Researchers complete draft genome sequence f...(onal Center for Tropical Agriculture (CI...)

The full genome project gathered momentum in early 2009 when 454 Life Sciences and DOE JGI each pledged the resources to use 454's Genome Sequencer FLX platform with long-read GS FLX Titanium chemistry to rapidly generate the DNA sequence data needed for the project. "This is a perfect example of how quickly things can happen when everyone is aligned behind an important cause. Most of the data for the genome were generated within 8 weeks of getting DOE JGI and 454 Life Sciences on board," said the UA's Rounsley, who led the collaboration.

More than 61 million sequencing reads were generated and assembled into a draft genome that contains an estimated 95 percent of cassava genes. It is one of the first large genome projects to primarily use 454 Life Sciences' long-read sequencing platform, which enabled both improved quality of the draft, and its rapid generation.

"We are pleased to contribute our sequencing technology to this important global initiative," explained Michael Egholm, Chief Technology Officer and Vice President of Research and Development at 454 Life Sciences. "This project, along with other recently completed complex plant genome projects, demonstrates that 454 Sequencing systems are rapidly becoming the standard for de novo sequencing and assembly."

The availability of the genome sequence enables the newly-funded project to study how cassava varieties differ from each other. "The contributions of 454 Life Sciences and DOE JGI in making the cassava genome a reality have opened a new chapter in cassava research worldwide. We're excited about the opportunity for cassava breeders to access new tools for improving a staple African crop," said Katherine Kahn, program officer with t
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