Navigation Links
Researchers discover important tool in understanding differentiation in human embryonic stem cells
Date:10/24/2007

Researchers at the University of Minnesotas Stem Cell Institute have described how an existing genetic tool can be used to study how human embryonic stem cells differentiate. The research appears in the November 2007 issue of Experimental Biology and Medicine.

Researchers know very little about how human embryonic stem cells (hESC) self-renew. To fully understand these cells self renewal capacity and pluripotency, and their regulation, it is necessary to efficiently generate genetically modified cells and analyze the consequences of elevated and reduced expression of genes.

The research team, led by the University of Minnesotas Meri Firpo, Ph.D., included gene therapy researchers at Los Angeles Childrens Hospital, and developmental biologists at the University of Michigan.

The researchers used knockdown technology to reduce the expression of oct4, a gene known to be necessary for self renewal of mouse and human embryonic stem cells. As seen in work done with mouse cells by knockdown and other genetic means, they showed that reducing the amount of oct4 in human ES cells induced differentiation. The researchers then used a plasmid vector to transiently increase levels of oct4 in hESC. This also resulted in differentiation as expected, but with differentiation patterns similar to those seen with the knockdown. This was an unexpected result, because when expression of oct4 is up-regulated in mouse ES cells, they differentiate into a different type of cell than if the expression of oct4 is down-regulated.

"This suggests a key difference in the regulation of early development between mouse and human embryos" Firpo said. While animal models are clearly important, this research shows that scientists need human models to truly understand what happens in early human development.


'/>"/>

Contact: Dr. Meri Firpo
firpo001@umn.edu
612-625-3250
Society for Experimental Biology and Medicine
Source:Eurekalert

Page: 1

Related biology news :

1. Researchers discover way to make cells in the eye sensitive to light
2. Researchers find how protein allows insects to detect and respond to pheromones
3. Researchers Uncover Key Step In Manufacture of Memory Protein
4. NYU researchers simulate molecular biological clock
5. Researchers reveal the infectious impact of salmon farms on wild salmon
6. Researchers identify target for cancer drugs
7. Vital step in cellular migration described by UCSD medical researchers
8. ASU researchers finds novel chemistry at work to provide parrots vibrant red colors
9. UCSD researchers maintain stem cells without contaminated animal feeder layers
10. Researchers discover molecule that causes secondary stroke
11. Researchers find missing genes of ancient organism
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/3/2016)... Das DOTM (Department ... hat ein 44 Millionen $-Projekt ... einschließlich Personalisierung, Registrierung und IT-Infrastruktur, an Decatur ... Implementierung von Identitätsmanagementlösungen. Zahlreiche renommierte internationale Anbieter ... aber Decatur wurde als konformste und innovativste ...
(Date:6/2/2016)... NEW YORK , June 2, 2016   The ... (Weather), is announcing Watson Ads, an industry-first capability in which ... advertising, by being able to ask questions via voice or ... Marketers have long ... with the consumer, that can be personal, relevant and valuable; ...
(Date:6/1/2016)... , June 1, 2016 ... in Election Administration and Criminal Identification to Boost Global ... a recently released TechSci Research report, " Global Biometrics ... Region, Competition Forecast and Opportunities, 2011 - 2021", the ... billion by 2021, on account of growing security concerns ...
Breaking Biology News(10 mins):
(Date:11/30/2016)... Nov. 30, 2016 Biotest Pharmaceuticals Corporation (BPC), ... to announce the addition of its newest plasma collection ... Nebraska . The 15,200 square foot state-of-the-art facility ... 2016 and brings the total number of BPC,s plasma ... Carlisle , BPC,s Chief Executive Officer said "We are ...
(Date:11/30/2016)... DIEGO and BEIJING , ... a leading commercial provider of genomic services and solutions ... announced today that it has completed a USD $75 ... Merchants Bank Co., Ltd.,s CMB International Capital Management ( ... Innovation Investment Management Co., Ltd. ("SDIC Innovation") and Shanghai ...
(Date:11/30/2016)... ... November 30, 2016 , ... SSCI, the established leader in ... implications of the latest FDA guidance on pharmaceutical cocrystals as drug substance . ... MA. , The event follows the successful November 15th event that took ...
(Date:11/30/2016)... ... November 30, 2016 , ... With growth rates averaging more than ... years and look forward to continuing their expansion in their new office space. The ... has been traditionally favoured by the creative industries, so Random42 Scientific Communication will fit ...
Breaking Biology Technology: