerella members produce the molecules based on shared gene sequences for the trait. For example, only a few Candida yeast species had previously been shown to make the sophorolipids-most notably, C. bombicola and C. apicola.

Kurtzman's team was able to broaden its search by tapping the ARS (microbial) Culture Collection, maintained at the Peoria center's Bacterial Foodborne Pathogens and Mycology Research Unit.

In studies there, the researchers cultured the yeasts on a diet of glucose oleic acid. They then measured the yeasts' sophorolipid production levels over a 24- to 168-hour period using mass-spectrometry analysis, which can identify compounds based on their unique molecular weights.

As expected, C. bombicola and C. apicola boasted the highest sophorol
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Contact: Jan Suszkiw
301-504-1630
United States Department of Agriculture - Research, Education and Economics
Source:Eurekalert

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