Navigation Links
Researchers study virus with unusual properties
Date:12/8/2008

ology who is now a postdoctoral researcher at the University of Rochester, used X-ray crystallography to obtain a high-resolution three-dimensional image of the N4 phage's RNA-polymerase and DNA molecule. "By modifying the crystallography conditions, Michael obtained an extremely high-resolution picture of the N4 RNA polymerase and DNA molecule. So we are able to analyze protein-DNA interactions much more clearly," said Murakami.

The picture of this RNA polymerase and DNA molecule has enabled the team to investigate how the RNA polymerase initiates transcription of phage DNA from inside a bacterial cell. "When a phage injects its DNA into a bacterial cell, the amount of its DNA is miniscule compared to the amount of host DNA," said Murakami. "We wanted to find out what prevents the N4 RNA polymerase from binding to the bacterial host's DNA rather than to the phage's DNA."

It turns out that the N4 RNA polymerase is able to respond only to DNA that is shaped like a hairpin. Part of the N4 phage's DNA is shaped like a hairpin, whereas the E. coli bacterium's DNA is not shaped like a hairpin. Once the N4 RNA polymerase interacts with the phage's hairpin DNA, it begins to change its shape from a fisted form to a cupped form. By opening up, the RNA polymerase exposes its active site, which allows it to begin the transcription process.

While the researchers determined that the N4 RNA polymerase must change its form in order to bind to the phage DNA, they also found that this transformation isn't the polymerase's first as it progresses through the steps of phage infection. The team found that the polymerase must change form in order to squeeze through the phage's tiny injection tube as it is injected into the E. coli cell. "The diameter of the tube is narrower than the diameter of RNA polymerase," said Murakami. "This means that the enzyme must be unfolded into a longer and thinner structure in order to fit through the tube
'/>"/>

Contact: Barbara K. Kennedy
science@psu.edu
814-863-4682
Penn State
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Key to curing obesity may lie in worms that destroy their own fat: McGill researchers
2. UC Davis researchers exploring gene therapy to fight AIDS
3. Researchers solve piece of large-scale gene silencing mystery
4. A little wine boosts omega-3 in the body: Researchers find a novel mechanism for a healthier heart
5. Researchers examine role of soil patterns in dam restoration
6. USC researchers head global effort to study genetic risks that contribute to psychiatric diseases
7. PNNL researchers earn top honors at Supercomputing conference
8. Einstein researchers develop technique to count messages made by single genes
9. House Ear Institute, TGen and Belgian researchers identify gene in age-related hearing loss
10. Researchers learn that some good cholesterol isnt good enough
11. URI researchers help score knockout punch on birch tree pest
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Researchers study virus with unusual properties
(Date:7/21/2015)... OXFORD, Connecticut , July 21, 2015 /PRNewswire/ ... the "Company"), a biometric authentication company focused on ... the Wocket® smart wallet, announces that it has ... Tokenization System and Method. This ... that not only authorizes an account, but also ...
(Date:7/13/2015)... , July 13, 2015 Synaptics Inc. ... human interface solutions, today announced sampling of ClearPad ... display driver integration (TDDI) product targeting smartphones and ... to combine Synaptics , best-in-class touch controller ... driver technology developed in the company,s Japan Design ...
(Date:7/9/2015)... 2015 Research and ... the "Biometrics for Banking; Market & Technology ... to their offering. The adoption for ... growth and the forecast is that by 2020 ... companies involved in delivering biometric systems to the ...
Breaking Biology News(10 mins):NXT-ID Patents Personalized Tokenized Payments for Next Generation Secure Payment Technology 2NXT-ID Patents Personalized Tokenized Payments for Next Generation Secure Payment Technology 3NXT-ID Patents Personalized Tokenized Payments for Next Generation Secure Payment Technology 4Synaptics Announces Sampling of Second Generation TDDI Solutions 2Synaptics Announces Sampling of Second Generation TDDI Solutions 3Global Banking Biometrics Market 2015-2020 - Market & Technology Analysis, Adoption Strategies and Forecasts 2Global Banking Biometrics Market 2015-2020 - Market & Technology Analysis, Adoption Strategies and Forecasts 3
... elderly people from benefiting equally from exercise, no matter ... published in today's (Aug. 10) Journal of the American ... who exercised stayed healthier than their couch potato peers, ... most from physical activity, said Marco Pahor, M.D., director ...
... a single gene, Nrf2, plays a critical role in ... by Shyam Biswal, PhD, at the Johns Hopkins Bloomberg ... exacerbated allergen-mediated asthma in mice models. The study’s findings, ... Journal of Experimental Medicine, may hold therapeutic potential for ...
... future hopes for human applications , Israeli scientists report ... successfully transplanted whole frozen and thawed ovaries in sheep, ... the laboratory into early embryonic development. , Follow-up tests ... which oocytes were recovered were still functioning normally three ...
Cached Biology News:Exercise aside, genes may ultimately dictate seniors' mobility 2Exercise aside, genes may ultimately dictate seniors' mobility 3Researchers Discover Gene That Determines Asthma Susceptibility By Regulating Inflammation 2Israeli scientists successfully transplant frozen-thawed ovaries in sheep 2Israeli scientists successfully transplant frozen-thawed ovaries in sheep 3
(Date:8/4/2015)... -- New open access, ... serving the international surgical community ... technical, and medical information products and services, today announced ... Surgery Open ( IJS Open ), a ... This peer-reviewed, online-only journal will make significant contributions to ...
(Date:8/4/2015)... (PRWEB) , ... August 04, 2015 , ... On August ... inviting a diverse selection of entrepreneurs from across the United States to talk about ... Richman is honored to be chosen to be among the attendees. , One of ...
(Date:8/3/2015)... 3, 2015  Halozyme Therapeutics, Inc. (NASDAQ: HALO ... the second quarter 2015 on Monday, August 10, 2015 ... Torley , president and chief executive officer, will lead ... release financial results for the second quarter 2015. ... "Investors" section of Halozyme,s corporate website and a recording ...
(Date:8/3/2015)... -- MiMedx Group, Inc. (NASDAQ: MDXG ), the leading ... processes to develop and market advanced products and therapies ... Ophthalmic and Dental sectors of healthcare, announced today that ... Canaccord|Genuity Growth Conference in Boston, Massachusetts ... William C. Taylor , President and COO, and ...
Breaking Biology Technology:Elsevier Announces the Launch of International Journal of Surgery Open 2Elsevier Announces the Launch of International Journal of Surgery Open 3Elsevier Announces the Launch of International Journal of Surgery Open 4White House Invites uBiome CEO Jessica Richman to First-Ever Startup Demo Day 2Halozyme to Host Second Quarter 2015 Financial Results Conference Call 2MiMedx To Present At 35th Annual Canaccord|Genuity Growth Conference 2
... (Nasdaq:,SIAL) announced today the global release of ... researchers to reproducibly,interrogate gene function, via siRNA ... not been considered amenable to conventional lipid-based,siRNA ... reagents exhibit a number,of drawbacks, including high ...
... DIEGO, Oct. 18 Volcano Corporation,(Nasdaq: VOLC ) ... shares of its common stock at a price of ... by Volcano. Volcano has granted the,underwriters a 30-day option ... common stock to cover over-allotments, if any. The offering,is ...
... Oct. 17 AMICAS, Inc. (Nasdaq: AMCS ),a ... today announced it will present to and meet with ... being held in New,York. (Logo: http://www.newscom.com/cgi-bin/prnh/20060202/AMICASLOGO ... -- Presentation begins promptly at 10:20 a.m. (Eastern ...
Cached Biology Technology:Sigma-Aldrich Introduces N-TER Nanoparticle siRNA Transfection System for Delivery of siRNA Into Difficult-to-Transfect Cell Types 2Volcano Corporation Announces Pricing of Common Stock Offering 2
... I (RNase-free) (E.C. 3.1.21.1) is a nonspecific ... chromatin. It functions by hydrolyzing phosphodiester linkages, ... and a 3'-hydroxyl group. Ambion's RNase-free DNase ... and is recommended to degrade DNA in ...
Rabbit polyclonal antibody to Neuromedin U Receptor 1 (GPR66)...
... Perhaps sheer in-house demand is causing ... want your precious antibody. This can cause ... • Receive royalties for your hard ... and let us build it up and ...
... (rat) - Diluted Antiserum for RIA, Host: ... U-23 (rat) Immunology Products, Host: Rabbit ... our corresponding peptide and 1 ... Neuromedin U-23 (rat) (H-9295) and ([ 1 ...
Biology Products: