Navigation Links
Reproductive biologists move in vitro fertilization knowledge forward
Date:10/28/2013

, the biochemist adds.

Any better understanding of the cascade of events, where mistakes in IVF often occur, would be useful, Visconti says. Conducting experiments with mouse sperm in vitro, he and colleagues at UMass Amherst, with others at Weill Cornell Medical College, University of Hawaii Medical School, Asahikawa Medical University Japan, Universidad Nacional de Rosario and Facultad de Medicina Argentina and Universidad Nacional Autnoma de Mxico made two discoveries that should help.

In the PNAS study, the researchers experimented with increasing intracellular calcium using the calcium ionophore, A23187. Calcium is known to play a role late in the sperm capacitation process, after many earlier steps in pathways that depend on soluble adenylyl cyclase, cyclic adenosine monophosphate (cAMP), protein kinase A (PKA) and other enzyme cascades have taken place.

A major drawback of adding calcium ionophore, however, is that it overwhelms the sperm with too much and quickly immobilizes them, Visconti notes. But he and colleagues found that they could get around this by simply washing the excess away. Thus they basically bypass early capacitation stages by applying the calcium ionophore, wash it away, and the sperm proceed with capacitation.

Under these conditions, the authors report, ionophore-treated sperm fertilized 80 percent of eggs, which developed into normal offspring. Their data indicate that ionophore-treated mouse sperm can fertilize ova even when the cAMP/PKA signaling pathway is inhibited.

Visconti credits his co-authors Hiroyuki Tateno in Japan and Ryuzo Yanagimachi in Hawaii with the idea of washing ionophore away after use. He points out, "Until they conceived it, no one had thought of this trick. They did the first experiments," Visconti explains. "Later, our experiments demonstrated that by treating the sperm with calcium ionophore, we were activating these cells far downstream of the normal biologi
'/>"/>

Contact: Janet Lathrop
jlathrop@admin.umass.edu
413-545-0444
University of Massachusetts at Amherst
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Sex over survival: Reproductive trait in fish impedes tissue regeneration
2. Reproductive BioMedicine Online publishes study on assisted reproduction
3. Reproductive tract secretions elicit ovulation
4. Stanford scientist uncovers the reproductive workings of a harvester ant dynasty
5. Bisphenol A affects sex-specific reproductive behaviors in a monogamous animal species
6. Sex of early birds suggests dinosaur reproductive style
7. Report from the front lines of personalized reproductive medicine revolution
8. Rare cliffhanging plant species uses unique reproductive strategy
9. BPA could affect reproductive capabilities, cause infection of the uterus
10. Highly exposed to phthalates as fetuses, female mice have altered reproductive lives
11. Reproductive health providers should discuss environmental exposure risks with patients
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Reproductive biologists move in vitro fertilization knowledge forward
(Date:6/30/2015)... To bolster its efforts and commitment to fighting ... the addition of two new team members. ... David Raviv will act as head of strategic ... to providing the most secure solutions for the enterprise market. ... 7 Technologies, a provider of security and management products, which ...
(Date:6/29/2015)... June 24, 2015 Research ... the "Latin America Biomedical Sensors Market - Growth, ... The Latin America Biomedical Sensors market is ... of 2.04% over the period 2014-2020 The ... to the genetic formulation of each individual. These sensors ...
(Date:6/25/2015)... 2015 According to a ... & Area), Technology, Material (Optical Prism, Piezoelectric, Capacitive ... & Others) & Geography - Global Forecast to ... market is expected to reach $14,500.07 Million by ... Browse 76 market Tables and 109 Figures ...
Breaking Biology News(10 mins):HYPR Corp. Expands Biometric Authentication Team with Enterprise All Stars 2HYPR Corp. Expands Biometric Authentication Team with Enterprise All Stars 3Latin America Biomedical Sensors Market Report 2015-2020 2Fingerprint Sensors Market Worth $14,500.07 Million by 2020 2Fingerprint Sensors Market Worth $14,500.07 Million by 2020 3Fingerprint Sensors Market Worth $14,500.07 Million by 2020 4
... available in German . , Helmholtz Zentrum ... European Union, has been expanded to include six partners from ... partners will join the effort of the other 22 members ... improve open access to high-quality mouse models. Animal models have ...
... Energy (DOE) Joint Genome Institute (JGI) now has a ... 13th Annual Applied Ergonomics Conference held March 22-25, 2010 ... highlights ergonomic innovations from institutions and multinational corporations. The ... for the Ergonomic Program Improvement Initiatives category. Ergonomics ...
... preliminary study by New Zealand company Plant & Food ... breathing in some types of asthma. Researchers found ... lung inflammation with a multi-action assault in allergy-induced asthma. ... the natural defence mechanisms in lung tissue by both ...
Cached Biology News:Biomedical research infrastructure goes global: EU consortium Infrafrontier has new partners 2Breathe easy: A natural fruit compound may help asthma 2
(Date:7/28/2015)...  Kerastem Technologies announce that the Company has received ... For Biologics Evaluation and Research (CBER) Office of Cellular, ... investigating the safety and feasibility of the Company,s technology ... baldness (androgenic alopecia). The phase II study, ... in Europe and ...
(Date:7/28/2015)... , July 28, 2015 CSL ... Drug Administration has accepted for review the company,s Biologics ... VIII single-chain (rVIII-SingleChain) for the treatment of hemophilia A. ... endpoints. Hemophilia A is ... factor VIII; nearly all affected patients are male. People ...
(Date:7/28/2015)... Israel , July 28, 2015 ... medicine company utilizing its proprietary plant-based rhCollagen technology ... Shurtz has been appointed Senior Director of Business ... (Photo: http://photos.prnewswire.com/prnh/20150728/246955 ) , ... in marketing, regulatory and business strategy. Prior to ...
(Date:7/27/2015)... CHICAGO and FRANKLIN, Tenn. ... Inc. ("Capella"), a privately-owned  premier provider of health care ... companies in the U.S., today announced that its ... has signed a definitive agreement with Medical Properties ... the acquisition of Capella for $900 million in cash.  The ...
Breaking Biology Technology:Kerastem Receives Conditional Approval from FDA for Alopecia Trial 2U.S. FDA Accepts for Review CSL Behring's Biologics License Application for Its Novel rVIII-SingleChain Therapy for Patients with Hemophilia A 2U.S. FDA Accepts for Review CSL Behring's Biologics License Application for Its Novel rVIII-SingleChain Therapy for Patients with Hemophilia A 3CollPlant Names Shomrat Shurtz Senior Director of Business Development 2Capella Healthcare Announces Agreement With Medical Properties Trust, Inc. 2Capella Healthcare Announces Agreement With Medical Properties Trust, Inc. 3Capella Healthcare Announces Agreement With Medical Properties Trust, Inc. 4Capella Healthcare Announces Agreement With Medical Properties Trust, Inc. 5
... DALLAS, Texas , June 15, 2010 ... Brazil , Russia , India , China (BRIC)" published by ... million in 2009 and is expected to reach $570.56,million in ... , , ...
... , ... filed alleging that the DePuy ASR hip replacement was defectively designed and that DePuy did ... , ... A lawsuit has been filed against hip replacement manufacturer DePuy Orthopaedics, Inc over failure of ...
... type of baker,s yeast ( Saccharomyces cerevisiae ) has ... found in agricultural waste and hardwoods. Researchers writing in ... describe the creation of the new S. ... growth rate and final biomass concentration on sugar media ...
Cached Biology Technology:Diabetes Care Devices Market In Brazil, Russia, India, China (BRIC) Worth $570.56 Million In 2014 2Diabetes Care Devices Market In Brazil, Russia, India, China (BRIC) Worth $570.56 Million In 2014 3Diabetes Care Devices Market In Brazil, Russia, India, China (BRIC) Worth $570.56 Million In 2014 4First DePuy ASR Hip Replacement Lawsuit Filed in the US 2
... antibody raised against a partial recombinant IFNA2. ... partial recombinant protein is produced in E. coli. ... IU/ mg. OMIM: 147562, ... Lot Number: MM951100564 MA Code: ...
... V, is used to coat the inner wall ... the source of the DNA that enters target ... gun. The station includes a tubing support cylinder, ... Nalgene nitrogen regulator hose, two 0.1875 inch barb-to-male ...
... Microarray Hybridization Wash Pack provides ... microarray slides. Preparation Quality Clarifier: Ready-to-use ... use with SigmaScreen(tm) APS Coated Slides ... Microarray Hybridization Blocking Buffer: 1L ...
Mouse monoclonal antibody raised against a partial recombinant NR4A2. NCBI Entrez Gene ID = NR4A2...
Biology Products: