Navigation Links
Researchers find a missing component in effort to create primitive, synthetic cells
Date:11/28/2013

drive nonenzymatic replication of RNA, which many scientists believe was the first nucleic acid to develop.

To address the incompatibility between the need for Mg2+ to drive assembly of the RNA molecule and the ion's ability to degrade fatty acid membranes, they tested several chelators small molecules that bind tightly to metal ions for their ability to protect fatty acid vesicles from the potentially destabilizing effects of Mg2+. Citrate and several other chelators were found to be effective in protecting the membranes of fatty acid vesicles from disruption.

To test whether the presence of the tested chelators would allow Mg2+-catalyzed RNA assembly, the investigators placed molecules consisting of short primer RNA strands bound to longer RNA templates into fatty acid vesicles. The unbound, single-strand portion of the template consisted of a sequence of cytosine (C) nucleotides. In the presence of Mg2+ and one of four chelating molecules, one of which was citrate, the researchers then added activated G, the nucleotide that base-pairs with C in nucleic acids.

The desired reaction diffusion of G nucleotides through the vesicle membrane to complete a double-stranded RNA molecule by binding to the C nucleotides of the template proceeded fastest in the presence of citrate. In fact two of the other tested chelators completely prevented extension of the RNA primer.

"While other molecules can protect membranes against the magnesium ion," Szostak explains, "they don't let RNA chemistry go on. We think that citrate is able both to protect membranes and to allow RNA copying to proceed by covering only one face to the magnesium ion, protecting the membrane while allowing RNA chemistry to work." He and Adamala also found that continually refreshing the activated guanine nucleotide solution by flushing out broken down molecules and adding fresh nucleotides improved the efficiency of RNA replication.

Szostak notes
'/>"/>

Contact: Sue McGreevey
smcgreevey@partners.org
617-724-2764
Massachusetts General Hospital
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Study by UC Santa Barbara researchers suggests that bacteria communicate by touch
2. UC Santa Barbara researchers discover genetic link between visual pathways of hydras and humans
3. Researchers attempt to solve problems of antibiotic resistance and bee deaths in one
4. UNH researchers find African farmers need better climate change data to improve farming practices
5. Ottawa researchers to lead world-first clinical trial of stem cell therapy for septic shock
6. Researchers uncover molecular pathway through which common yeast becomes fungal pathogen
7. Researchers print live cells with a standard inkjet printer
8. Columbia Engineering and Penn researchers increase speed of single-molecule measurements
9. Researchers reveal how a single gene mutation leads to uncontrolled obesity
10. Researchers discover novel therapy for Crohns disease
11. New paper by Notre Dame researchers describes method for cleaning up nuclear waste
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/19/2014)... --Research and Markets ( http://www.researchandmarkets.com/research/86ncd6/micro_market ) has ... North America Perimeter Security Systems Market" report to ... The North American perimeter security market is ... 2014 to 2019. Although the U.S. market holds a ... is expected to grow at a higher CAGR ...
(Date:12/17/2014)... 15, 2014 Research and Markets ... the "Samsung Galaxy S5 - Home Button ... their offering. http://photos.prnewswire.com/prnh/20130307/600769 ... sensing technology than the iPhone 5S, Samsung introduces ... its product. The Galaxy S5 home ...
(Date:12/17/2014)... Dec. 16, 2014 Valencell, a leader in ... biometric technology to industry leaders such as Intel, Jabra, ... validated, biometric wearable products. These products will be showcased ... Las Vegas . "Our ... flexible and robust – with the clinical data to ...
Breaking Biology News(10 mins):Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 2Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 3Samsung Galaxy S5 - Home Button Synaptics Fingerprint Sensor Technology Analysis 2Valencell PerformTek Biometrics Power the Most Accurate Wearables at CES 2015 2
... comprehensive framework to encourage and facilitate the sharing of biodiversity ... 24 recommendations of the GBIF Data Publishing Framework Task Group ... Bioinformatics . Some of the measures proposed by ... and others, including the publication of ,data papers, to ...
... from Montana State University,s Museum of the Rockies has revealed how ... famous killer claws, leading to a new hypothesis on the evolution ... in PLoS ONE, MSU researchers Denver W. Fowler, Elizabeth ... Brown University in Rhode Island), describe how comparing modern birds of ...
... -- Cold-weather garden enthusiasts have a new reason to ... University of Ohio have introduced an innovative, all-natural foliar ... enhancing their natural "anti-freeze" properties. According to the scientists, ... location 200 miles souththe equivalent of about one-half of ...
Cached Biology News:New biodiversity data publishing framework proposed 2New biodiversity data publishing framework proposed 3Dinosaurs with killer claws yield new theory about flight 2Dinosaurs with killer claws yield new theory about flight 3Dinosaurs with killer claws yield new theory about flight 4New eco-friendly foliar spray provides natural anti-freeze 2
(Date:12/22/2014)... Dec. 22, 2014  ( www.competitivehealth.com ) — Competitive ... medical bill review and advocacy service, has signed an ... WellCard Savings discount health services marketplace. 63% ... more than they expected to pay. As part of ... costs, WellCard Savings is pleased to offer medical bill ...
(Date:12/22/2014)... 22, 2014 Fertility Associates of Memphis ... Bank USA , a network of more than 20 ... partnership, patients have fast access to a national pool ... IVF . Fertility Associates of Memphis is the only ... treatment option. , Dr. William Kutteh , director ...
(Date:12/19/2014)... BioPlus Specialty Pharmacy (BioPlus), one ... of Nick Maroulis, Pharm.D. to the newly created position ... In this position, Dr. Maroulis will continue to manage ... of our multi-site pharmacies as the company expands. Dr. ... that time he has served in many different capacities ...
(Date:12/19/2014)... Dec. 19, 2014 Bina Technologies, Inc. (Bina, ... that they have been acquired by Roche (SIX: RO, ... privately held company that provides a big data platform ... (NGS) data for the academic and translational research markets. ... and will continue to focus on development of their ...
Breaking Biology Technology:WellCard Savings Announces CoPatient Medical Bill Review Service for Members 2Fertility Associates of Memphis Announces Partnership with Donor Egg Bank USA 2Fertility Associates of Memphis Announces Partnership with Donor Egg Bank USA 3BioPlus Specialty Pharmacy Promotes Dr. Nick Maroulis to Vice President of Specialty Pharmacy Services 2Bina Technologies is acquired by Roche. 2Bina Technologies is acquired by Roche. 3
... ... “Cool” Approach ,to San Antonio and South Central Texas, , ... (PRWEB) June 15, 2010 -- For those looking for a ... offers the Zeltiq ™ Procedure, a new non-invasive body contouring procedure, at his practice ...
... CARLSBAD, Calif. , June 15 Isis Pharmaceuticals, Inc. (Nasdaq: ... of this series of conference calls focused on the drugs in Isis, development ... and will be held on Thursday, June 17, 2010 at ... on Isis, Web site. , , ...
... ... SaaS application for the life sciences industry that incorporates distinctive features and functionality ... ... solutions for the life sciences industry, today announced the release of Version 2.0 ...
Cached Biology Technology:Dr. Suresh Koneru Introduces Zeltiq Non-Invasive Procedure For Fat Reduction 2Dr. Suresh Koneru Introduces Zeltiq Non-Invasive Procedure For Fat Reduction 3Isis Pharmaceuticals to Discuss Its Neurodegenerative Franchise in a Conference Call on Thursday, June 17 2Isis Pharmaceuticals to Discuss Its Neurodegenerative Franchise in a Conference Call on Thursday, June 17 3Viscira Announces Version 2.0 of its Online Interactive Slide Kit Builder Application 2Viscira Announces Version 2.0 of its Online Interactive Slide Kit Builder Application 3
Neutrophil cytosol factor 2 (NCF-2) (Neutrophil NADPH oxidase factor 2) (67 kDa neutrophil oxidase factor) (p67-phox) (NOXA2). [Source:Uniprot/SWISSPROT;Acc:P19878] Antigen: Recombinant Protein E...
... ( Abpromise for all tested applications). ... derived from within residues 2000 to the ... amino acid sequence is proprietary) ... Entrez GeneID: 57697 ...
Rabbit polyclonal to 13,14 dihydro 15 keto Prostaglandin E2 ( Abpromise for all tested applications)....
Arginine vasopressin receptor (AVP-V2), rat. The peptide used for immunization has no significant similarity with AVP V1a or V1b receptors....
Biology Products: