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Recipe for capturing authentic embryonic stem cells may apply to any mammal, study suggests
Date:12/24/2008

t two decades, embryonic stem cells have been routinely used to create loss of function (knockout) or gene replacement (knockin) mutations by homologous recombination in the mouse, providing an invaluable tool for the functional characterization of genes," Ying's group wrote. "Now, the availability of true rat embryonic stem cells provides an opportunity to adapt the technology developed in the mouse to the rat."

The new findings raise "the possibility that culture formulations based on the 3i/2i principle could facilitate derivation of embryonic stem cells from other mammals, including livestock species," Austin Smith's team wrote. "It will also be of interest to investigate whether supernumerary human embryos cultured in 3i/2i may give rise to pluripotent cell lines that are qualitatively different from current human 'embryonic stem' cells" more like ground state rodent embryonic stem cells.


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Contact: Cathleen Genova
cgenova@cell.com
617-397-2802
Cell Press
Source:Eurekalert

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