Navigation Links
Regenerated cells may restore vision after corneal dysfunction
Date:6/13/2012

Philadelphia, PA, June 14, 2012 Regenerative medicine, or the use of specially grown tissues and cells to treat injuries and diseases, has been successful in treating disorders of a number of organs, including heart, pancreas, and cartilage. However, efforts to treat disorders of the corneal endothelium, a single cell layer on the inner surface of the cornea, with regenerative techniques have been less effective. Now, a group of scientists has developed a method that enhances the adhesion of injected corneal endothelial cells (CECs), allowing for successful corneal transplantation to repair pathological dysfunctions. Their results are published online today in advance, in the July issue of The American Journal of Pathology.

"Corneal endothelial dysfunction is a major cause of severe visual impairment, since the cells maintain the transparency of the cornea," explains lead investigator Noriko Koizumi, MD, PhD, of the Department of Biomedical Engineering, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Japan. "Injected cultured CECs can be washed off by aqueous humor flow, resulting in poor adhesion of the cells injected onto the corneal tissue. Previous studies demonstrated that Rho-associated kinase (ROCK) signaling interferes with adhesion. We found that transplanting cultivated CECs in combination with a low-molecular weight compound that inhibits ROCK (ROCK inhibitor Y-27632), successfully achieved the recovery of corneal transparency."

Using rabbit cells, researchers cultivated CECs in the lab and injected them into the anterior chamber of rabbit eyes with damaged corneal endothelia. Based on the recovery of the corneal endothelial function, they found that when the cultivated cells were injected along with Y-27632, the rabbit corneas regained complete transparency 48 hours after injection. In contrast, rabbit CECs injected without Y-27632 resulted in hazy and severely swollen corneas. No complications related to cell injection therapy were observed and reconstructed corneal endothelium with Y-27632 exhibited normal hexagonal cell shape.

Since rabbit CECs are highly prolific in vivo, the scientists performed another round of experiments with monkey CECs, which are more similar to those in humans. The transplantation of CECs in these primates also achieved the recovery of long-term corneal transparency with a monolayer of hexagonal cells, suggesting that cell adhesion modified by ROCK inhibitor may be an effective treatment for human corneal endothelial disorders.

Although surgical techniques to replace the injured corneal endothelium have been developed, these procedures are technically difficult and challenging due to a shortage of donor corneas. "The novel strategy of using a cell-based therapy combined with a ROCK inhibitor may ultimately provide clinicians with a new therapeutic modality in regenerative medicine, not only for treatment of corneal endothelial dysfunctions, but also for a variety of pathological diseases," Dr. Koizumi concludes.


'/>"/>

Contact: David Sampson
ajpmedia@elsevier.com
215-239-3171
Elsevier Health Sciences
Source:Eurekalert  

Related biology news :

1. Magical state of embryonic stem cells may help overcome hurdles to therapeutics
2. Mystery to the origin of long-lived, skin-deep immune cells uncovered
3. Training cells to perform Boolean functions? Its logical
4. Heart Damage Repaired By Reprogramming Resident Fibroblasts into Functioning Heart Cells
5. New stem cell technique promises abundance of key heart cells
6. U of M study finds titan cells protect Cryptococcus
7. T cells hunt parasites like animal predators seek prey, a Penn Vet-Penn Physics study reveals
8. Math predicts size of clot-forming cells
9. New microscope uses rainbow of light to image the flow of individual blood cells
10. Growth factor in stem cells may spur recovery from MS
11. A cells first steps: Building a model to explain how cells grow
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Regenerated cells may restore vision after corneal dysfunction
(Date:1/3/2017)... , Jan. 3, 2017 Onitor, provider ... introduction of Onitor Track, an innovative biometric data-driven program ... showcasing this month at the 2017 Consumer Electronics Show ... In the U.S., the World Health Organization ... than two-thirds of adults who are overweight or obese. ...
(Date:12/20/2016)... RALEIGH, N.C. and GENEVA, Dec, 20, 2016 ... performance biometric data sensor technology, and STMicroelectronics ... across the spectrum of electronics applications, announced today ... scalable development kit for biometric wearables that includes ... integrated with Valencell,s Benchmark™ biometric sensor ...
(Date:12/16/2016)... 16, 2016 The global wearable medical device market, in ... 2021 from USD 5.31 billion in 2016, at a CAGR of ... ... technological advancements in medical devices, launch of a growing number of ... connectivity among healthcare providers, and increasing focus on physical fitness. ...
Breaking Biology News(10 mins):
(Date:1/24/2017)... GABLES, Fla. , Jan. 23, 2017 /PRNewswire-USNewswire/ ... network focused on scientific discovery and solutions, the ... creating the Frost Institutes for Science and Engineering ... engineering, and mathematics (STEM) to help solve some ... University of Miami President Julio Frenk ...
(Date:1/24/2017)...  Today, the Fisher Center for Alzheimer,s Research Foundation ... study that could lead to the development of effective ... This groundbreaking research was conducted by the Fisher Center ... Dr. Paul Greengard , in the quest to ... Center scientists have linked a mutation that protects elderly ...
(Date:1/23/2017)... 23, 2017  Spherix Incorporated (Nasdaq: SPEX ... fostering of technology and monetization of intellectual property, today ... Anthony Hayes , Chief Executive Officer ... continue to communicate with shareholders about the status of ... diligence on other patent assets that fit with our ...
(Date:1/23/2017)... Atlas Genetics Ltd., the ultra-rapid Point-Of-Care ... D financing, raising $35 million from a syndicate including all ... ... the Atlas Genetics io® system has been completed with the ... in February 2016.  This new Series D equity issue will ...
Breaking Biology Technology: