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Researchers expand efforts to explore functional landscape of the human genome
Date:10/9/2007

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  • Gregory Crawford, Ph.D.; Duke University Institute for Genome Sciences & Policy, Durham, N.C.; $6.5 million (four years); Comprehensive Identification of Active Functional Elements in Human Chromatin. These researchers will seek to identify and characterize regions of open chromatin through DNase I hypersensitivity assays, formaldehyde-assisted isolation of regulatory elements and chromatin immunoprecipitation for a few key DNA-binding factors. Chromatin is the complex of DNA and proteins that makes up chromosomes.

  • Thomas Gingeras, Ph.D.; Affymetrix Inc., Santa Clara, Calif.; $10.2 million (four years); Comprehensive Characterization and Classification of the Human Transcriptome. This group will identify protein-coding and non-protein-coding ribonucleic acid (RNA) transcripts using microarrays, high-throughput sequencing, sequenced paired-end ditags and sequenced cap analysis of gene expression tags. RNA is an information molecule vital to a number of biological functions, including protein production.

  • Tim Hubbard, Ph.D.; Wellcome Trust Sanger Institute, Hinxton, England; $8.5 million (four years); Integrated Human Genome Annotation: Generation of a Reference Gene Set. Using computational methods, manual annotation and targeted experiments, this team will annotate gene features in the human genome. Such features include genes that code for proteins; genes that are transcribed, but do not code for proteins; and pseudogenes, which are DNA sequences similar to normal genes, but which have been altered slightly so they are not functional.

  • Richard Myers, Ph.D.; Stanford University, Stanford, Calif.; $14.6 million (four years); Global Annotation of Regulatory Elements in the Human Genome. This group has two goals: to identify transcription factor binding sites by using chromatin immunoprecipitation followed by high-throughput sequencing, and to pilot the use of high-throughpu
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Contact: Geoff Spencer
spencerg@mail.nih.gov
301-402-0911
NIH/National Human Genome Research Institute
Source:Eurekalert

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