Navigation Links
Research team unravels tomato pathogen's tricks of the trade
Date:11/3/2011

For decades, scientists and farmers have attempted to understand how a bacterial pathogen continues to damage tomatoes despite numerous agricultural attempts to control its spread.

Pseudomonas syringae pv. tomato is the causative agent of bacterial speck disease of tomato (Solanum lycopersicum), a disease that occurs worldwide and causes severe reduction in fruit yield and quality, particularly during cold and wet springs.

In the spring of 2010, for example, an outbreak in Florida and California devastated the harvest in those areas.

"There is not much that can be done from a farming standpoint," said Boris Vinatzer, associate professor of plant pathology, physiology and weed science, and an affiliated faculty member with the Fralin Life Science Institute at Virginia Tech. "First, farmers try to use seed that is free of the pathogen to prevent disease outbreaks. Then, there are some disease-resistant tomato cultivars, but the pathogen has overcome this resistance by losing the gene that allowed these resistant plants to recognize it and defend themselves. For the rest, there are pesticides but the pathogen has become resistant against them."

So how exactly has the pathogen evolved to consistently evade eradication efforts? This is where science steps in, and a copy of the bacterial pathogen's game plan is crucial.

Thanks to the collaborative work of Vinatzer, Virginia Bioinformatics Institute computer scientist Joao Setubal, assistant professor of statistics Scotland Leman, and their students, the genome of several Pseudomonas syrinage pv. tomato isolates have been sequenced in order to track the bacterial pathogen's ability to overcome plant defenses and to develop methods to prevent further spread.

Their findings were recently published in the Aug. 25 issue of PLoS Pathogens, a peer reviewed open-access journal published by the Public Library of Science.

The research team has studied the pathogen for nearly five years. In 2007, with resources from the Virginia Bioinformatics Institute and the Fralin Life Science Institute, Vinatzer sequenced the genome of Pseudomonas syringae pv. tomato using a Roche GS-FLX sequencer at the Virginia Bioinformatics Institute.

In 2008, Vinatzer received a $1 million, five-year Faculty Early Career Development (CAREER) Award from the National Science Foundation to continue investigation of the pathogen. Shortly thereafter, he brought Setubal, a former Virginia Tech faculty member who recently went to work for the University of Sao Paulo in Brazil, and Leman, onto the project to handle the bioinformatics and statistical aspects of the research, respectively.

First, the scientists needed to map changes in the bacterial pathogen over large spans of time. They compared DNA sequences of bacteria isolated in multiple years, dating back to 1960 and stored since then in international culture collections. Setubal, along with Nalvo Almeida, an associate professor at the Federal University of Mato Grosso do Sul, provided automated genome annotation and helped Vinatzer with identification and analysis of mutations that distinguished the sequenced genomes.

"One of the questions we asked was, did the bacteria isolated in 2000 directly evolve from the bacteria isolated in 1975, or did they evolve independently from an ancestor that lived further back in time, maybe 100 to 200 years ago?" said Vinatzer.

To answer this question, Leman, Vinatzer, and Rongman Cai of Lixian, China, a Ph.D. student in plant pathology, physiology, and weed science at Virginia Tech, used statistical methods to correlate the differences in the DNA sequences between the isolated bacteria with the years in which they were isolated to determine if the bacteria progressively became more different from the first bacteria isolated in 1960.

The research team found that the pathogen likely evolved on a relatively recent time scale and continues to adapt to the tomato by minimizing its recognition by the tomato immune system. This suggests that new pathogen variants with increased virulence are spreading around the globe unobserved, presenting a potential threat to biosecurity. Ultimately, it calls for more precise methods of pathogen identification to replace outdated taxonomic descriptions that were established at a time when it was impossible to classify bacteria precisely because the necessary molecular techniques had not been developed yet.

This particular pathogen is important to study because "it has been used as a model pathogen by many labs around the world and is economically significant because it reduced tomato quality and yield," said Vinatzer. He is extending the research project to investigate where the pathogen originally evolved, how it is spreading around the globe, and what can be done to interfere with its spread, including how the tomato might be engineered to be more resistant to the pathogen.


'/>"/>

Contact: Lindsay Key
ltkey@vt.edu
540-231-6594
Virginia Tech
Source:Eurekalert  

Related biology news :

1. Wistar Institute researcher receives New Innovator award from NIH
2. NC State researchers get to root of parasite genome
3. White Mountain Research Station to host climate change conference
4. Stevens awarded $1M for advanced biofuels research
5. Researchers find animal with ability to survive climate change
6. Researchers find an essential gene for forming ears of corn
7. Researchers note differences between people and animals on calorie restriction
8. Researcher working on destruction of chemical weapons
9. Researchers study acoustic communication in deep-sea fish
10. Researchers discover that growing up too fast may mean dying young in honey bees
11. The Rett Syndrome Research Trust launches operations
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Research team unravels tomato pathogen's tricks of the trade
(Date:2/1/2016)... , Feb. 1, 2016  Today, the first day ... announced plans to develop a first of its kind ... of IBM Watson. In the first application of ... (NYSE: IBM ), and Welltok will create a ... assessments with cognitive analytics, delivered on Welltok,s health optimization ...
(Date:1/25/2016)... Jan. 25, 2016  Glencoe Software, the world-leading supplier ... publication industries, will provide the data management solution OMERO ... Photo ... Phenotypic analysis measures the characteristics ... allowing comparisons between states such as health and disease, ...
(Date:1/20/2016)... Jan. 20, 2016   MedNet Solutions , an ... spectrum of clinical research, is pleased to announce the ... achievements are the result of the company,s laser focus ... eClinical , it,s comprehensive, easy-to-use and highly affordable ... --> Key MedNet growth achievements in 2015 include: ...
Breaking Biology News(10 mins):
(Date:2/10/2016)... 2016 Early-career researchers from ... , Uganda and Yemen ... and nutrition   Indonesia , Nepal ... and Yemen are being honored for their accomplishments ... also celebrated for mentoring young women scientists who are pursuing careers in ...
(Date:2/10/2016)...  IsoRay, Inc. (NYSE MKT: ISR), a medical technology ... applications for the treatment of prostate, brain, lung, head ... results for the second quarter and six months of ... --> --> Revenue was ... which ended December 31, 2015, a 12% increase compared ...
(Date:2/10/2016)... , ... February 10, 2016 , ... LATHAM, NEW YORK... ... the SPIE Photonics West conference in San Francisco’s Moscone Center from February ... the same venue. , These latest InGaAs PIN diode standard packages feature a ...
(Date:2/10/2016)... ... 10, 2016 , ... Cenna Bioscience Inc., an emerging biopharmaceutical company focused on ... today it has been selected to present at the Cavendish Global Health Impact Forum ... The purpose of the Forum is to help family offices and foundations develop ...
Breaking Biology Technology: