Navigation Links
Researchers demonstrate nanoscale X-ray imaging of bacterial cells
Date:12/7/2009

An ultra-high-resolution imaging technique using X-ray diffraction is a step closer to fulfilling its promise as a window on nanometer-scale structures in biological samples. In the Proceedings of the National Academy of Sciences, researchers report progress in applying an approach to "lensless" X-ray microscopy that they introduced one year ago. They have produced the first images, using this technique, of biological cells specifically the intriguing polyextremophile Deinococcus radiourans. Better ability to see nanoscale structures in cells could yield important insights for evolutionary biology and biotechnology. In the case of D. radiourans, for example, it could help to settle questions about whether or how the structure of this organism's DNA-bearing nucleoid region accounts for its hardiness against ionizing radiation. Having demonstrated the resolution, reliability, and reproducibility of their technique, the researchers are now working to extend it to three-dimensional imaging of biological cells.

X-ray imaging is best known for its medical applications, such as traditional radiographs and CT scans. Yet the use of X-rays goes far beyond routine imaging. In particular, the very short wavelength of X-ray radiation allows various modes of microscopy that can reach the nanometer resolution. One of the main hurdles to high-resolution X-ray microscopy is the difficulty of producing high-quality X-ray lenses. To overcome these difficulties, so-called "lensless" microscopy methods have emerged in the last decade. A technique developed by researchers now in the biomedical physics group at Technische Universitaet Muenchen (TUM) has shown great promise for ultra-high resolution imaging of materials and life science samples.

This imaging technique, called ptychography, was first introduced in the 1970s for electron diffraction. It consists in measuring full far-field diffraction patterns as a small illumination is scanned on a sample. While its use in electron microscopy is still limited, ptychography has gained tremendous popularity in the X-ray imaging community in the last few years, thanks to the development by Franz Pfeiffer, now chair of the biomedical physics group at TUM, and his team. A critical step in the development of ptychography was published by the team one year ago in Science. The super-resolution capability of the imaging method was successfully demonstrated with a gold test structure.

Now a collaboration of the Pfeiffer group, together with researchers at University of Goettingen and at the Swiss Light Source (Villigen, Switzerland), has gone a step further and produced the first images of biological cells with the same technique.

These results, published in the Proceedings of the National Academy of Sciences, show that lensless X-ray imaging, in particular ptychography, can be used to obtain accurate maps of the electron density forming a biological sample. This type of quantitative measurement is extremely difficult with most other high-resolution techniques currently available. Moreover, biological samples are very fragile and nearly transparent to X-rays, making this type of accurate measurement even more challenging.

The Pfeiffer group is now moving beyond this success and looking into ways of improving the technique further. In particular, the team is aiming at the next milestone: three-dimensional imaging of biological samples.


'/>"/>

Contact: Andreas Battenberg
battenberg@zv.tum.de
49-892-891-2890
Technische Universitaet Muenchen
Source:Eurekalert  

Related biology news :

1. Texas AgriLife researchers helping
2. Stopping MRSA before it becomes dangerous is possible, Sandia/UNM researchers find
3. VAI researchers find long awaited key to creating drought resistant crops
4. UGA researchers lead team in discovery involving devastating freshwater fish parasite, Ich
5. Nervy research: Researchers take initial look at ion channels in a model system
6. Yerkes researchers create first transgenic prairie voles
7. North Pole wolf emails locations to researchers
8. Shape shifters: Researchers create new breed of antennas
9. Clemson researchers receive EPA grant to study carbon emission storage
10. Researchers discover biological basis of bacterial immune system
11. Researchers establish common seasonal pattern among bacterial communities in Arctic rivers
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Researchers demonstrate nanoscale X-ray imaging of bacterial cells
(Date:4/5/2017)... , April 4, 2017 KEY FINDINGS ... to expand at a CAGR of 25.76% during the ... is the primary factor for the growth of the ... https://www.reportbuyer.com/product/4807905/ MARKET INSIGHTS The global stem ... technology, application, and geography. The stem cell market of ...
(Date:4/3/2017)... 3, 2017  Data captured by IsoCode, ... detected a statistically significant association between the ... treatment and objective response of cancer patients ... predict whether cancer patients will respond to ... well as to improve both pre-infusion potency testing ...
(Date:3/30/2017)... 2017 The research team of The Hong ... fingerprint identification by adopting ground breaking 3D fingerprint minutiae recovery and ... speed and accuracy for use in identification, crime investigation, immigration control, ... ... A research team led ...
Breaking Biology News(10 mins):
(Date:10/10/2017)... (PRWEB) , ... October 10, 2017 , ... ... recipients of 13 prestigious awards honoring scientists who have made ... in a scheduled symposium during Pittcon 2018, the world’s leading conference and exposition ...
(Date:10/9/2017)... N.C. (PRWEB) , ... October 09, 2017 , ... At ... announced Dr. Christopher Stubbs, a professor in Harvard University’s Departments of Physics and Astronomy, ... Stubbs was a member of the winning team for the 2015 Breakthrough Prize in ...
(Date:10/7/2017)... (PRWEB) , ... October 06, ... ... years’ experience providing advanced instruments and applications consulting for microscopy and surface ... expertise in application consulting, Nanoscience Analytical offers a broad range of contract ...
(Date:10/6/2017)... ... October 06, 2017 , ... The HealthTech Venture Network ... at their fourth annual Conference where founders, investors, innovative practitioners and collaborators are ... pitch competition showcasing early stage digital health and med tech companies. , This ...
Breaking Biology Technology: