Navigation Links
Reprogram stem cells with heart tissue, which might improve treatments for heart disease
Date:5/19/2010

This release is available in Spanish.

Spanish researchers have employed for the first time adult cells extracted from a human heart to turn stem cells from adipose tissue into cardiac myocytes. In other words, they achieved to "reprogram" adult stem cells, which might improve treatments for heart disease therapeutical.

At present, the use of stem cells in treatments for heart disease is one of the most common practices. However, working with stem cells without targeting heart tissue negatively affects the efficacy of treatments. Therefore, inducing cell differentiation into cardiac muscle (cardiomyocytes) may be one of the best options in the treatment of these pathologies.

For the purpose of this study, researchers isolated adult human stem cells from lipoaspiration. Subsequently, these cells were temporarily permeabilized and exposed to a human-auricle cell extract. Then, these cells were recovered in culture.

Morphological Changes

After 21 days in culture, the cells differentiated towards a cardiac myocyte phenotype, which was demonstrated by expression of morphological changes (appearance of binuclear cells with striated fibers and ramifications), detection of cardiospecific markers through inmunofluorescence, and the presence of cardiac muscle-related genes that were analysed through RT-PCR; and finally, by expression of reverse transcription. Thus, mesenchimal stem cells acquired a cardiac phenotype.

This study was conducted by Macarena Pern, Juan A. Marchal, Elena Lpez, Manuel Jimnez-Navarro, Houria Boulaiz, Fernando Rodrguez-Serrano, Esmeralda Carrillo, Gema Snchez-Espn, Eduardo de Teresa, David Tosh y Antonia Arnega, researchers from the University of Jaen (Spain), the University of Granada, the Hospital Clnico Universitario of Malaga and the University of Bath (United Kingdom). It is going to be published on the Journal Cytotherapy, the official reporting organ of the International Society for Cellular Therapy (ISCT).

This technique could be used in the future for regeneration of cardiac muscles through the use of cells directly extracted from the patient. However, physicians have remarked that, at present, this research is in its earlier stages, and it will be a long time until it has any therapeutical use.

Currently, researchers are preparing a new approach for introducing the cell extract into the target cell (by using a cell microinjector) that will allow them to obtain a larger number of viable differentiated cells, which is essential for their having any therapeutical use. The following step is to use animal models to validate differentiated cells' functionality. Finally, a number of clinical trials should be conducted to assess the viability of this technique in human patients.


'/>"/>

Contact: Antonia Aranega Jimenez
aranega@ugr.es
34-958-243-534
University of Granada
Source:Eurekalert

Related biology news :

1. March of Dimes awards $250,000 prize to scientist who discovered how to reprogram human cells
2. Fate Therapeutics announces creation of small molecule platform for commercial-scale reprogramming
3. Tumor suppressor pulls double shift as reprogramming watchdog
4. Reprogramming human cells without inserting genes
5. Study reveals a reprogrammed role for the androgen receptor
6. Study finds blood cells can be reprogrammed to act as embryonic stem cells
7. Researchers piggyback to safer reprogrammed stem cells
8. Reprogrammable cell type depends on a single gene to keep its identity
9. Salk researchers successfully reprogram keratinocytes attached to a single hair
10. Yamanaka eliminates viral vector in stem cell reprogramming
11. Salk researchers reprogram adult stem cells in their natural environment
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:11/19/2016)... 2016 Securus Technologies, a leading provider of ... investigation, corrections and monitoring, announced today that it has ... have an independent technology judge determine who has the ... tech/sophisticated telephone calling platform, and the best customer service. ... most of what we do – which clearly is ...
(Date:11/15/2016)... , Nov. 15, 2016  Synthetic Biologics, Inc. ... therapeutics focused on the gut microbiome, today announced ... 25,000,000 shares of its common stock and warrants ... at a price to the public of $1.00 ... Synthetic Biologics from the offering, excluding the proceeds, ...
(Date:6/27/2016)... Research and Markets has announced the addition ... to their offering. The report ... to grow at a CAGR of 12.28% during the period 2016-2020. ... in-depth market analysis with inputs from industry experts. The report covers ... The report also includes a discussion of the key vendors operating ...
Breaking Biology News(10 mins):
(Date:11/30/2016)... BILLERICA, Massachusetts , 30. November 2016 ... und Technologieunternehmen, hat heute die Unterzeichnung einer ... gegeben. Diesen zufolge wird Evotec AG Screeningleistungen ... und shRNA-Bibliotheken bereitstellen. Der Zugriff auf diese ... Bereich Screening eröffnet einen schnelleren Weg zur ...
(Date:11/30/2016)... ... November 30, 2016 , ... SSCI, the established leader in small-molecule cocrystal ... the latest FDA guidance on pharmaceutical cocrystals as drug substance . The ... , The event follows the successful November 15th event that took place in ...
(Date:11/30/2016)... ... November 30, 2016 , ... On 28 November 2016, the International Union ... nihonium (Nh), moscovium (Mc), tennessine (Ts), and oganesson (Og), respectively for element 113, ... earlier proposed by the discoverers have been approved by the IUPAC Bureau. The IUPAC ...
(Date:11/30/2016)... ... November 30, 2016 , ... ... groundbreaking venture founded by Arianna Huffington, as part of the Thrive Global pop-up ... Explorer Kit, enabling purchasers to explore the microorganisms in their gut, collectively known ...
Breaking Biology Technology: