Navigation Links
Researchers catch ion channels in their opening act
Date:7/10/2008

roposed a competing idea, the "paddle model." This idea involved a large movement of the voltage sensor across the membrane. X-ray crystal structures provide snapshots of proteins in exquisite detail, allowing researchers to look at the positions of every atom.

According to Selvin, a problem with the crystal structure is that it only offers a static snapshot of what the protein looks like and provides only limited information about how different parts of the protein move. Another concern is that the conditions used to obtain protein crystals sometimes alter the original structure of the protein.

In the new study, postdoctoral researcher David Posson worked with Selvin to put the models of voltage sensor movement to the test.

They studied the voltage sensor segment in a specific ion channel called the Shaker potassium channel. This protein was first discovered in fruit flies after researchers observed that a mutation in the channel caused the flies to vigorously shake.

To preserve channels in their original state, Posson studied ion channels inserted into the membranes of frog eggs. He tested the two models using a fluorescence technique called Lanthanide resonance energy transfer (LRET) which allowed him to measure small movements in proteins. The technique involves the use of a special pair of molecular bulbs that glow either brightly or dimly depending on how far apart they are. The measurement is sensitive to movements as small as one-billionth of a meter. Posson also needed a way to control the voltage across the membrane.

He used an approach called electrophysiology that involves inserting electrodes into the frog egg. This gave him the ability to change the voltage across the membrane and regulate channel opening.

"Our approach brings together two distinct biophysical techniques, electrophysiology and fluorescence, which have been independently useful for the study of proteins," Posson said.

To map
'/>"/>

Contact: Kaushik Ragunathan
kmadabh2@illinois.edu
217-333-5802
University of Illinois at Urbana-Champaign
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. UGA researchers discover mechanism that explains how cancer enzyme winds up on ends of chromosomes
2. Researchers hack final part of the immune system code
3. Iowa State researchers study ground cover to reduce impact of biomass harvest
4. From foe to friend: Researchers use salmonella as a way to administer vaccines in the body
5. Researchers reveal types of genes necessary for brain development
6. Researchers identify cancer preventive properties in common vitamin supplement
7. UT Southwestern researchers identify new targets for RNAs that regulate genes
8. UCLA researchers clarify function of glucose transport molecule
9. UC San Diego researchers identify potential new drug candidates to combat bird flu
10. Texas A&M researchers develop tool to study complex clusters of genes
11. Weekends slow weight loss, researchers find
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/18/2014)... and their colleagues have built the first smartphone ... performance and behavioral trends. In other words, your ... if you don,t -- and how that affects ... happiness, stress, depression and loneliness to their academic ... population for example, to monitor mental health, ...
(Date:9/18/2014)... algal forests in the eastern Mediterranean Sea pose a ... distribution continues to expand as the climate warms, a ... of researchers led by Dr Adriana Vergs of UNSW ... for Advanced Studies in Spain, is published in the ... surveyed more than 1000 kilometres of coastline in Turkey ...
(Date:9/18/2014)... ultimate form of camouflage: you don,t just blend in, ... not as uncommon as you might think. Kathryn Feller, ... that the larval life stages of many marine species ... anatomy that most creatures cannot make transparent. Feller explains ... each individual eye unit with an opaque pigment to ...
Breaking Biology News(10 mins):New Dartmouth smartphone app reveals users' mental health, performance, behavior 2New Dartmouth smartphone app reveals users' mental health, performance, behavior 3New Dartmouth smartphone app reveals users' mental health, performance, behavior 4Tropical fish a threat to Mediterranean Sea ecosystems 2Transparent larvae hide opaque eyes behind reflections 2
... can counteract the effects of tumor-promoting molecules in skin ... University of Pennsylvania School of Medicine. Using animal models, ... in epithelial cells, preventing uncontrolled cell growth caused by ... published online in the Journal of Biological Chemistry, suggests ...
... suffering chronic obstructive pulmonary disease (COPD) attempt to ... contradicts the conventional wisdom on the capabilities of ... attempted regional elastic fiber repair in severe emphysema," ... Patterson and Richard Pierce of Washington University, St. ...
... neurons may have been a key factor in why ... primate relatives. In a study comparing the genomes of ... U.S. Department of Energy's Lawrence Berkeley National Laboratory (Berkeley ... high degree of genetic differences in DNA sequences that ...
Cached Biology News:Signal protein shows promise for blocking tumor promoters in skin cells 2Lungs try to repair damaged elastic fibers 2Neuron cell stickiness may hold key to evolution of the human brain 2Neuron cell stickiness may hold key to evolution of the human brain 3Neuron cell stickiness may hold key to evolution of the human brain 4
(Date:9/18/2014)... septembre 2014 Mapi, société créée à ... a été depuis 40 ans l,un des ... traditionnelles par des évaluations recentrées sur le ... impacts économiques des traitements. Logo ... A cours d,une récente conférence téléphonique avec ...
(Date:9/18/2014)... 18, 2014 OMICS Group International ... to participate in the ‘Open Access Week’ by ... OMICS Group Pharma Journals from 20-26th ... the fastest emerging Sciences, accelerating with a tremendous ... across the globe. The industry is gearing with ...
(Date:9/18/2014)... is a group of rare diseases that afflict ... Its onset hits at the fetal stage and ... the gene encoding fibroblast growth factor receptor 3 ... skeletal dysplasia, thanatophoric dysplasia (TD), a skeletal dysplasia ... is often lethal, and achondroplasia (ACH), which causes ...
(Date:9/18/2014)... Artificial sweeteners, promoted as aids to weight loss and ... intolerance and metabolic disease; and they do it in ... of the gut microbiota the substantial population of ... of experiments in mice and humans, were published today ... Eran Elinav of the Weizmann Institute,s Immunology Department, who ...
Breaking Biology Technology:1974 - 2014 - Mapi fête ses 40 ans 2Pharma and Omics Journals to Address Global Challenges during Open Access Week 2Pharma and Omics Journals to Address Global Challenges during Open Access Week 3Researchers use iPS cells to show statin effects on diseased bone 2Researchers use iPS cells to show statin effects on diseased bone 3Gut bacteria, artificial sweeteners and glucose intolerance 2Gut bacteria, artificial sweeteners and glucose intolerance 3
... Israel and NEW YORK, October 2 ... on,Company,s Diagnostic and Therapeutic Development Programs, as Well ... (Nasdaq: ROSG ), a global leader in,microRNA-based ... R&D Day on microRNAs and their significant potential ...
... /PRNewswire-FirstCall/ - Vasogen Inc.,(NASDAQ: VSGN ; TSX:VAS), a ... of technologies targeting the chronic inflammation,underlying cardiovascular and ... close of the North American financial markets,on Tuesday, ... web cast will also,be conducted on Tuesday, October ...
... 2 Othera Pharmaceuticals, Inc., a,specialty pharmaceuticals ... oncology, and inflammatory disease, has,announced the appointment ... Chief,Scientific Officer effective immediately. In this position, ... execution of Othera,s research,and clinical development programs. ...
Cached Biology Technology:Rosetta Genomics Will Host and Webcast its R&D Day on Friday, October 26, 2007 2Rosetta Genomics Will Host and Webcast its R&D Day on Friday, October 26, 2007 3Rosetta Genomics Will Host and Webcast its R&D Day on Friday, October 26, 2007 4Vasogen Announces Third Quarter Conference Call and Webcast of Bio InvestorForum 2007 Presentation 2Vasogen Announces Third Quarter Conference Call and Webcast of Bio InvestorForum 2007 Presentation 3Vasogen Announces Third Quarter Conference Call and Webcast of Bio InvestorForum 2007 Presentation 4Revised: Othera Pharmaceuticals Appoints Dr. Frederick R. Rickles Chief Scientific Officer 2Revised: Othera Pharmaceuticals Appoints Dr. Frederick R. Rickles Chief Scientific Officer 3
Attachments: Clip bar for tubes 10 to 19 mm in diameter Overall Dimension Height IC: 6 (15.2) Overall Dimension Length IC: 18.5 (46.9) Overall Dimension Width IC: 4 (10.2)...
BD FastImmune EDTA Solution. Applications: Flow Cytometry Storage Temperature: Refrigerate(2 to 8C)...
... X-Gal ,MOLECULAR BIOLOGY GRADE ,5-Bromo-4-chloro-3-indoxyl-beta-D-galactopyranoside ... Br Cl N O 6 ... (HPLC) ,Solution: Clear (2% in DMF) ... pUC and M13 ,Elemental Analysis: Agrees ...
Antibodies were affinity purified using epitopes specific to RFC1 immobilized on solid support....
Biology Products: