Navigation Links
Researchers find a better way to track stem cells

Tampa, Fla. (April 5, 2010) A study published in the current issue of Cell Transplantation (19:1) has found that using the FDA-approved contrast agent Indocyanine Green (ICG) to label human embryonic stem cell-derived cardiomyocytes (hESC-CMs) substantially improved efforts to optically track stem cells after transplanting them into heart tissues.

The study, carried out by the Departments of Radiology at the University of California and the Technical University of Munich, is freely available on-line at ).

"We found that hESC-CMs labeled with ICG show a significant fluorescence up to 48 hours and can be visualized with optical imaging," said study lead author Dr. Sophie E. Boddington of the UCSF Department of Radiology. "Once more, the labeling procedure did not impair the viability or functional integrity of the cells."

According to the researchers, finding an efficient stem cell delivery technique has been a challenge. Too often, stem cells aimed at myocardial regeneration are "washed away" into noncardiac tissues or they soon die. A 10 percent survival rate for hESC-CMs has been common. Researchers knew that a better way to visualize the transplanted stem cells was needed to help direct and order stem cell delivery, and they also knew that materials used to label the cells had to be maximally fluorescent but not detrimental to the viability of the cells.

"We found the ICG-labeling technique advantageous because it is cost effective, straightforward and rapid," concluded Dr. Boddington. "Its most noticeable advantage is that it can be used with iron oxide-based nanoparticles for use with MRIs, as it increases single cell sensitivity. This is important because of the unpredictable migrational dynamics of the cells."

The researchers added that although PET techniques are more single cell sensitive than MRI, PET introduces radiation exposure and associated risks. In addition to not involving ionized radiation, optical imaging, using the MRI is quicker, less expensive, easier to perform, and noninvasive. Once more, ICG marking is already FDA-approved and accessible.

An added benefit, said Dr. Boddington, is that ICG is rapidly excreted, which suggests safe elimination.

While the ability of hESCs to regenerate into cells of almost all human tissues has been documented, and this provides a promising therapeutic source for myocardial regeneration after cardiac ischema, few studies have actually demonstrated this potential. Visualizing transplanted stem cells could, however, benefit cell retention and improve cell functional outcomes.

"Our data suggests that hESCs labeled with ICG may be useful in assessing delivery routes to improve the engraftment of transplanted hESC-CMs or other stem cell progenitors," concluded Dr. Boddington.

The study has significance, said Dr. Dwaine Emerich, section editor for Cell Transplantation.

"These studies are an important step forward in the quest for techniques to label and visualize stem cells in vivo," said Dr. Emerich. "The ICG-labeling technique described by Boddington and colleagues provides several advantages including its straight forward simplicity, reliability, and the fact that the technique can be easily combined with other developing imaging technologies."


Contact: David Eve
Cell Transplantation Center of Excellence for Aging and Brain Repair

Related biology news :

1. Researchers show some cells in pancreas can spontaneously change into insulin-producing cells
2. UMMS researchers uncover novel genetic pathway responsible for triggering vascular growth
3. Longer-lasting flowers: Fresh ideas from ARS researchers
4. Researchers discover weak link in Alzheimers drug candidates
5. Researchers sequence DNA of peach tree at Clemson University
6. Researchers develop new method to detect melamine in milk
7. Researchers sequence DNA if peach tree at Clemson University
8. New brain nerve cells key to stress resilience, UT Southwestern researchers find
9. Researchers harness the power of plants to fight hemophilia
10. U of I researchers say foliar fungicides may not be the answer for hail-damaged corn
11. Researchers look at reducing yield loss for crops under stress
Post Your Comments:
(Date:11/10/2015)... LONDON , Nov. 10, 2015 /PRNewswire/ ... segmented on the basis of product, type, ... segments included in this report are consumables, ... this report are safety biomarkers, efficacy biomarkers, ... in this report are diagnostics development, drug ...
(Date:11/9/2015)... , Nov. 9, 2015  Synaptics Inc. (NASDAQ: ... solutions, today announced broader entry into the automotive market ... that match the pace of consumer electronics human interface ... sensors are ideal for the automotive industry and will ... Europe , Japan ...
(Date:10/29/2015)... MINNETONKA, Minn. , Oct. 29, 2015   ... that supports the entire spectrum of clinical research, is ... the Minnesota High Tech Association (MHTA) as one of ... in the "Software – Small and Growing" category. The ... and individuals who have shown superior technology innovation and ...
Breaking Biology News(10 mins):
(Date:11/24/2015)... , Nov. 24, 2015 Halozyme Therapeutics, Inc. (NASDAQ: ... in New York on Wednesday, December 2 ... Torley , president and CEO, will provide a corporate overview. ... York at 1:00 p.m. ET/10:00 a.m. PT . ... relations, will provide a corporate overview. --> th ...
(Date:11/24/2015)... Inc., a worldwide provider of clinical research services headquartered in ... has set a new quarterly earnings record in Q3 of 2015.  ... Q3 of 2014 to Q3 of 2015.   ... the establishment of an Asia-Pacific office to ... and Mexico , with the establishment ...
(Date:11/24/2015)... 24, 2015 --> ... report released by Transparency Market Research, the global non-invasive ... CAGR of 17.5% during the period between 2014 and ... Global Industry Analysis, Size, Volume, Share, Growth, Trends and ... testing market to reach a valuation of US$2.38 bn ...
(Date:11/24/2015)... ... , ... InSphero AG, the leading supplier of easy-to-use solutions for production, culture, ... serve as Chief Operating Officer. , Having joined InSphero in November 2013 ... was promoted to Head of InSphero Diagnostics in 2014. There she has built ...
Breaking Biology Technology: