Researchers gain insight into mechanism underlying Hu...( LEXINGTON Ky. (July 13 2009) Resear...)

LEXINGTON, Ky. (July 13, 2009) Researchers at the University of Kentucky Markey Cancer Center and Graduate Center for Toxicology (GCT) have gained new insight into the genetic mechanisms underlying Huntington's disease and other neurodegenerative or neuromuscular disorders caused by trinucleotide repeats (or TNRs) in DNA.

The research, performed in the laboratory of Dr. Guo-Min Li, UK professor of toxicology and biochemistry and the Madeline James & Edith Gardner Distinguished Chair in Cancer Research, examined the mechanisms involved in the development of a specific type of genetic mutation known as trinucleotide repeat expansions. Diseases associated with these mutations, including Huntington's disease, are called trinucleotide repeat disorders.

Findings were published today in Nature Structural & Molecular Biology (http://www.nature.com/nsmb/index.html). GCT research scientist Caixia Hou, student Nelson Chan, and professor Liya Gu are coauthors of the study.

"Mutations the genetic changes in DNA can lead to many different types of disease, depending on where and in what manner they occur," Li said. "How these genetic changes escape normal DNA repair systems and become ingrained in an affected gene pool leading to familial disorders has been a longstanding subject of study in my laboratory at the UK Medical Center."

The expansion of TNRs at unique sites in the human genome is associated with at least 15 familial, neurodegenerative or neuromuscular disorders. The mechanism of TNR instability is poorly understood. However, because DNA expansions require DNA synthesis, DNA replication and/or DNA repair must be involved.

Two key TNRs, CAG and CTG repeats associated with Huntington's disease and myotonic dystrophy, respectively tend to form hairpin structures via strand slippage in the newly synthesized or "nicked" DNA strand during DNA synthesis associated
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