Navigation Links
Researchers discover important tool in understanding differentiation in human embryonic stem cells
Date:10/24/2007

Researchers at the University of Minnesotas Stem Cell Institute have described how an existing genetic tool can be used to study how human embryonic stem cells differentiate. The research appears in the November 2007 issue of Experimental Biology and Medicine.

Researchers know very little about how human embryonic stem cells (hESC) self-renew. To fully understand these cells self renewal capacity and pluripotency, and their regulation, it is necessary to efficiently generate genetically modified cells and analyze the consequences of elevated and reduced expression of genes.

The research team, led by the University of Minnesotas Meri Firpo, Ph.D., included gene therapy researchers at Los Angeles Childrens Hospital, and developmental biologists at the University of Michigan.

The researchers used knockdown technology to reduce the expression of oct4, a gene known to be necessary for self renewal of mouse and human embryonic stem cells. As seen in work done with mouse cells by knockdown and other genetic means, they showed that reducing the amount of oct4 in human ES cells induced differentiation. The researchers then used a plasmid vector to transiently increase levels of oct4 in hESC. This also resulted in differentiation as expected, but with differentiation patterns similar to those seen with the knockdown. This was an unexpected result, because when expression of oct4 is up-regulated in mouse ES cells, they differentiate into a different type of cell than if the expression of oct4 is down-regulated.

"This suggests a key difference in the regulation of early development between mouse and human embryos" Firpo said. While animal models are clearly important, this research shows that scientists need human models to truly understand what happens in early human development.


'/>"/>

Contact: Dr. Meri Firpo
firpo001@umn.edu
612-625-3250
Society for Experimental Biology and Medicine
Source:Eurekalert

Page: 1

Related biology news :

1. Researchers discover way to make cells in the eye sensitive to light
2. Researchers find how protein allows insects to detect and respond to pheromones
3. Researchers Uncover Key Step In Manufacture of Memory Protein
4. NYU researchers simulate molecular biological clock
5. Researchers reveal the infectious impact of salmon farms on wild salmon
6. Researchers identify target for cancer drugs
7. Vital step in cellular migration described by UCSD medical researchers
8. ASU researchers finds novel chemistry at work to provide parrots vibrant red colors
9. UCSD researchers maintain stem cells without contaminated animal feeder layers
10. Researchers discover molecule that causes secondary stroke
11. Researchers find missing genes of ancient organism
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/13/2017)... 2017 UBM,s Advanced Design and Manufacturing event ... emerging and evolving technology through its 3D Printing and ... alongside the expo portion of the event and feature ... focused on trending topics within 3D printing and smart ... event will take place June 13-15, 2017 at the Jacob ...
(Date:4/11/2017)... Florida , April 11, 2017 ... a security technology company, announces the appointment of independent Directors ... Bendheim to its Board of Directors, furthering the company,s ... ... of NXT-ID, we look forward to their guidance and benefiting ...
(Date:4/4/2017)... April 4, 2017   EyeLock LLC , a ... the United States Patent and Trademark Office (USPTO) has ... the linking of an iris image with a face ... represents the company,s 45 th issued patent. ... very timely given the multi-modal biometric capabilities that have ...
Breaking Biology News(10 mins):
(Date:10/10/2017)... ... 10, 2017 , ... The Pittcon Program Committee is pleased ... scientists who have made outstanding contributions to analytical chemistry and applied spectroscopy. Each ... leading conference and exposition for laboratory science, which will be held February 26-March ...
(Date:10/9/2017)... ... 2017 , ... The award-winning American Farmer television series will feature 3 Bar ... Tuesdays at 8:30aET on RFD-TV. , With global population estimates nearing ten billion ... continue to feed a growing nation. At the same time, many of our valuable ...
(Date:10/9/2017)... ... 09, 2017 , ... The Giving Tree Wellness Center announces ... needs of consumers who are incorporating medical marijuana into their wellness and health ... As operators of two successful Valley dispensaries, The Giving Tree’s two founders, Lilach ...
(Date:10/7/2017)... , ... October 06, 2017 ... ... experience providing advanced instruments and applications consulting for microscopy and surface analysis, ... in application consulting, Nanoscience Analytical offers a broad range of contract analysis ...
Breaking Biology Technology: