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Recognizing blood poisoning quickly
Date:12/9/2011

Is the patient suffering from blood poisoning? To answer this question, the doctor draws a blood sample and sends it to a central laboratory for testing. This takes up valuable time, which could cost the patient his life. In future, physicians will be able to analyze blood there and then and have the results within twenty minutes. This is made possible by a biochip, developed by scientists at the Fraunhofer Institute for Physical Measurement Techniques IPM in Freiburg. "To analyze the biochip we have also designed a fully automatic device to carry out all the examination steps," explains Dr. Albrecht Brandenburg, group manager at the IPM. "All the doctor has to do is place the sample in the apparatus and wait for the results."

Meanwhile, within the device there's plenty going on: it starts by preparing the blood sample. Red blood cells are separated from the blood and the plasma that remains is guided onto the biochip. When patients are suffering from sepsis, their immune system reacts by producing certain proteins. The biochip uses these in its diagnosis: there are antibodies positioned on the chip which fit these proteins like a key fits a lock. If the proteins are present in the blood, the antibodies fish them out of the fluid and bind them to the chip. But how does the apparatus know if proteins have been caught? "The chip is rinsed with a solution containing the appropriate antibodies, which have in turn been marked with a fluorescent dye," explains IPM scientist Dr. Manuel Kemmler. "These bind to the proteins meaning antibodies, protein and marked antibodies are all firmly linked to each other and to the chip's surface. When the chip is illuminated, the dye lights up." The apparatus sees lots of little illuminated dots that show the protein was in the blood. If the patient is healthy, however, the chip remains dark.

The researchers can even test for different proteins at the same time in one cycle. This is done by placing various differ
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Contact: Dr. Albrecht Brandenburg
albrecht.brandenburg@ipm.fraunhofer.de
49-761-885-7306
Fraunhofer-Gesellschaft
Source:Eurekalert  

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