Navigation Links
Researchers insert identification codes into mouse embryos
Date:11/18/2010

Researchers from the Department of Cell Biology, Physiology and Immunology at Universitat Autnoma de Barcelona (UAB), in collaboration with researchers from the Institute of Microelectronics of Barcelona (IMB-CNM) of the Spanish National Research Council (CSIC), have developed an identification system for oocytes and embryos in which each can be individually tagged using silicon barcodes. Researchers are now working to perfect the system and soon will test it with human oocytes and embryos.

The research, published online in Human Reproduction, represents a first step towards designing a direct labelling system of oocytes and embryos. The objective was to develop a system which minimises risks when identifying female gametes and embryos during in vitro fertilisation and embryo transfer procedures, to reduce the phases of the clinical process requiring control and supervision by two embryologists.

Microscopic silicon codes, fabricated using microelectronic techniques, were employed in the research. In previous tests, researchers verified the innocuousness of silicon particles in human cells, particularly in macrophages. In the present study, the codes were microinjected into the perivitelline space of mouse embryos, located between the cell membrane and the zona pellucida, a cover which surrounds the plasma membrane of the embryo. Since the embryo exits the zona pellucida before its implantation in the uterus, this approximation should allow the embryo to free itself of the identification codes when leaving the zona pellucida.

This research shows that labelled embryos develop normally in culture up to the blastocyst stage, the phase of development which precedes implantation. Researchers also studied the retention of the codes throughout the culture process, the easiness in reading the codes in a standard microscope, and their elimination when embryos free themselves from the zona pellucida. The research also verified the efficacy of the system when freezing and thawing the embryos.

To make the system more viable, researchers are now working on improving the embryo's process of freeing itself from the identification code. This is the only stage of the research which presented limitations. They are currently studying whether the modification of the codes' surface could allow their direct attachment to the outer side of the zona pellucida, avoiding their microinjection into the perivitelline space. They also aim to develop an automatic code reading system.

Researchers recently received authorisation from the Department of Health of the Government of Catalonia to begin testing the system with human oocytes and embryos from several fertility clinics in Spain.


'/>"/>

Contact: Maria Jesus Delgado
MariaJesus.Delgado@uab.cat
34-935-814-049
Universitat Autonoma de Barcelona
Source:Eurekalert

Related biology news :

1. Researchers learn that genetics determine winter vitamin D status
2. Researchers link an African lizard fossil in Africa with the Komodo dragon in Indonesia
3. FSU researchers helping K-12 teachers bring science down to Earth
4. As Arctic temperatures rise, tundra fires increase, researchers find
5. Researchers discover potential genetic target for heart disease
6. Researchers link cerebral malaria to epilepsy, behavior disorders
7. Researchers grow Rett syndrome in a Petri dish
8. U of A researchers can predict heart transplant patients health earlier
9. UCLA researchers discover a potential target for therapy for patients with a deadly prostate cancer
10. Jefferson researchers receive W.W. Smith Charitable Trust
11. Studying the metabolome of smokers, Lombardi researchers find early signs of damage
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/27/2016)... 27, 2016  Rite Track, Inc. a leading semiconductor ... West Chester, Ohio announced today the acquisition ... based in Austin, Texas , will ... provide modifications, installations and technical support offerings for TEL ... PLUS, commented, "PLUS has provided world class service including ...
(Date:1/25/2016)... SEATTLE , Jan. 25, 2016  Glencoe Software, ... biotech, pharma and publication industries, will provide the data ... Phenotypic Screening Centre (NPSC). ... Phenotypic analysis ... even whole organisms, allowing comparisons between states such as ...
(Date:1/22/2016)... DUBLIN , Jan. 22, 2016 /PRNewswire/ ... announced the addition of the "Global ... report to their offering. --> ... of the "Global Biometrics Market in ... offering. --> Research and ...
Breaking Biology News(10 mins):
(Date:2/8/2016)... , Feb. 8, 2016  NanoViricides, Inc. (NYSE MKT: NNVC) (the ... Eugene Seymour , MD, MPH, will present information about the ... Waldorf-Astoria Hotel in New York City . ... in the Windsor Room at 5:30PM EST. Registered attendees can request ... New York City . --> New ...
(Date:2/6/2016)... Roanoke, Virginia (PRWEB) , ... February 06, 2016 ... ... 236, E-mail: amitchell(at)cee(dot)org , The Center for Excellence in Education Sponsors Teacher ... McLean, VA – February 4, 2016 – The Center for Excellence in Education ...
(Date:2/5/2016)... 2016 Australian-US drug discovery and development company, Novogen ... of a new Chairman, Mr John O,Connor , and ... James Garner , has also been formally ... Acting CEO, Mr Iain Ross , will resume his ... James Garner , has also been formally appointed to ...
(Date:2/4/2016)... ... February 04, 2016 , ... ... Digital Forensics Club, takes place February 5-6 at the University’s student center, ... and activities such as workshops and competitions for ample networking, learning and ...
Breaking Biology Technology: