Navigation Links
Researchers find a new gene expression mechanism of PRRS virus
Date:5/12/2014

MANHATTAN, Kan. A collaborative study involving Kansas State University researchers has discovered a new gene expression mechanism in porcine reproductive and respiratory syndrome, or PRRS, virus an important swine pathogen that costs the U.S. pork industry more than $600 million a year. The discovery provides a new avenue for scientists to explore strategies to control and prevent the disease.

Ying Fang, Ph.D., associate professor of diagnostic medicine and pathobiology at Kansas State University, led a study that looked at the unique gene expression mechanism of the PRRS virus. She and colleagues found a new protein in the virus, nsp2TF, was generated through novel ribosomal frameshifting signals.

The research recently appeared in the Proceedings of the National Academy of Sciences, or PNAS, study, "Transactivation of programmed ribosomal frameshifting by a viral protein."

Fang conducted this study with her European collaborators, including Eric Snijder and his team members at Leiden University Medical Center in The Netherlands, and Andrew Firth, Ian Brierley and Brierley's lab members at the University of Cambridge. Yanhua Li, Fang's doctoral student in pathobiology, China, made important contributions to this study. Zhi Sun, Fang's former doctoral student, and Longchao Zhu, visiting scholars in diagnostic medicine and pathobiology in Fang's lab, also were involved in the study.

The study builds on a 2012 PNAS study Fang and her European collaborators conducted while she was at South Dakota State University. In it, researchers identified the nsp2TF protein in the PRRS virus. The protein is expressed through a new gene expression mechanism called -2 ribosomal frameshifting.

"Frameshifting occurs when a ribosome encounters a 'slippery' sequence and downstream signal in messenger RNA," Fang said. "This causes the ribosome to shift two nucleotides backward, which results in all the genetic codons downstream of the shifted site to be read differently and produce a new protein that has a different function."

With the most recent study, Fang and colleagues have shown that this -2 frameshifting requires a PRRS virus protein, nsp1beta. It is the first time a virus's genetic mechanism has been found to require the action of a transacting viral protein rather than a RNA structure to induce a ribosomal frameshifting, which is novel in the protein translation field.

The function of the nsp2TF protein is currently under investigation, Fang said. The protein contains a genetic element that may be responsible for suppressing the pig's immune system.

The newly identified ribosomal frameshifting mechanism may provide an additional antiviral target. Fang's research lab cloned the PRRS virus and then genetically engineered nsp2TF protein knockout viruses.

"These knockout viruses could be potentially used to develop vaccines," Fang said. "Additionally, this novel mechanism of gene expression may also be used by other viruses or in cellular gene expression."

Fang joined Kansas State University in 2013.

"Dr. Fang is recognized as one of the top PRRS experts in the world," said M.M. Chengappa, university distinguished professor of microbiology and head of the department of diagnostic medicine and pathobiology. "We are so fortunate to have faculty of her caliber and stature at Kansas State University. She brings enormous depth and breadth to our research and graduate training program."


'/>"/>

Contact: Ying Fang
yfang@k-state.edu
785-532-4452
Kansas State University
Source:Eurekalert  

Related biology news :

1. Study by UC Santa Barbara researchers suggests that bacteria communicate by touch
2. UC Santa Barbara researchers discover genetic link between visual pathways of hydras and humans
3. Researchers attempt to solve problems of antibiotic resistance and bee deaths in one
4. UNH researchers find African farmers need better climate change data to improve farming practices
5. Ottawa researchers to lead world-first clinical trial of stem cell therapy for septic shock
6. Researchers uncover molecular pathway through which common yeast becomes fungal pathogen
7. Researchers print live cells with a standard inkjet printer
8. Columbia Engineering and Penn researchers increase speed of single-molecule measurements
9. Researchers reveal how a single gene mutation leads to uncontrolled obesity
10. Researchers discover novel therapy for Crohns disease
11. New paper by Notre Dame researchers describes method for cleaning up nuclear waste
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Researchers find a new gene expression mechanism of PRRS virus
(Date:1/30/2017)... --  Invitae Corporation (NYSE: NVTA ), ... announced that it will report its fourth quarter and ... Monday, February 13, 2017, and Invitae,s management team will ... Eastern / 1:45 p.m. Pacific. During ... results, guidance, and recent developments and will spend the ...
(Date:1/24/2017)... Jan. 24, 2017 Biopharm Reports has ... laboratory use of nuclear magnetic resonance spectroscopy (NMR). ... and profiled current practices, developments, trends and end-user ... as growth and opportunities. These areas include growth ... instruments, needs and innovation requirements, hyphenated NMR techniques, ...
(Date:1/19/2017)... PUNE, India , January 19, 2017 ... Sensor Market, Opportunities and Forecast, 2014 - 2022," the global biometric sensor ... of 9.6% from 2016 to 2022. In 2015, Asia-Pacific ... for both public and private sectors. Continue Reading ... ...
Breaking Biology News(10 mins):
(Date:2/17/2017)... , Feb. 17, 2017  If only ... tumor had a mutation-conferring resistance to chemotherapy, thousands ... genomics research has focused on finding these mutations ... even from circulating tumor DNA in blood — ... oncology therapeutics. Unfortunately, however, detecting these ...
(Date:2/16/2017)... , ... February 16, 2017 , ... EIT Digital ... primarily aimed at the agricultural industry. Pilot studies are about to get under way ... IoT, Big Data and 5G innovations. The concept is expected to be transferred eventually ...
(Date:2/16/2017)... MENLO PARK, Calif. and ... -- Longitude Capital, a private investment firm focused on ... today announced the promotion of Josh Richardson ... Richardson focuses on investments in biotechnology companies.  He ... and has played important roles in Longitude,s investments ...
(Date:2/16/2017)... Feb. 16, 2017 Research and ... Biology: Global Markets" report to their offering. ... ... genes, biobrick parts, delivery plasmids, chassis organisms, synthetic cells, ... assembly, genome editing, bioinformatics and specialty media) and enabled ...
Breaking Biology Technology: