Research sheds light on the mechanics of gene tra...( The molecular machinery behind gene ...)

The molecular machinery behind gene transcription -- the intricate transfer of information from a segment of DNA to a corresponding strand of messenger RNA -- isn't stationed in special "transcription factories" within a cell nucleus, according to Cornell researchers. Instead, the enzyme RNA polymerase II (Pol II) and other key molecules can assemble at the site of an activated gene, regardless of the gene's position.

The findings, published in the Dec. 28, 2007, issue of the journal Molecular Cell, are the result of an ongoing collaboration between the laboratories of John T. Lis, the Barbara McClintock Professor of Molecular Biology and Genetics, and Watt W. Webb, professor of applied physics and the S.B. Eckert Professor in Engineering. Jie Yao, the paper's lead author, recently finished his Ph.D. at Cornell under Webb.

Using multiphoton microscopy, a technique developed by Webb that allows high-precision 3D imaging in living cells, the researchers observed polytene chromosomes -- giant, multistranded chromosomes in the salivary gland tissue of fruit flies that have hundreds of sets of the genome instead of the usual two sets in conventional cells.

They activated heat shock genes, which protect cells from sudden rises in temperature, and watched them in real time as they began to be transcribed. The researchers also tagged Pol II with a fluorescent marker to track its movements within the nucleus.

While some reports have suggested that activated genes move to a specific nuclear location for transcription, the Cornell research supports the traditional view that gene activation is not dependent on movement to special locations, or so-called "transcription factories," said Lis.

"You see the genes decondense and fill up with polymerase, but they're not moving anywhere -- they don't collect in a single place," he said. Instead, the transcription machinery assembles at the called-upon locus, regardless of its position in the nucleus.

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