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Researcher using next-generation sequencing to rapidly identify pathogens
Date:7/28/2014

xt-generation sequencing goes farther and allows us to perform metagenomic sequencing where we don't isolate the virus. Instead, we can sequence all DNA contained in a sample, which includes the host DNA, plus it reads all of the viruses in the sample too. It's a universal method to detect viruses that we have adapted and applied to veterinary diagnostics."

Hause's most recent work has led to the discovery of an influenza virus in cattle.

"A swine sample came in that we thought was influenza, but all other tests were negative," Hause said. "We found instead that this was an entirely new type of influenza. Subsequent research has shown that it is widespread in cattle, not just pigs. Now we're studying the association of this strain of bovine influenza with respiratory disease in feedlots."

Before joining Kansas State University in May, Hause was the diagnostic lab director at Newport Labs in Worthington, Minnesota. He worked on his porcine enterovirus G paper with Richard Hesse, a diagnostic virologist at the Kansas State Veterinary Diagnostic Laboratory, who helped recruit Hause to the university to further develop his next-generation sequencing methodology, which has other benefits from identifying and characterizing pathogens.

"As a virus mutates and changes, next-generation sequencing can be used to help update vaccines so they are still effective," Hause said. "Through this technology we can build a database with a collection of viruses based on where they came from and what kind of clinical presentation was seen. Then we can mine that dataset to match the vaccines or to get additional information on the pathogen. Some diseases such as flu mutate and change rapidly, and can jump from humans to pigs and back to humans, so it's important for both animal health and human health that we monitor and understand these viruses as much as possible."

The article on porcine enterovirus G, "First Identification and Characterizati
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Contact: Benjamin Hause
bhause@vet.k-state.edu
785-532-4278
Kansas State University
Source:Eurekalert  

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