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Researchers could use plant's light switch to control cells
Date:10/31/2010

ne. When the researchers flashed blue light on the cell, the plant proteins interacted, causing the red fluorescent protein to rapidly move to the cell membrane, which then glowed yellow due to the merging of the red and green fluorescing proteins. The team found that this interaction was reversible and could be triggered repeatedly with light exposure.

The switch is one among several that have been designed to give researchers better control of different functions of the cell. The next step in developing the switch will be to make the interacting proteins more effective, Tucker said. The approach is expected to be applicable not only for studies in cultured cells and yeast, but also worms, fruit flies, mice and other model organisms. Eventually this method could allow researchers to test how cells in a tissue affect neighboring cells in a tissue, to guide axon growth in neurons to repair brain tissue, or even to kill cancer cells.

Tucker's new approach will be a "major boon" to those who wish to apply light activation to their own experimental systems, said Klaus Hahn, a pharmacologist at the University of North Carolina at Chapel Hill, whose lab reported on another blue-light responsive protein to control movement of mammalian cells last year.

Hahn said the "elegant work will likely see broad use, in many fields and for applications that will surprise us," and it is already going to be applied to important areas of research, such as control of gene expression.


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Contact: Ashley Yeager
ashley.yeager@duke.edu
919-681-8057
Duke University
Source:Eurekalert

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