Navigation Links
Researcher using next-generation sequencing to rapidly identify pathogens
Date:7/28/2014

MANHATTAN, Kansas He calls himself the bug hunter, but the target of his work consists of viruses that can only be found and identified with special methods and instruments. Benjamin Hause, an assistant research professor at the Kansas State Veterinary Diagnostic Laboratory at Kansas State University, recently published an article about one of his discoveries, porcine enterovirus G, which is an important find in the United States.

"We had isolated a virus in cells, but didn't know what it was," Hause said. "We used next-generation sequencing to identify it, and it turned out to be porcine enterovirus G, which had been described before but had never previously been found in North America."

The virus is thought to be benign and is not known to cause disease, but it had only been reported before in Europe and Asia.

"Fortunately porcine enterovirus G doesn't do much in pigs, but it raises concerns about other viruses getting through the border," Hause said. "We're not sure if this has been here for some time undetected or is a recent introduction. Coincidentally, the virus was most similar to 2012 Chinese isolates and was detected around the same time as a couple of other viruses: porcine epidemic diarrhea virus and porcine deltacoronavirus, both of which were detected in China in the same time frame prior to the U.S."

Both porcine epidemic diarrhea virus and porcine deltacoronavirus have caused major economic losses to hog producers in North America and are impacting several other aspects of the swine industry. Hause has mapped these viruses at the Kansas State Veterinary Diagnostic Laboratory as a way to ensure the reliability of the next-generation sequencing methods he uses to identify and characterize pathogens. The technique will soon be offered at the laboratory.

"As we isolate viruses, we can completely sequence their genomes," Hause said. "We can get a good understanding of what makes those viruses tick. Next-generation sequencing goes farther and allows us to perform metagenomic sequencing where we don't isolate the virus. Instead, we can sequence all DNA contained in a sample, which includes the host DNA, plus it reads all of the viruses in the sample too. It's a universal method to detect viruses that we have adapted and applied to veterinary diagnostics."

Hause's most recent work has led to the discovery of an influenza virus in cattle.

"A swine sample came in that we thought was influenza, but all other tests were negative," Hause said. "We found instead that this was an entirely new type of influenza. Subsequent research has shown that it is widespread in cattle, not just pigs. Now we're studying the association of this strain of bovine influenza with respiratory disease in feedlots."

Before joining Kansas State University in May, Hause was the diagnostic lab director at Newport Labs in Worthington, Minnesota. He worked on his porcine enterovirus G paper with Richard Hesse, a diagnostic virologist at the Kansas State Veterinary Diagnostic Laboratory, who helped recruit Hause to the university to further develop his next-generation sequencing methodology, which has other benefits from identifying and characterizing pathogens.

"As a virus mutates and changes, next-generation sequencing can be used to help update vaccines so they are still effective," Hause said. "Through this technology we can build a database with a collection of viruses based on where they came from and what kind of clinical presentation was seen. Then we can mine that dataset to match the vaccines or to get additional information on the pathogen. Some diseases such as flu mutate and change rapidly, and can jump from humans to pigs and back to humans, so it's important for both animal health and human health that we monitor and understand these viruses as much as possible."

The article on porcine enterovirus G, "First Identification and Characterization of Porcine Enterovirus G in the United States," was published in the journal PLOS ONE, http://bit.ly/1t3IfzL.


'/>"/>

Contact: Benjamin Hause
bhause@vet.k-state.edu
785-532-4278
Kansas State University
Source:Eurekalert  

Related biology news :

1. Study by UC Santa Barbara researchers suggests that bacteria communicate by touch
2. UC Santa Barbara researchers discover genetic link between visual pathways of hydras and humans
3. Study jointly led by UCSB researcher supports theory of extraterrestrial impact
4. U of Alberta researcher steps closer to understand autoimmune diseases
5. Researchers attempt to solve problems of antibiotic resistance and bee deaths in one
6. UNH researchers find African farmers need better climate change data to improve farming practices
7. Ottawa researchers to lead world-first clinical trial of stem cell therapy for septic shock
8. Researchers uncover molecular pathway through which common yeast becomes fungal pathogen
9. Researchers print live cells with a standard inkjet printer
10. Columbia Engineering and Penn researchers increase speed of single-molecule measurements
11. Researchers reveal how a single gene mutation leads to uncontrolled obesity
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Researcher using next-generation sequencing to rapidly identify pathogens
(Date:5/16/2016)... May 16, 2016   EyeLock LLC , a ... the opening of an IoT Center of Excellence in ... expand the development of embedded iris biometric applications. ... of convenience and security with unmatched biometric accuracy, making ... aside from DNA. EyeLock,s platform uses video technology to ...
(Date:5/9/2016)... UAE, May 9, 2016 Elevay ... comes to expanding freedom for high net worth professionals ... in today,s globally connected world, there is still no ... could ever duplicate sealing your deal with a firm ... passports by taking advantage of citizenship via investment programs ...
(Date:4/28/2016)... and BANGALORE, India , April 28, ... Systems, a product subsidiary of Infosys (NYSE: INFY ... announced a global partnership that will provide end ... use mobile banking and payment services.      (Logo: ... key innovation area for financial services, but it also plays ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... June 27, 2016  Global demand for enzymes ... through 2020 to $7.2 billion.  This market includes ... cleaning products, biofuel production, animal feed, and other ... and biocatalysts). Food and beverages will remain the ... increasing consumption of products containing enzymes in developing ...
(Date:6/27/2016)... San Diego, CA (PRWEB) , ... June 27, 2016 , ... ... mClinical solutions for clinical trials, announced today the Clinical Reach Virtual Patient ... and their care circle with the physician and clinical trial team. , Using the ...
(Date:6/27/2016)... BOSTON , June 27, 2016   Ginkgo ... biology to industrial engineering, was today awarded as ... a selection of the world,s most innovative companies. ... at scale for the real world in the ... organism engineers work directly with customers including Fortune ...
(Date:6/24/2016)... ... ... Researchers at the Universita Politecnica delle Marche in Ancona combed medical journal articles ... findings are the subject of a new article on the Surviving Mesothelioma website. ... blood, lung fluid or tissue of mesothelioma patients that can help point doctors to ...
Breaking Biology Technology: