Navigation Links
Researchers develop rapid, cost-effective early detection method for organ transplant injury
Date:8/27/2013

awbacks.

Using ddPCR for Fast, Cost-Effective Test

The researchers applied Bio-Rad's ddPCR technology to quantify graft-derived cfDNA in recent liver transplant patients and in stable patients who had undergone a transplant procedure more than six months earlier. ddPCR technology allowed them to develop a cost-effective and fast laboratory test that detects cfDNA being released into the blood stream by dying cells from the transplanted organ.

"GcfDNA from dying graft cells are the most direct and sensitive indicator of organ rejection and we needed an instrument that could measure it," said Chronix Biomedical's Chief Technology Officer and the study's senior author, Ekkehard Schuetz, MD, PhD. "ddPCR added an additional level of reliability and precision to traditional PCR."

Sequencing methods typically require batch sampling, but by using ddPCR, researchers are able to run single samples. Additionally, this method is reducing test time from three days or more to one day and costs by 90 percent. The study authors were able to address the need for donor DNA by preselecting SNPs that ensure enough heterogeneity between donor and recipient. The new blood test can also deliver results up to several days before the conventional aspartate aminotransferase (AST) and bilirubin tests for liver transplantation rejection, with the potential for an immediate positive impact on patient care.

"We will now be able to detect subclinical rejection and early intervention may allow us to avoid a full-blown rejection," said Michael Oellerich, M.D., FACB, FRCPath and Lower Saxony Distinguished Professor of Clinical Chemistry at the University Medical Center Gttingen and study Principal Investigator. "This test may be useful to personalize immunosuppression and to improve long-term outcomes."

"Detecting non-host cfDNA is the third example for the commercial potential of cfDNA diagnostics. Researchers will now be able to ex
'/>"/>

Contact: Ken Li
kli@chempetitive.com
312-532-4675
Chempetitive Group
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Study by UC Santa Barbara researchers suggests that bacteria communicate by touch
2. UC Santa Barbara researchers discover genetic link between visual pathways of hydras and humans
3. Researchers attempt to solve problems of antibiotic resistance and bee deaths in one
4. UNH researchers find African farmers need better climate change data to improve farming practices
5. Ottawa researchers to lead world-first clinical trial of stem cell therapy for septic shock
6. Researchers uncover molecular pathway through which common yeast becomes fungal pathogen
7. Researchers print live cells with a standard inkjet printer
8. Columbia Engineering and Penn researchers increase speed of single-molecule measurements
9. Researchers reveal how a single gene mutation leads to uncontrolled obesity
10. Researchers discover novel therapy for Crohns disease
11. New paper by Notre Dame researchers describes method for cleaning up nuclear waste
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/21/2015)... , April 21, 2015 ... government policies are boosting access control systems market in ... According to a recently published report by TechSci Research ... ", the access control systems market in ... billion by 2020.The access control systems market in the ...
(Date:4/14/2015)... April 14, 2015 NXT-ID, Inc. (NASDAQ: ... company focused on the growing mobile commerce market, announces ... the 2015 New York Design Awards under the category  ... York City Design Awards are part of a global ... 2,500 ratings from the marketplace, industry and judging panel. ...
(Date:4/8/2015)... , April 8, 2015  Infinisource, a ... and services, and Morpho, a leading supplier of ... to produce the market,s most advanced biometrically-enabled time ... iSolved NXG time clock is setting a bold, ... labor data for the small and mid-size employer. ...
Breaking Biology News(10 mins):Saudi Arabia Access Control Systems Market to Touch USD1.2 Billion by 2020 Says TechSci Research 2Saudi Arabia Access Control Systems Market to Touch USD1.2 Billion by 2020 Says TechSci Research 3NXT-ID's Wocket Smart Wallet Nominated for 2015 New York Design Awards in 'Product Design- Technology' 2NXT-ID's Wocket Smart Wallet Nominated for 2015 New York Design Awards in 'Product Design- Technology' 3iSolved Time Clock Offers Unmatched Accuracy due to Morpho Biometrics 2iSolved Time Clock Offers Unmatched Accuracy due to Morpho Biometrics 3
... JoVE, the Journal of Visualized Experiments will ... understand the genes that govern food-related behavior in honey ... one-step closer toward understanding and perhaps changing ... "Our technique has already helped to unravel [the] complex ...
... to clean up city parks, or scale back their driving ... for granted and ride free on the efforts of others. ... fund it. Or they run their lawn sprinklers during a ... consumption. A new report in Psychological Science in ...
... global water crisis that already has left almost 800 million ... many more in the years ahead -- are the topic ... Chemical & Engineering News . C&EN is the weekly newsmagazine ... Alex Scott, C&EN,s senior editor for Europe, points out ...
Cached Biology News:Honey bee gene targeting offers system to understand food-related behavior 2Trust in leaders, sense of belonging stir people to safeguard common goods, analysis shows 2Trust in leaders, sense of belonging stir people to safeguard common goods, analysis shows 3
(Date:4/24/2015)... DIEGO , April 24, 2015  Pfenex Inc. ... the development of biosimilar therapeutics including high value and ... its follow-on public offering of 6,000,000 shares of its ... $15.50 per share. Of the shares being offered, 2,610,000 ... shares are being offered by existing stockholders. Pfenex will ...
(Date:4/24/2015)... Seoul Semiconductor announced the expansion of ... the lighting market. In recent months, there ... in emerging markets such as Russia, South America, ... applications such as streetlight and area lighting, tunnel ... launched in 2005 the Acrich technology from Seoul ...
(Date:4/23/2015)... , April 23, 2015 /CNW/ - A new and ... award from North America,s ... Comparison won Silver in the "Sustainability, service" category ... The Brandcheck "Competitor Comparison" is a service that ... American and Global peers. This benchmarking process shows ...
(Date:4/23/2015)... WuXi PharmaTech (Cayman) Inc. (NYSE: WX ), a ... the pharmaceutical, biotechnology and medical device industries, with operations ... the United States , today announced that it ... 2015 after the New York Stock Exchange closes on ... May 14, 2015 Shanghai time). The earnings release will ...
Breaking Biology Technology:Pfenex Inc. Announces Pricing Of Follow-on Offering 2Acrich Technology from Seoul Semiconductor Adopted in Installations Worldwide 2WuXi PharmaTech Schedules First-Quarter 2015 Earnings Release 2WuXi PharmaTech Schedules First-Quarter 2015 Earnings Release 3
... Watson , SmithKline Beecham ... , Due to recent technologies in ... of potential drug candidates, it has become vital that screening assays are ... for the adaptation of a variety of assays which can be automated. ...
... M.J. Price-Jones 1 , K.T. Hughes 1 and N.R.A. Jones 2 ... UK, 2 PerkinElmer Life Sciences, P.O.Box 10, FIN-20101, Turku, Finland) , ... INTRODUCTION , ... or luminescence detection of samples in microtitration plates, tubes or on filters. , ...
... Svenja Goth , PerkinElmer Life and ... Shelton, CT 06484-4794 USA , Didier Clnet Sanofi-Aventis ... Physical Characterization Laboratory , 13, quai Jules Guesde , ... Polymorphism , Separation of transitions ...
Cached Biology Technology:Use of Novel FlashPlate Technology to Measure cAMP Accumulation in Chinese Hamster Ovary Cells Expressing Human -2 Adrenoreceptors 2Use of Novel FlashPlate Technology to Measure cAMP Accumulation in Chinese Hamster Ovary Cells Expressing Human -2 Adrenoreceptors 3Use of Novel FlashPlate Technology to Measure cAMP Accumulation in Chinese Hamster Ovary Cells Expressing Human -2 Adrenoreceptors 4Counting Cytostar-T scintillating microplates on the MicroBeta JET 2Counting Cytostar-T scintillating microplates on the MicroBeta JET 3Counting Cytostar-T scintillating microplates on the MicroBeta JET 4Counting Cytostar-T scintillating microplates on the MicroBeta JET 5Practical Applications of HyperDSC in a Pharmaceutical Laboratory 2Practical Applications of HyperDSC in a Pharmaceutical Laboratory 3Practical Applications of HyperDSC in a Pharmaceutical Laboratory 4Practical Applications of HyperDSC in a Pharmaceutical Laboratory 5
... flexible and easy to use ... control all members of the ... features a clear and easy-to-follow ... setup, user-friendly and highly sophisticated ...
Strip Removal Tool 25/Box...
The bench-top stand comfortably holds up to 6 Finnpipette Digital single- and multichannel pipettes. The crossbars are made of a grippy material to keep the pipettes firmly in place. Individual shelf...
... Analysis Note: Protein determined by Biuret Physical ... mM Tris-HCl, pH 7.6, 1 mM MgCl ... Preparation Unit Definition: One unit will ... minute at pH 9.8 at 37 C. ...
Biology Products: