Navigation Links
Researchers discover RNA repair system in bacteria
Date:10/12/2009

In new papers appearing this month in Science and the Proceedings of the National Academy of Sciences, University of Illinois biochemistry professor Raven H. Huang and his colleagues describe the first RNA repair system to be discovered in bacteria. This is only the second RNA repair system discovered to date (with two proteins from T4 phage, a virus that attacks bacteria, as the first).

The novelty of the newly discovered bacterial RNA repair system is that, before the damaged RNA is sealed, a methyl group is added to the two-prime hydroxyl group at the cleavage site of the damaged RNA, making it impossible to cleave the site again. Thus, the repaired RNA is "better than new."

This discovery has implications for protecting cells against ribotoxins, a class of toxins that kills cells by cleaving essential RNAs involved in protein translation. Because the enzyme responsible for methylation in the newly-discovered RNA repair system is the Hen1 homolog in bacteria, the finding has also implications for the understanding of RNA interference and gene expression in plants, animals, and other eukaryotes. The eukaryotic Hen1 is one of three enzymes (along with Dicer and Argonaute) essential for the generation of small noncoding RNAs of 19-30 nucleotides in RNA interference.

While the Science paper describes the mechanism of the entire RNA repair process, the article in PNAS focuses on the chemistry of the methylation reaction, specifically the crystal structure of the methyltransferase domain of bacterial Hen1. Because the eukaryotic Hen1 carries out the same chemical reaction, the study should further understanding of RNA interference in eukaryotic organisms.

"Hen1 is one of three essential enzymes in generating small noncoding RNAs for RNA interference in eukaryotes," Huang said. "We found out that Hen1 homologs exist in bacteria, but bacteria have no RNA interference. Therefore, we were very curious to find out what bacterial Hen1 is used for."

"Our studies demonstrated that bacterial Hen1 carries out the same chemical reaction as its counterpart in eukaryotes, which was not surprising," he said. "What surprised us was that, instead of involvement in RNA interference, the bacterial Hen1 is part of a RNA repair and modification system. And Hen1 is responsible for producing the repaired RNA that is 'better than new.'"


'/>"/>

Contact: William Gillespie
gillespi@illinois.edu
217-265-0722
University of Illinois at Urbana-Champaign
Source:Eurekalert

Related biology news :

1. American Association for Cancer Research to honor leading researchers at breast cancer symposium
2. Heartburn drugs deemed safe for fetuses according to Ben-Gurion University researchers
3. UMCES researchers launch freshwater marsh study
4. Researchers identify gene with possible link to infertility in mice
5. Iowa State researchers looking for catalyst that allows plants to produce hydrocarbons
6. ASCB wins NIH stimulus grant to build virtual library of cell images for researchers and public
7. Frozen assets: NIAID researchers turn to unique resource for clues to norovirus evolution
8. UT Knoxville and ORNL researchers reveal key to how bacteria clear mercury pollution
9. U of A and leading German researchers team up to build oil sands sustainability
10. Clemson researchers study energy savings with electric cars and IntelliDrive technology
11. UC nanotech researchers develop artificial pore
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/30/2017)... 30, 2017 The research team of The ... (3D) fingerprint identification by adopting ground breaking 3D fingerprint minutiae recovery ... of speed and accuracy for use in identification, crime investigation, immigration ... ... A research team ...
(Date:3/28/2017)... March 28, 2017 The report ... (Camera, Monitors, Servers, Storage Devices), Software (Video Analytics, VMS), ... - Global Forecast to 2022", published by MarketsandMarkets, the ... and is projected to reach USD 75.64 Billion by ... 2022. The base year considered for the study is ...
(Date:3/24/2017)... , Mar 24, 2017 Research and ... Access System Market Analysis & Trends - Industry Forecast to 2025" ... ... to grow at a CAGR of around 15.1% over the next ... This industry report analyzes the market estimates and forecasts for all ...
Breaking Biology News(10 mins):
(Date:4/25/2017)... April 25, 2017 Providence ... licensed its novel immune-modulating technology to an undisclosed global ... allergy. Tregitopes, pronounced T·rej·itopes, are a ... by EpiVax CEO Annie De Groot ... immunoglobulin G, an autoimmune disease therapy, Tregitopes are ...
(Date:4/25/2017)... Los Gatos, California (PRWEB) , ... April 25, ... ... of business, Analytical Services and Metrology Partners.     , Covalent’s Analytical Services ... labs. Most samples can be measured within 24 hours of receipt. There are ...
(Date:4/24/2017)... Ca (PRWEB) , ... April 24, 2017 , ... ... proteins from thermal denaturation in a cellular milieu; however, the broad application of ... lack of simple platforms with sensitive quantitative readouts. Cell-based thermal stabilization assays are ...
(Date:4/21/2017)... ... April 21, 2017 , ... The 2017 Colorado Manufacturing ... over 30 nominees and well as the first-year award for 2017 Manufacturing Women ... event was hosted by CompanyWeek and Manufacturers Edge, among other sponsors. , ...
Breaking Biology Technology: