Navigation Links
Researchers divide enzyme to conquer genetic puzzle
Date:3/14/2013

Rice University researchers have found a way to divide and modify enzymes to create what amounts to a genetic logic gate.

Biochemist Matthew Bennett and graduate student David Shis created a library of AND gates by mutating a protein from a bacterial virus. The well-understood protein known as T7 RNA polymerase (RNAP) is a strong driver of transcription in cells.

Their discovery should help overcome a bottleneck in the development of synthetic gene networks that mimic digital circuitry. These networks could become diagnostic systems that look for signs of disease and, perhaps, gene therapies to find and treat disease in one step.

The research appeared online this week in the Proceedings of the National Academy of Sciences.

"AND logic gates are normally found in electronics: You have a circuit with two inputs and one output," said Bennett, an assistant professor of biochemistry and cell biology. "In an AND gate, if the two wires leading to the gate are both on, then the output is also on. If either one or both are off, then the output is turned off."

Few options have been available to researchers seeking reliable and flexible components for their synthetic circuits. The library of AND gates created at Rice should add significantly to the toolbox available to build larger and more complex gene circuits, Bennett said.

In its native, full-length state, T7 RNAP turns on genes that have a specific "promoter," or target DNA sequence. The Rice researchers found they could program DNA to express the RNAP in two pieces, which could be manipulated via point mutations to target different promoters in a host cell. "The two pieces of the RNAP might even be made in different parts of the cell and they'll still find each other," Bennett said. "They have an affinity for each other, and once they combine, they'll work together as if they hadn't been split."

The enzyme carried out its function only when both h
'/>"/>

Contact: Jeff Falk
jfalk@rice.edu
713-348-6775
Rice University
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Study by UC Santa Barbara researchers suggests that bacteria communicate by touch
2. UC Santa Barbara researchers discover genetic link between visual pathways of hydras and humans
3. Researchers attempt to solve problems of antibiotic resistance and bee deaths in one
4. UNH researchers find African farmers need better climate change data to improve farming practices
5. Ottawa researchers to lead world-first clinical trial of stem cell therapy for septic shock
6. Researchers uncover molecular pathway through which common yeast becomes fungal pathogen
7. Researchers print live cells with a standard inkjet printer
8. Columbia Engineering and Penn researchers increase speed of single-molecule measurements
9. Researchers reveal how a single gene mutation leads to uncontrolled obesity
10. Researchers discover novel therapy for Crohns disease
11. New paper by Notre Dame researchers describes method for cleaning up nuclear waste
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/21/2015)... According to a new ... Software, Services), by Applications (Surveillance (Airborne, Maritime, Land), ... (Military & Defense & Commercial) - Global Forecast ... is expected to grow from $7252.0 Million in ... Compound Annual Growth Rate (CAGR) of 6.6%. ...
(Date:5/20/2015)... 20, 2015 NXT-ID, Inc. (NASDAQ: NXTD ... owned subsidiary, 3D-ID LLC, a company engaged in biometric ... the biometrics technology portion of the recently awarded TIES ... Department of the Army. 3D-ID LLC has ... government, law enforcement and security agencies. ...
(Date:5/19/2015)... 2015 Research and ... of the  "Genetic Testing Market Outlook 2018" ... , ,A recent report, Genetic Testing Market ... the current and future genetic testing market. ... working principles and types are covered in ...
Breaking Biology News(10 mins):Thermal Imaging Market Worth $9,998.9 Million by 2020 2Thermal Imaging Market Worth $9,998.9 Million by 2020 3Thermal Imaging Market Worth $9,998.9 Million by 2020 4NXT-ID, Inc Announces Participation as Member of Team Battelle for TIES Contract Award 2NXT-ID, Inc Announces Participation as Member of Team Battelle for TIES Contract Award 3Global Genetic Testing Market Outlook 2018 2
... National Zoos Conservation and Research Center in Front Royal, Va., ... not vanish from the planet. The oryx, along ... to Tunisia in December 2007 for an eventual reintroduction into ... 1970s. Oryx are a type of desert antelope that are ...
... more than 10 percent each year from 2010 to ... fossil fuels, but this is an unsustainable prospect. According ... Nuclear Governance, Economy and Ecology such a large growth ... efficiency otherwise each new power plant will simply cannibalize ...
... Solution Improves Officer Situational Awareness, WALL, N.J., ... BKYI), a leader in finger-based biometric,identification and wireless ... contract from the San Diego Harbor Police Department ... location (AVL) capability,in its patrol vehicles. San Diego ...
Cached Biology News:National Zoo scimitar-horned oryx going into the wild 2Nuclear cannibals 2San Diego Harbor Police Deploy BIO-key(R) Automated Vehicle Location System 2San Diego Harbor Police Deploy BIO-key(R) Automated Vehicle Location System 3
(Date:5/21/2015)... 21, 2015 Patent Offering, The patent’s ... diagnostic or therapeutic imaging within a body lumen (open ... endoscope having a low cost, single-use disposable illumination and ... was October 18, 2013 and the patent approval was ... the physician to customize the lighting and magnification of ...
(Date:5/21/2015)... , May 21, 2015 /PRNewswire/ - RepliCel Life ... stage regenerative medicine company focused on the development ... poster presentation at the International Society for Cellular ... chronic Achilles tendinosis currently in a Phase 1/2 ... 29 th from 5:30 PM to 7:00 ...
(Date:5/20/2015)... DUBLIN , May 20, 2015 Research and ... addition of the "Top Technologies in HW_Technical Insights" ... division of this report evaluated technology trends in the ... trends that are likely to have an impact in ... depth analysis of the top 10 health and wellness ...
(Date:5/20/2015)... The global genotyping market is expected to grow at ... 2015 to 2020 to reach $17.0billion in 2020. Genotyping ... development process and to provide personalized therapy, factors that ... by key manufacturers to offer advanced and innovative products ... 113 market data tables & 37 figures spread through ...
Breaking Biology Technology:IpAuctions™ Presents The Worlds Smallest Disposable Illuminated Endoscope For Auction 2IpAuctions™ Presents The Worlds Smallest Disposable Illuminated Endoscope For Auction 3RepliCel to Present Unique Autologous Cell Treatment for Achilles Tendinosis at International Society for Cellular Therapy Conference 2RepliCel to Present Unique Autologous Cell Treatment for Achilles Tendinosis at International Society for Cellular Therapy Conference 3Top Technologies in the Health and Wellness Industry 2015 2Genotyping Market Growing at 22.3% CAGR to 2020: Research Based on Technology, Applications, Opportunities, Challenges and Key Players. 2Genotyping Market Growing at 22.3% CAGR to 2020: Research Based on Technology, Applications, Opportunities, Challenges and Key Players. 3Genotyping Market Growing at 22.3% CAGR to 2020: Research Based on Technology, Applications, Opportunities, Challenges and Key Players. 4Genotyping Market Growing at 22.3% CAGR to 2020: Research Based on Technology, Applications, Opportunities, Challenges and Key Players. 5
... a Madison-based biotechnology company determined to solve the ... $250,000 Technology Development Fund Loan (TDF) from The ... use the low-interest, deferred-payment loan to develop its ... , ,The Technology Development Fund is a spectacular ...
... analysts, consultants and executives are reporting that there are ... second half of 2003, which provides the necessary first ... , ,Steve Haroldson, chief technology officer for CUNA Mutual ... I talk regularly with technology vendors, and over the ...
... CIO magazines CIO Award, given to 100 ... of information technology(IT) within their enterprises. Alliant Energy, Lands ... the award, announced in the August 15 issue of ... the cover of the magazine. , ,The magazine says ...
Cached Biology Technology:IT Spending to Increase in Second Half of 2003 2IT Spending to Increase in Second Half of 2003 3IT Spending to Increase in Second Half of 2003 4IT Spending to Increase in Second Half of 2003 5Five Wisconsin Non-Tech Companies Win IT Awards 2Five Wisconsin Non-Tech Companies Win IT Awards 3Five Wisconsin Non-Tech Companies Win IT Awards 4