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Requirements for MS-based proteomic biomarker development 51

Protein tomography 51

Protein biochips/microarrays and biomarkers 51

Antibody-based biomarker discovery 52

Detection of biomarkers using peptide array technology 52

Protein nanobiochip 52

Quantification of protein biomarkers 53

Mass spectrometry for quantification of protein biomarkers 53

Real-time PCR for quantification of protein biomarkers 53

CyTOF for quantification of biomarkers 53

Search for biomarkers in body fluids 54

Challenges and strategies for discovey of protein biomarkers in plasma 54

3-D structure of CD38 as a biomarker 55

BD™ Free Flow Electrophoresis System 55

Isotope tags for relative and absolute quantification 56

Plasma protein microparticles as biomarkers 56

Proteome partitioning 57

Stable isotope tagging methods 57

Technology to measure both the identity and size of the biomarker 58

Biomarkers in the urinary proteome 58

Peptides in body fluids and tissues as biomarkers of disease 58

Analysis of peptides in bodily fluids 59

Serum peptidome patterns 60

SISCAPA method for quantitating proteins and peptides in plasma 60

Comparison of proteomic profiling technologies for discovery of biomarkers 60

Verification for interlaboratory reproducibility of protein biomarkers 61

Significance of similar protein biomarkers in different tissues 61

Glycomic technologies 62

Metabolomic technologies 62

Genome-wide association studies for identification of metabolic biomarkers 63

Lipid profiling 63

Mass spectrometry-based kits for discovery of metabolic biomarkers in plasma 64

Role of metabolomics in biomarker identification and pattern recognition 64

Urinary profiling by capillary electrophoresis 64

Validation of biomarkers in large-scale human metabolomics

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