Navigation Links
Researchers report advances vs. preeclampsia, including potential prediction
Date:10/14/2010

PROVIDENCE, R.I. [Brown University] In as many as 8 percent of pregnancies worldwide, women who seem fine for months develop preeclampsia, a serious complication causing symptoms including high blood pressure, severe swelling, and problems with placental development. The untreatable and unpredictable condition, with no known cause, often requires premature delivery, and can sometimes kill the mother and the fetus.

In a new study, researchers led by a team at Brown University and Women & Infants Hospital describe two major advances: a well-defined animal model of preeclampsia and a potential lab test for diagnosing the disease in people.

"Our model is the first pregnancy-specific animal model," said Surendra Sharma, professor of pediatrics at the Warren Alpert Medical School of Brown University and a research scientist at Women & Infants, "and our predictive assay is the first one where we can go back to the first trimester and predict problems."

Sharma is a senior author on the study, published online this month in The American Journal of Pathology. In addition to pediatrics researchers, the study also involved scientists at the Lifespan Center for International Health Research, Harvard Medical School in Boston, and Linkoping University and Helsingborg Hospital in Sweden.

Model mice

Building on research linking the presence of the immune system secretion Interleukin-10 (IL-10) to a successful course of pregnancy, the researchers started experimenting with mice genetically engineered to lack IL-10. They hypothesized that if they isolated blood serum from human patients with preeclampsia and gave a dose of it to the mice, the rodents would develop preeclampsia symptoms. That is exactly what happened.

Just to be sure, the researchers gave the preeclampsia serum to mice that were not pregnant. Nothing happened, confirming that the onset of preeclampsia symptoms in the engineered mice was a consequence of their pregnancy. Meanwhile, the researchers gave the preeclampsia serum to wild mice who were pregnant and found that they did not experience all the preeclampsia symptoms of mice without IL-10. That confirmed that pregnant mice lacking IL-10 provide a unique and dependable model of the disease.

The significance of having an animal model for preeclampsia is that it will allow for experiments that can shed light on the cause of the disease and its progression, Sharma said. That has already occurred in his research. For example, the team was able to observe something in the mice that is also observed in human preeclampsia patients: a disruption in the development of "spiral arteries," which bring nutrients to the fetus from the placenta.

Toward a diagnostic lab test

After observing how preeclampsia serum caused disruption to the spiral arteries, the team reasoned that preeclampsia serum might also disrupt the formation of vasculature in the lab. They created an in vitro culture of two key cell types involved in spiral artery development endothelial cells and trophoblasts and then exposed some to serum from women with normal pregnancies and some to preeclampsia serum from women taken at various stages of their pregnancies.

The researchers found that vasculature developed normally in the presence of serum from women with normal pregnancies. But they also found that preeclampsia serum taken from women as early as 12 to 14 weeks into their pregnancies, about 10 to 12 weeks before they were diagnosed with preeclampsia, was able to disrupt vascular formation.

Sharma has filed a patent application for the test and has continued to refine it for eventual clinical use, a process that will require FDA approval.

"The idea is that we can predict preeclampsia ahead of time and women can be treated," Sharma said. Researchers, for example, are looking at dysregulated proteins in preeclampsia serum for their causative effects and as one avenue of managing preeclampsia. The idea is that normal counterparts of these proteins will rescue normal pregnancy and protect against the onset of preeclampsia disease. "Hopefully, hopefully preeclampsia can be controlled," Sharma said.


'/>"/>

Contact: David Orenstein
david_orenstein@brown.edu
401-863-1862
Brown University
Source:Eurekalert

Related biology news :

1. Researchers develop oral delivery system to treat inflammatory bowel diseases
2. In Parkinsons disease, brain cells abandon mitochondria, researchers report
3. MIT researchers develop a better way to see molecules at work in living brain cells
4. Scripps researchers, UCSD chemists to create center devoted to chemistrys influence on climate
5. New fisheries system will save about $20 million, Iowa State University researchers find
6. The world is full of darkness, reflected in the physiology of the human retina, Penn researchers say
7. FSU researchers examine how bacteria become resistant to antibiotics
8. Knome Awards Human Exome Sequencing and Analysis to Biomedical Researchers
9. Researchers study sleep apnea and lack of oxygen
10. Bonn researchers use light to make the heart stumble
11. Researchers engineer adult stem cells that do not age
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/16/2016)... Research and Markets has announced the addition of the ... report to their offering. ... The biometric vehicle access system market, in terms of ... 2016 to 2021. The market is estimated to be USD 442.7 ... 2021. The growth of the biometric vehicle access system market is ...
(Date:12/15/2016)... , Dec. 15, 2016   WaferGen Bio-systems, Inc. ... genomics technology company, announced today that on December 13, ... Department of The Nasdaq Stock Market LLC which acknowledged ... price of WaferGen,s common stock had been at $1.00 ... has regained compliance with Listing Rule 5550(a)(2) of the ...
(Date:12/15/2016)... AUBURN HILLS, Mich. , Dec. 15, 2016 /PRNewswire/ ... simply unlocking car doors or starting the engine. Continental ... 2017 in Las Vegas . Through ... PASE (Passive Start and Entry) and biometric elements, the ... the field of vehicle personalization and authentication. ...
Breaking Biology News(10 mins):
(Date:1/19/2017)... , Jan. 18, 2017 The global ... USD 92.9 billion by 2025, according to a ... industry has been adaptive of the function of ... as 2002. Among the services outsourced, clinical trial ... instance, Johnson & Johnson was the first pharmaceutical ...
(Date:1/18/2017)... 2017 According to a new market research report "In ... Disease), & End User (Molecular Diagnostic Laboratories, Academic and Research Institutions) - Global ... 739.9 Million by 2021 from USD 557.1 Million in 2016, growing at a ... ... MarketsandMarkets Logo ...
(Date:1/18/2017)... ... January 18, 2017 , ... Total Orthopedics and Sports ... A-CIFT™ Solofuse-P™. The operation took place on Wednesday, January 11, 2017 at Long ... an anterior cervical discectomy and fusion on a 42 year old female who ...
(Date:1/18/2017)... ... January 18, 2017 , ... DrugDev customers ... Clinical Ops Executives (Hyatt Regency Miami, January 24-26). DrugDev will join customers including ... issues such as trial performance metrics, patient enrollment diversity, protocol optimization, and global ...
Breaking Biology Technology: