Navigation Links
Research into synthetic antibodies offers hope for new diagnostics
Date:1/24/2011

which respond to a given injected protein by producing a protein-specific antibody, which may then be extracted.

In earlier work, Johnston's group showed that high-affinity antibody mimics can be produced synthetically by simple means. Their technique turns the traditional production approach on its head. Rather than beginning with a given protein and trying to generate a corresponding antibody, the new method involves building a synthetic antibody first, later determining the protein it effectively binds with, by screening it against a library of potential protein mates.

The first step in this process is to generate random strings of 20 amino acids. Roughly 10,000 such random peptides are then spotted onto a glass microarray slide. The protein one is seeking an antibody to is screened against this random sequence array and peptides with high binding affinity are identified. Two such peptides can be linked together to form a synbody, whose binding affinity is the product of each separate peptide. In this way, two weakly binding peptides join forces to form a high affinity unit, useful for investigations into the proteome, the vast domain of proteins essential to virtually all biological processes.

In the PloS ONE study, lead author Matthew Greving and his collegues describe a strategy for further refinement of binding affinity in random sequence peptides. "The problem," Johnston explains, is that the microarray contains about 10,000 peptides, but that is less than a quadrillionth of the possible peptides by sequence. So we're sampling a very small part of the sequence space. " A consequence of this is that the probability of generating a 20 amino acid sequence, that binds with optimal affinity, is pretty low.

To improve sequence affinity, a lead sequence is first selected. In the study, one such sequence was the 17 amino acid peptide TNF-1, a key regulator of immune cells. The lead sequence is then used as a template f
'/>"/>

Contact: Joe Caspermeyer
Joseph.Caspermeyer@asu.edu
Arizona State University
Source:Eurekalert  

Page: 1 2 3 4

Related biology news :

1. Wistar Institute researcher receives New Innovator award from NIH
2. NC State researchers get to root of parasite genome
3. White Mountain Research Station to host climate change conference
4. Stevens awarded $1M for advanced biofuels research
5. Researchers find animal with ability to survive climate change
6. Researchers find an essential gene for forming ears of corn
7. Researchers note differences between people and animals on calorie restriction
8. Researcher working on destruction of chemical weapons
9. Researchers study acoustic communication in deep-sea fish
10. Researchers discover that growing up too fast may mean dying young in honey bees
11. The Rett Syndrome Research Trust launches operations
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Research into synthetic antibodies offers hope for new diagnostics
(Date:7/11/2014)... from the Max Planck Institute for Medical Research in ... understanding photosynthesis, the process by which the Earth first ... and which is therefore crucial for all higher forms ... the first direct visualization of a crucial event in ... specific protein complex, photosystem II, splits water into hydrogen ...
(Date:7/11/2014)... of kidney injury related to the use of hydroxyethyl ... HES molecules, according to a report in Anesthesia ... Research Society (IARS). , The "total mass of HES ... human renal proximal tubule cells (PTCs), concludes the laboratory ... Hospital Wrzburg, Austria. Other factorssuch as differences in ...
(Date:7/11/2014)... is evidence that under the normal circumstances, ... development, maintain neuronal environment, and exhibit therapeutic ... neurodegenerative disease. Previous studies have found that ... after chemical induction have reduced viability, which ... Prof. Xiaodong Yuan, Kailuan General Hospital, Hebei ...
Breaking Biology News(10 mins):A first direct glimpse of photosynthesis in action 2In lab studies, hydroxyethyl starch has direct harmful effects on kidney cells 2
... from the University of Alberta, the Yerkes National Primate ... Center has successfully transplanted insulin-producing neonatal porcine islet cells ... promising intermediate solution to the critical supply problem in ... U of A and Emory, along with recent work ...
... Researchers at the University of Pennsylvania School of Medicine ... the host cell's ability to survive by binding to ... enzyme from activating the cell's immune system. These findings ... imply new approaches to treating AIDS, inflammatory diseases such ...
... cartilage (cartilage covering the ends of bones where they ... or illness, and can lead to degenerative disease. Treatments ... achieved limited results, but currently there is no method ... engineering involving the delivery of therapeutic proteins to the ...
Cached Biology News:Diabetes researchers pioneer islet cell xenotransplantation in primate studies 2HIV accessory protein disables host immunity via receptor-protein intermediary 2Stem cells from muscles can repair cartilage 2Stem cells from muscles can repair cartilage 3
(Date:7/11/2014)... (PRWEB) July 11, 2014 Cayenne ... company specializing in the soft tissue reconstruction segment, ... Anchor System for surgeries involving the shoulder and ... through a novel, inserter-controlled deployment method. The unique ... partial deployment, anchor pull-out, or anchor displacement (also ...
(Date:7/10/2014)... Mass. (PRWEB) July 10, 2014 Terascala, ... today announced that Alan Swahn, a former vice president ... executive team as vice president of marketing. Terascala’s software ... Dell and NetApp create the highest performance and most ... been appointed to guide Terascala’s channel expansion and broaden ...
(Date:7/10/2014)... 2014 Senior supply chain management executives ... addressed the challenges of “Reducing Cost, Lead Time, & ... strategic sourcing. Describing the partnership of the Bio Supply ... Management Institute ( SCMI) of the University of ... at the Kroc Institute of Peace and Justice in ...
(Date:7/10/2014)... July 10, 2014 Research and Markets ... the "International Photonic Integrated Circuit (Monolithic Integration, ... 2019" report to their offering. ... concept of photonic integration traces its roots in ... promise of photonic integration went unexplored and unfulfilled ...
Breaking Biology Technology:Cayenne Medical Launches SureLock™ All-Suture Anchor System for Shoulder and Extremity Surgery 2Cayenne Medical Launches SureLock™ All-Suture Anchor System for Shoulder and Extremity Surgery 3Terascala Expands Executive Team 2Terascala Expands Executive Team 3Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 2Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 3Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 4International Photonic Integrated Circuit (Monolithic Integration, Hybrid Integration, Module Integration) Market - Forecasts to 2019 2
... (Pink Sheets: BSTC.PK) today announced that it sold,200,000 ... private placement,offering to an investment fund, at a ... to the Company of $2,100,000., About BioSpecifics ... biopharmaceutical company that has,developed and licensed injectable collagenase ...
... is seeking nominations for the Third Annual Greater ... March 18, 2008. Those,interested in nominating a company ... The four award categories are: Entrepreneurial, Best ... Award. Nominators will,be asked to submit documentation and ...
... multiplexing module, SANTA ROSA, Calif., Jan. 14 ... provider of optical components for industrial,applications, is to ... technology. The compact LED combining module, which utilizes,proprietary ... medical instrumentation. The ZoroLight LED module will be,on ...
Cached Biology Technology:Bookham to Enter Biomedical Illumination Market 2Bookham to Enter Biomedical Illumination Market 3
Caliper offers a wide array of standard LabChip products designed to be used in combination with our AMS 90 and LabChip 3000 microfluidic platforms....
... provide fast and reliable automation for ... drug development laboratories. Staccato Mini-Workstations offer ... automate basic liquid handling and material ... with either Caliper's time proven CLARA™ ...
Caliper offers a wide array of standard LabChip products designed to be used in combination with our AMS 90 and LabChip 3000 microfluidic platforms....
... Fermentation System to achieve high ... and maximum information output 4 ... precision with small-scale requirements provides users ... with E.coli, Pichia pastoris, yeast, fungi ...
Biology Products: