Requirements for MS-based proteomic biomarker development 51
Protein tomography 51
Protein biochips/microarrays and biomarkers 51
Antibody-based biomarker discovery 52
Detection of biomarkers using peptide array technology 52
Protein nanobiochip 52
Quantification of protein biomarkers 53
Mass spectrometry for quantification of protein biomarkers 53
Real-time PCR for quantification of protein biomarkers 53
CyTOF for quantification of biomarkers 53
Search for biomarkers in body fluids 54
Challenges and strategies for discovey of protein biomarkers in plasma 54
3-D structure of CD38 as a biomarker 55
BD™ Free Flow Electrophoresis System 55
Isotope tags for relative and absolute quantification 56
Plasma protein microparticles as biomarkers 56
Proteome partitioning 57
Stable isotope tagging methods 57
Technology to measure both the identity and size of the biomarker 58
Biomarkers in the urinary proteome 58
Peptides in body fluids and tissues as biomarkers of disease 58
Analysis of peptides in bodily fluids 59
Serum peptidome patterns 60
SISCAPA method for quantitating proteins and peptides in plasma 60
Comparison of proteomic profiling technologies for discovery of biomarkers 60
Verification for interlaboratory reproducibility of protein biomarkers 61
Significance of similar protein biomarkers in different tissues 61
Glycomic technologies 62
Metabolomic technologies 62
Genome-wide association studies for identification of metabolic biomarkers 63
Lipid profiling 63
Mass spectrometry-based kits for discovery of metabolic biomarkers in plasma 64
Role of metabolomics in biomarker identification and pattern recognition 64
Urinary profiling by capillary electrophoresis 64
Validation of biomarkers in large-scale human metabolomics
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