Navigation Links
Researchers catch ion channels in their opening act
Date:7/10/2008

roposed a competing idea, the "paddle model." This idea involved a large movement of the voltage sensor across the membrane. X-ray crystal structures provide snapshots of proteins in exquisite detail, allowing researchers to look at the positions of every atom.

According to Selvin, a problem with the crystal structure is that it only offers a static snapshot of what the protein looks like and provides only limited information about how different parts of the protein move. Another concern is that the conditions used to obtain protein crystals sometimes alter the original structure of the protein.

In the new study, postdoctoral researcher David Posson worked with Selvin to put the models of voltage sensor movement to the test.

They studied the voltage sensor segment in a specific ion channel called the Shaker potassium channel. This protein was first discovered in fruit flies after researchers observed that a mutation in the channel caused the flies to vigorously shake.

To preserve channels in their original state, Posson studied ion channels inserted into the membranes of frog eggs. He tested the two models using a fluorescence technique called Lanthanide resonance energy transfer (LRET) which allowed him to measure small movements in proteins. The technique involves the use of a special pair of molecular bulbs that glow either brightly or dimly depending on how far apart they are. The measurement is sensitive to movements as small as one-billionth of a meter. Posson also needed a way to control the voltage across the membrane.

He used an approach called electrophysiology that involves inserting electrodes into the frog egg. This gave him the ability to change the voltage across the membrane and regulate channel opening.

"Our approach brings together two distinct biophysical techniques, electrophysiology and fluorescence, which have been independently useful for the study of proteins," Posson said.

To map
'/>"/>

Contact: Kaushik Ragunathan
kmadabh2@illinois.edu
217-333-5802
University of Illinois at Urbana-Champaign
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. UGA researchers discover mechanism that explains how cancer enzyme winds up on ends of chromosomes
2. Researchers hack final part of the immune system code
3. Iowa State researchers study ground cover to reduce impact of biomass harvest
4. From foe to friend: Researchers use salmonella as a way to administer vaccines in the body
5. Researchers reveal types of genes necessary for brain development
6. Researchers identify cancer preventive properties in common vitamin supplement
7. UT Southwestern researchers identify new targets for RNAs that regulate genes
8. UCLA researchers clarify function of glucose transport molecule
9. UC San Diego researchers identify potential new drug candidates to combat bird flu
10. Texas A&M researchers develop tool to study complex clusters of genes
11. Weekends slow weight loss, researchers find
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/30/2014)... have in common? Unlike most eukaryotic multicellular organisms ... these organisms are all polyploid, meaning they have three ... 3 and 4 sets of chromosomes, respectively, and strawberries ... fact most plant species are polyploid. Polyploidy, or genome ... only recently, with the development of molecular tools, has ...
(Date:9/30/2014)... Ecker, a Salk professor and Howard Hughes Medical ... have been named recipients in the 2014 round ... (NIH) through the BRAIN (Brain Research through Advancing ... The grant, announced September 30, provides more than ... over three years. , The BRAIN Initiative, launched ...
(Date:9/30/2014)... of Medicine reports positive results of a phase ... subset of lung cancer marked by rearrangement of the ... with advanced non-small cell lung cancer testing positive for ... with 3 complete responses and 33 partial responses. Median ... disease to resume its growth after being slowed by ...
Breaking Biology News(10 mins):Gene doubling shapes the world: Instant speciation, biodiversity, and the root of our existence 2Gene doubling shapes the world: Instant speciation, biodiversity, and the root of our existence 3Gene doubling shapes the world: Instant speciation, biodiversity, and the root of our existence 4Salk scientists receive $3 million for BRAIN Initiative grant 2NEJM: Crizotinib effective in Phase 1 trial against ROS1 lung cancer 2
... pollution is costing each person in Europe around 130 - ... Nitrogen Assessment (ENA) is launched at a conference today in ... from 21 countries and 89 organizations, estimates that the annual ... - 280 billion (70 -320 billion), more than double the ...
... COLUMBUS, Ohio Stress not only sends the human immune ... the trillions of bacteria that work and thrive inside our ... important because those bacteria play a significant role in triggering ... prepared to quickly spring into action in the face of ...
... DETROIT For nearly two decades following the 1991 Gulf War, ... conflict: an unexplainable cluster of symptoms including but not limited ... 2008 that a scientific panel from the U.S. Department of ... served in the Gulf War were suffering from combinations of ...
Cached Biology News:Excessive nitrogen harms the economy and environment -- first Europe-wide assessment published 2Stress wrecks intestinal bacteria, could keep immune system on idle 2WSU receives DOD grant to determine common genetic link among Gulf War Illness patients 2
(Date:10/1/2014)... (PRWEB) October 01, 2014 What: ... software solutions driven by Semantic Web technology, today announced ... in Washington, D.C. , Smart Data solutions provide ... users to rapidly discover, understand, analyze and manage diverse ... Cambridge Semantics, will present on building a “life sciences ...
(Date:10/1/2014)... , Oct. 1, 2014  Spherix Incorporated ... the fostering and monetization of intellectual property, announced ... against VTech and Uniden in the U.S. District ... Texas has been rescheduled for November ... October 2, 2014.  The Court Order setting the ...
(Date:10/1/2014)... CA (PRWEB) September 30, 2014 ... user-friendly, cost-effective tools to address sales, training and ... has developed innovative mobile applications to turn ... outlining the agency’s latest customized mobile app creations, ... http://www.studiopmg.com/work . , In partnership with Toyota, StudioPMG ...
(Date:10/1/2014)... The U.S. Department of Labor has awarded a ... part of the Trade Adjustment Assistance Community College and ... the Department of Labor and Department of Education. , ... Perez and Secretary of Education Arne Duncan announced the ... nearly 270 colleges across the country. , The ...
Breaking Biology Technology:Cambridge Semantics to Present ‘A Useful Approach to Big Data’ at 2014 Big Data Leaders Forum 2Markman Hearing Date for VTech and Uniden Litigation Now Set for November 10, 2014 2Digital Agency Studio PMG Launches New Case Studies on Website 2U.S. Dept. of Labor Awards SIUE Nearly $10-Million Grant 2U.S. Dept. of Labor Awards SIUE Nearly $10-Million Grant 3U.S. Dept. of Labor Awards SIUE Nearly $10-Million Grant 4
... 1, 2011 Elsevier, a world-leading provider ... today announced the availability of Elsevier Urban & ... the life and health sciences, on SciVerse ScienceDirect ... under the renowned German imprint Urban & Fischer, contain ...
... researchers at the University of Toronto have developed the ... This result enables a flexible form factor, not to ... which currently relies on rigid glass. The ... Nature Photonics . OLEDs provide high-contrast and ...
... Moore,s Law, hardly a law but undeniably a persistent trend, ... transistors that fit on a chip roughly doubles. It,s why ... players to movie cameras, from tablets to supercomputers grow ... expensive. Whether the trend can continue until it runs up ...
Cached Biology Technology:Elsevier Announces Availability of Urban & Fischer Journal Archives on SciVerse ScienceDirect 2University of Toronto researchers create world's most efficient flexible OLED on plastic 2A SHARP new microscope for the next generation of biochips 2A SHARP new microscope for the next generation of biochips 3A SHARP new microscope for the next generation of biochips 4
Goat polyclonal to JIK ( Abpromise for all tested applications). Antigen: Synthetic peptide: HKKDHVFIRDEAGHGD, corresponding to amino acids 396-411 of Human JIK. Entrez Gene ID: 51347 Swi...
Mouse monoclonal [SPM325] to Xanthine Oxidase, prediluted ( Abpromise for all tested applications). Antigen: C terminal 358 amino acid fragment of human xanthine oxidase. Entrez Gene ID: 749...
Rab1A...
Drugs and NO-drug Rat Anti-conjugated Acetyl Salicylic Acid Polyclonal Antibody...
Biology Products: