Navigation Links
Researchers divide enzyme to conquer genetic puzzle
Date:3/14/2013

Rice University researchers have found a way to divide and modify enzymes to create what amounts to a genetic logic gate.

Biochemist Matthew Bennett and graduate student David Shis created a library of AND gates by mutating a protein from a bacterial virus. The well-understood protein known as T7 RNA polymerase (RNAP) is a strong driver of transcription in cells.

Their discovery should help overcome a bottleneck in the development of synthetic gene networks that mimic digital circuitry. These networks could become diagnostic systems that look for signs of disease and, perhaps, gene therapies to find and treat disease in one step.

The research appeared online this week in the Proceedings of the National Academy of Sciences.

"AND logic gates are normally found in electronics: You have a circuit with two inputs and one output," said Bennett, an assistant professor of biochemistry and cell biology. "In an AND gate, if the two wires leading to the gate are both on, then the output is also on. If either one or both are off, then the output is turned off."

Few options have been available to researchers seeking reliable and flexible components for their synthetic circuits. The library of AND gates created at Rice should add significantly to the toolbox available to build larger and more complex gene circuits, Bennett said.

In its native, full-length state, T7 RNAP turns on genes that have a specific "promoter," or target DNA sequence. The Rice researchers found they could program DNA to express the RNAP in two pieces, which could be manipulated via point mutations to target different promoters in a host cell. "The two pieces of the RNAP might even be made in different parts of the cell and they'll still find each other," Bennett said. "They have an affinity for each other, and once they combine, they'll work together as if they hadn't been split."

The enzyme carried out its function only when both h
'/>"/>

Contact: Jeff Falk
jfalk@rice.edu
713-348-6775
Rice University
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Study by UC Santa Barbara researchers suggests that bacteria communicate by touch
2. UC Santa Barbara researchers discover genetic link between visual pathways of hydras and humans
3. Researchers attempt to solve problems of antibiotic resistance and bee deaths in one
4. UNH researchers find African farmers need better climate change data to improve farming practices
5. Ottawa researchers to lead world-first clinical trial of stem cell therapy for septic shock
6. Researchers uncover molecular pathway through which common yeast becomes fungal pathogen
7. Researchers print live cells with a standard inkjet printer
8. Columbia Engineering and Penn researchers increase speed of single-molecule measurements
9. Researchers reveal how a single gene mutation leads to uncontrolled obesity
10. Researchers discover novel therapy for Crohns disease
11. New paper by Notre Dame researchers describes method for cleaning up nuclear waste
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:11/5/2014)... Brazil -Individuals show great diversity in their ability to ... females greatly differ in their perceptual evaluation of odors, ... tests. , Sex differences in olfactory detection ... may be connected to one,s perception of smell, which ... women,s olfactory superiority has been suggested to be cognitive ...
(Date:11/4/2014)... Fla. (November 4, 2014) — Think about the way ... How do neighboring cells know that they are supposed ... how do these tissues find the correct place and ... answering these crucial questions. , In a new study, ... to communicate with their surrounding neighbors, at the head-trunk ...
(Date:11/4/2014)... , Nov. 4, 2014   Neurotechnology ... today announced that the latest version of its ... the Ongoing MINEX evaluation organized by ... fingerprint algorithms using the INCITS 378 fingerprint standard ... requirement in public tenders in the ...
Breaking Biology News(10 mins):The female nose always knows: Do women have more olfactory neurons? 2The inside story: How the brain and skull stay together 2Neurotechnology Places Second in Ongoing MINEX Ranking for Fingerprint Matching Algorithms 2Neurotechnology Places Second in Ongoing MINEX Ranking for Fingerprint Matching Algorithms 3
... of adults with autism has provided new evidence that ... may not communicate with each other as efficiently as ... University of Washington's Autism Center will report today at ... the first study that measures neural activity by using ...
... leading African-American lawmakers, civil rights leaders and medical ... adopt and implement a new blueprint to address ... The plan is outlined in a new report, ... the Epidemic in Black America, written by Robert ...
... from traditional radiation and chemotherapy treatment for head and ... good prognosis with treatment of p53 tumor suppressor gene ... test for a gene-based drug. , Researchers at Introgen ... advanced squamous cell carcinoma of the head and neck ...
Cached Biology News:Brain regions do not communicate efficiently in adults with autism 2New HIV statistics indicate increasing toll of AIDS on African American community 2New HIV statistics indicate increasing toll of AIDS on African American community 3New HIV statistics indicate increasing toll of AIDS on African American community 4Abnormal overexpression of p53 is a predictive molecular biomarker 2
(Date:11/22/2014)... RURO, Inc., a leading LIMS, ... Limfinity® version 5.1.4. , RURO’s deployment teams have ... ever in RURO’s 8 year history. Many deployments ... Limfinity® version 5.1.4 is the perfect example – ... fixes! , Limfinity® 5.1.4 release highlights – more ...
(Date:11/22/2014)... , Nov. 21, 2014  The element of ... emerging diseases, i.e., they strike without warning. Until now, ... warning of a viral outbreak, resulting in suboptimal responses ... Ebola currently. Photo - http://photos.prnewswire.com/prnh/20141121/160242 ... has now been removed because virus gene structural changes ...
(Date:11/21/2014)... 2014 The science of light and ... with more than 200 primary students in a traveling ... University of Otago Physics Department and funded by an ... members of the university’s OSA/SPIE Student Chapter visited six ... giving children from small, rural schools hands-on lessons in ...
(Date:11/21/2014)... 2014 The report, "Synthetic ... Enzymes, Cloning kits), Technology (Bioinformatics, Nanotechnology, Gene ... Biomaterials, Bioremediation) - Global Forecast to 2018", ... threats, opportunities, and challenges. , Browse 99 ... through 185 pages and in-depth TOC of ...
Breaking Biology Technology:RURO Announces Limfinity® version 5.1.4 2Viruses' Advantage of Surprise is Lost 2Optics and photonics outreach programs share science, spark fascination with light 2Optics and photonics outreach programs share science, spark fascination with light 3Synthetic Biology Market Expected to Reach $5,630.4 Million in 2018 - New Report by MarketsandMarkets 2Synthetic Biology Market Expected to Reach $5,630.4 Million in 2018 - New Report by MarketsandMarkets 3Synthetic Biology Market Expected to Reach $5,630.4 Million in 2018 - New Report by MarketsandMarkets 4
... interactive gene-level database to explore, understand, manage, and ... GenomeQuest, Inc., the leader in sequence data ... Digital Gene Expression Workflow designed to enable researchers ... Expression Workflow is the second such workflow to ...
... new Hospital Energy Performance Service can ... energy efficiency by 25% or more ... modifications. The service is available from Environmental ... environmental and engineering consulting firm."Healthcare facilities are heavy energy users ...
... 5 - Evoke Pharma, Inc., a privately-held specialty ... Phase 2b clinical trial to evaluate EVK-001 for the ... is a syndrome characterized by delayed emptying of the ... absence of mechanical obstruction. Diabetes is an important cause ...
Cached Biology Technology:GenomeQuest, Inc. Announces Digital Gene Expression Solution Within Its Sequence Data Management Platform 2New Hospital Energy Performance Service Helps Hospitals Cut Energy Costs by 25% or More 2Evoke Pharma, Inc. Initiates Phase 2b Metoclopramide Intranasal Trial (MINT) in Patients With Diabetic Gastroparesis 2Evoke Pharma, Inc. Initiates Phase 2b Metoclopramide Intranasal Trial (MINT) in Patients With Diabetic Gastroparesis 3