Navigation Links
Researchers divide enzyme to conquer genetic puzzle
Date:3/14/2013

alves of the split RNAP were present. Bennett and Shis modified the bacterium E. coli to produce the RNAP segments in response to specific sugar molecules found in the environment. One half of the RNAP is produced only in the presence of arabinose and the other in the presence of lactose. When both sugars were found, their proof-of-concept polymerase would turn on a target gene, in this case a reporter gene that encodes a green fluorescent protein.

Better yet, the RNAP was orthogonal; that is, it didn't fraternize with the E. coli's native protein pathways. "This special T7 RNAP will not turn on any other gene but its specific target," Bennett said. "In that way, it's transparent to the host. This gives us an easy way to determine whether or not it's working."

The researchers found that while split T7 RNAP was not as active in expressing protein products as the full-length polymerase, the pieces were more stable and less prone to mutations that could affect a gene circuit's function.

Bennett said the next step would be to test the split RNAP in hosts other than E. coli. "We want to port this into more complicated organisms: eukaryotes like yeast or zebrafish or mammalian cells. It will take a little bit of engineering to get them to work in more complicated organisms."

He sees advanced diagnostics as a reasonable goal for gene circuits using AND gates. "The two inputs can be programmed to respond to separate conditions, whether they're environment factors outside the organism or tissue-specific markers within a multicellular organism," Bennett said.

"For instance, your inputs could be cancer markers that would trigger a fluorescent reporter gene for diagnosis or tumor suppressors for treatment," he said. In addition, multiple gates from the library could be combined and layered to create more complicated circuits that simultaneously monitor many variables. "This means you could build circuits that turn on only in ve
'/>"/>

Contact: Jeff Falk
jfalk@rice.edu
713-348-6775
Rice University
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Study by UC Santa Barbara researchers suggests that bacteria communicate by touch
2. UC Santa Barbara researchers discover genetic link between visual pathways of hydras and humans
3. Researchers attempt to solve problems of antibiotic resistance and bee deaths in one
4. UNH researchers find African farmers need better climate change data to improve farming practices
5. Ottawa researchers to lead world-first clinical trial of stem cell therapy for septic shock
6. Researchers uncover molecular pathway through which common yeast becomes fungal pathogen
7. Researchers print live cells with a standard inkjet printer
8. Columbia Engineering and Penn researchers increase speed of single-molecule measurements
9. Researchers reveal how a single gene mutation leads to uncontrolled obesity
10. Researchers discover novel therapy for Crohns disease
11. New paper by Notre Dame researchers describes method for cleaning up nuclear waste
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/30/2014)... you want your doctor to know what goes wrong with ... good idea to know what "normal" actually is. That,s where ... of the FASEB Journal comes in. ... transcriptomea key set of molecules that can help scientists "see" ... time. What,s more, they found never-before-detected gene activity and that ...
(Date:9/30/2014)... threats in today,s ocean. From overfishing and pollution ... ecosystems are disappearing at unprecedented rates around the ... surrounding the island of Moorea in French Polynesia ... coral guard-crabs. New research from the National Museum ... McKeon and the museum,s predoctoral fellow Jenna Moore ...
(Date:9/29/2014)... that the exposure to tar tended to be lower ... Similarly, exposure to nicotine tended to be lower. ... cigarette in several countries around the world. Previous studies ... in the smoke of these cigarettes are lower than ... chemicals in the smoke are not necessarily linked to ...
Breaking Biology News(10 mins):Scientists identify which genes are active in muscles of men and women 2Smithsonian scientists discover coral's best defender against an army of sea stars 2Slim cigarette smokers not exposed to more harmful chemicals 2
... of Salamanca and another from Yale University have shown that ... beershebensis , a lizard species from the Negev Desert in ... less and catch less mobile and different prey if they ... predicted this result, but until now there had been very ...
... National Primate Research Center, Emory University, have successfully ... step toward unlocking the genetic secrets of pair ... will enable scientists to perform a host of ... mechanisms of social bonding and other complex social ...
... is available in German . , Brown adipose ... mitochondria, miniature power stations which among other things can ,burn, ... that of a battery, which then provides energy for cellular processes. ... They go full steam ahead all the time. The energy released ...
Cached Biology News:Lizards change their diet to avoid predators 2Yerkes researchers create first transgenic prairie voles 2Brown fat cells make 'spare tires' shrink 2
(Date:9/30/2014)... September 30, 2014 Lyme Research ... of Lyme disease research at universities, today announced the ... on the identification, treatment and cure for Lyme and ... received the most applications in its history—over 20 grant ... “While we were pleased to receive so many solid ...
(Date:9/30/2014)... September 30, 2014 On September 11, Pharma ... Sherley in anticipation of a session that he will lead ... Summit,” scheduled for February 17-18, 2015 in London. In ... IQ asked Dr. Sherley to respond to several questions on ... challenges to evaluating stem cell toxicity, and future trends in ...
(Date:9/30/2014)... Ontario, Canada (PRWEB) September 30, 2014 Back ... based on 500 square feet. per employee, which included space ... world back then.” says Jeff Howell, partner at Nidea ... advisory firm in Toronto. “By the year 2000, however, ... are now seeing that number come down to 175 to ...
(Date:9/29/2014)... at the forefront of diagnostics today, with imaging ... tomography), scanning, and NMR (nuclear magnetic resonance) increasing ... problems of image resolution and quality still limit ... tissue. A solution is hyperpolarization, which involves injecting ... quality by following the distribution and fate of ...
Breaking Biology Technology:Lyme Research Alliance Awards Nearly $600,000 in New Grants 2Lyme Research Alliance Awards Nearly $600,000 in New Grants 3Lyme Research Alliance Awards Nearly $600,000 in New Grants 4Adult Stem Cell Technology Center, LLC Director James L. Sherley Discusses Stem Cell Toxicology 2Adult Stem Cell Technology Center, LLC Director James L. Sherley Discusses Stem Cell Toxicology 3Adult Stem Cell Technology Center, LLC Director James L. Sherley Discusses Stem Cell Toxicology 4ITRA Global Reports on How Companies Are Squeezing More Employees into Less Office Space 2ITRA Global Reports on How Companies Are Squeezing More Employees into Less Office Space 3A safer approach for diagnostic medical imaging 2A safer approach for diagnostic medical imaging 3
... 2011 Clinicians now have a new tool to help ... -- one of the most common sexually transmitted infections in ... BD (Becton, Dickinson and Company), announced today that it received ... first fully automated molecular tests to detect and differentiate HSV ...
... 2011 The Japanese nuclear crisis has caused a ... not only in Japan, but as far away as ... iodide does not provide generalized radiation protection because it ... bombarded with orders as the radiation hysteria mounts. ...
... (Nasdaq: VRML ) – Vermillion, a molecular ... coverage March 23, 2011 on Vermillion. This ... at Stephens by contacting, Scott Gleason at scott.gleason@stephens.com ... Vermillion, Inc. is dedicated to the discovery, development and ...
Cached Biology Technology:BD Diagnostics Receives FDA Clearance for First Fully Automated Molecular Assays for HSV1 and HSV2 from Clinician-Collected External Anogenital Lesion Specimens 2Shortages of Potassium Iodide as Radiation Panic Drives Demand 2Stephens Initiates Coverage on Vermillion 2Stephens Initiates Coverage on Vermillion 3
... Motic's B3 series of microscopes offer ... microscope required for laboratory work. Equipped with ... Super Contrast (ASC) objectives. The B3 is ... The Siedentopf binocular tube eliminates the need ...
... SYBR Green II RNA gel ... gel stain that has bright fluorescence when ... gels, making it ideal for use with ... scanners or standard UV transilluminators. A 500 ...
... SYBR Green II RNA gel stain ... has bright fluorescence when bound to RNA and ... use with either formaldehyde/agarose or polyacrylamide gels using ... stains 100 minigels. A 500 µL unit size ...
... Glutathion-S-transferases (GST) are a group ... the detoxification processes. The GSTs are ... Pi, Teta and microsomal. The classes ... and s Sample Size: ...
Biology Products: