Navigation Links
Researchers discover important tool in understanding differentiation in human embryonic stem cells
Date:10/24/2007

Researchers at the University of Minnesotas Stem Cell Institute have described how an existing genetic tool can be used to study how human embryonic stem cells differentiate. The research appears in the November 2007 issue of Experimental Biology and Medicine.

Researchers know very little about how human embryonic stem cells (hESC) self-renew. To fully understand these cells self renewal capacity and pluripotency, and their regulation, it is necessary to efficiently generate genetically modified cells and analyze the consequences of elevated and reduced expression of genes.

The research team, led by the University of Minnesotas Meri Firpo, Ph.D., included gene therapy researchers at Los Angeles Childrens Hospital, and developmental biologists at the University of Michigan.

The researchers used knockdown technology to reduce the expression of oct4, a gene known to be necessary for self renewal of mouse and human embryonic stem cells. As seen in work done with mouse cells by knockdown and other genetic means, they showed that reducing the amount of oct4 in human ES cells induced differentiation. The researchers then used a plasmid vector to transiently increase levels of oct4 in hESC. This also resulted in differentiation as expected, but with differentiation patterns similar to those seen with the knockdown. This was an unexpected result, because when expression of oct4 is up-regulated in mouse ES cells, they differentiate into a different type of cell than if the expression of oct4 is down-regulated.

"This suggests a key difference in the regulation of early development between mouse and human embryos" Firpo said. While animal models are clearly important, this research shows that scientists need human models to truly understand what happens in early human development.


'/>"/>

Contact: Dr. Meri Firpo
firpo001@umn.edu
612-625-3250
Society for Experimental Biology and Medicine
Source:Eurekalert

Page: 1

Related biology news :

1. Researchers discover way to make cells in the eye sensitive to light
2. Researchers find how protein allows insects to detect and respond to pheromones
3. Researchers Uncover Key Step In Manufacture of Memory Protein
4. NYU researchers simulate molecular biological clock
5. Researchers reveal the infectious impact of salmon farms on wild salmon
6. Researchers identify target for cancer drugs
7. Vital step in cellular migration described by UCSD medical researchers
8. ASU researchers finds novel chemistry at work to provide parrots vibrant red colors
9. UCSD researchers maintain stem cells without contaminated animal feeder layers
10. Researchers discover molecule that causes secondary stroke
11. Researchers find missing genes of ancient organism
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/23/2017)... India , March 23, 2017 The report "Gesture ... Touchless Biometric), Industry, and Geography - Global Forecast to 2022", published by MarketsandMarkets, ... at a CAGR of 29.63% between 2017 and 2022. ... ... ...
(Date:3/22/2017)... YORK , March 21, 2017 ... Marketing Cloud used by retailers such as 1-800-Flowers ... its platform — Product Recommendations and Replenishment. Using Optimove,s ... give more personalized product and replenishment recommendations to ... but also on predictions of customer intent drawn ...
(Date:3/20/2017)... , March 20, 2017 PMD Healthcare ... personal spirometer and Wellness Management System (WMS), a remote, ... Founded in 2010, PMD Healthcare is a Medical Device, ... a mission dedicated to creating innovative solutions that empower ... With that intent focus, PMD developed the first ever ...
Breaking Biology News(10 mins):
(Date:6/16/2017)... CT (PRWEB) , ... June 16, 2017 , ... ... night’s Entrepreneur Innovation Awards (EIA), held at The LOFT at Chelsea Piers in Stamford. ... project ideas to a panel of judges for an opportunity to secure $10,000 awards ...
(Date:6/15/2017)... ... June 15, 2017 , ... The newest exhibition at the ... creative experimentation and interdisciplinary collaboration. Feature Creep, a solo exhibition by Maximillian Lawrence, ... will be held at EKG, located at 3600 Market Street in Philadelphia, on ...
(Date:6/15/2017)... ... June 15, 2017 , ... Adam Equipment, a ... to assemble a lab workstation. The guide outlines the procedure in four simple ... Eclipse balance, AVT anti-vibration table, OIML/ASTM certified weights, and Adam DU specialized software. ...
(Date:6/14/2017)... , ... June 14, 2017 , ... ... materials for pumps and process equipment, has appointed Andrew Ondish as Territory Sales ... Ondish holds a B.S. degree in Petroleum Engineering from Colorado School of Mines. ...
Breaking Biology Technology: