Navigation Links
Researchers develop new ultrasensitive assay to detect most poisonous substance known
Date:5/1/2008

DUARTE, Calif. -- Scientists at City of Hope and the California Department of Public Health have developed a new ultrasensitive assay to detect botulinum neurotoxin. The toxin is one of the most poisonous substances known that can cause life-threatening disease, and is considered a major potential bioterrorism threat agent. The research teams work is published today in the online journal PLoS ONE (http://www.plosone.org/doi/pone.0002041).

Botulinum neurotoxin is produced by the bacterium Clostridium botulinum. When ingested, the toxin disables nerve function and can result in paralysis and even death. Botulism normally results when a person eats food tainted with C. botulinum bacteria or if a wound is infected by the bacterium. Infants, whose digestive systems are not yet fully developed, also are susceptible to the disease if the bacterium gains a foothold in their intestinal tract. The Centers for Disease Control and Prevention identify botulinum neurotoxin as one of six maximum threat bioterrorism agents due to its potency, lethality and ease of production and transport. One gram of botulinum toxin could kill more than 1 million people, according to a 2001 study in the Journal of the American Medical Association.

The new test is at least ten thousand times more sensitive and produces results much faster than the current detection method for botulinum neurotoxin, said Markus Kalkum, Ph.D., assistant professor, Division of Immunology, City of Hope, and lead researcher in the study. Wide use of the new assay would improve food safety and food processing technology, speed up and improve the diagnosis and treatment of human disease, advance the development of novel therapeutics, and greatly enhance the countrys ability to detect and defend against a bioterrorism attack.

The collaborative research team developed a test that is less expensive, faster and easier to perform than current testing options. They achieved this through the use of microscopic beads and special photochemicals that glow under ultraviolet light to achieve a heightened level of sensitivity. The microscopic beads are coated with antibodies to the botulinum neurotoxin and then mixed with the liquefied sample to be tested. The antibodies latch on to any botulinum neurotoxin molecules present in the solution and are then used to convert a special chemical into a fluorescent dye that glows in the dark when illuminated with blue or ultraviolet light. The new assay works well in liquid foods such as milk and carrot juice, and in blood serum.

The testing method has possible application in diagnosing other diseases. Researchers are investigating the potential use of antibodies for different diseases to expand the scope of the test.


'/>"/>

Contact: Shawn Le
sle@coh.org
800-888-5323
Public Library of Science
Source:Eurekalert

Related biology news :

1. USC School of Dentistry researchers uncover link between osteoporosis drugs and jaw infection
2. UCLA stem cell researchers create heart and blood cells from reprogrammed skin cells
3. Researchers discover molecular basis of a form of muscular dystrophy
4. Sunflower debate ends in Mexico, researchers say
5. Carnegie Mellon researchers urge development of low carbon electricity
6. In computer models and observations, researchers see potential for significant red tide season
7. Montana State researchers study spread of lake trout in Glacier National Park
8. Medical College researchers find dinosaur clues in fat
9. Researchers discover gene for branchio-oculo-facial syndrome
10. Researchers reveal structure of protein that repairs damage to cancer cells
11. Purdue researchers propose way to incorporate deforestation into climate change treaty
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/28/2016)... FRANCISCO and BANGALORE, India , ... of EdgeVerve Systems, a product subsidiary of Infosys (NYSE: ... provider, today announced a global partnership that will ... way to use mobile banking and payment services. ... is a key innovation area for financial services, but it ...
(Date:4/19/2016)... DUBAI , UAE, April 20, 2016 ... can be implemented as a compact web-based "all-in-one" system ... in the biometric fingerprint reader or the door interface ... requirements of modern access control systems. The minimal dimensions ... the ID readers into the building installations offer considerable ...
(Date:4/13/2016)... April 13, 2016  IMPOWER physicians supporting Medicaid patients ... a new clinical standard in telehealth thanks to a ... the higi platform, IMPOWER patients can routinely track key ... body mass index, and, when they opt in, share ... visit to a local retail location at no cost. ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... 2016   EpiBiome , a precision microbiome engineering ... debt financing from Silicon Valley Bank (SVB). The financing ... advance its drug development efforts, as well as purchase ... "SVB has been an incredible strategic partner to us ... bank would provide," said Dr. Aeron Tynes Hammack ...
(Date:6/23/2016)... LOUISVILLE, Ky. , June 23, 2016 /PRNewswire/ ... from two Phase 1 clinical trials of its ... double-blind, placebo-controlled, single and multiple ascending dose studies ... and pharmacodynamics (PD) of subcutaneous injection in healthy ... APL-2 subcutaneously (SC) either as a single dose ...
(Date:6/23/2016)... (PRWEB) , ... June 23, 2016 , ... ... is exhibiting at the Pennsylvania Convention Center and will showcase its product’s latest ... ClinCapture will also be presenting a scientific poster on Disrupting Clinical Trials in ...
(Date:6/23/2016)... June 23, 2016 ReportsnReports.com ... report to its pharmaceuticals section with historic and ... and much more. Complete report on ... pages, profiling 15 companies and supported with 261 ... http://www.reportsnreports.com/reports/601420-global-cell-culture-media-industry-2016-market-research-report.html . The Global Cell ...
Breaking Biology Technology: