Navigation Links
Rapid approach to identify influenza A virus mutations and drug resistance developed
Date:5/28/2009

Genome Institute of Singapore scientists, led by Christopher Wong, Ph.D., have developed a novel approach to uncover the complete sequence of any influenza A virus, including H1NI, with just a quick nasal swab or nasal pharyngeal wash from patients.

The new method, which enables scientists to amplify full genomes of influenza A viruses and sequence them within a day, was developed at the Genome Institute of Singapore (GIS), one of the research institutes sponsored by Singapore's A*STAR (Agency for Science, Development and Research), in collaboration with Roche NimbleGen.

The system, which combines a novel generic polymerase chain reaction (PCR) with NimbleGen's unique microarray-based platform, makes use of the same RNA (ribonucleic acid) material that is left over from traditional PCR-based diagnostics and is able to recognize any novel strain of influenza A in the first pass.

This enables a faster development of diagnostics for any possible new variant; it can also rapidly determine whether the strain has developed drug resistance. (Details of NimbleGen's microarray-based platform are provided below.)

"This new approach takes advantage of our novel PCR technology, developed for detecting a wide range of pathogens," said Dr. Wong, GIS Chief Scientific Officer for Biomarker Development. "This should greatly simplify the process of sequencing novel viruses."

The scientists' approach is also able to trace the mutations in the influenza A virus that may cause its resistance to drugs.

"The significance of this tracking process can be better appreciated in that it provides for vital information that can be used to prevent or combat a pandemic," said Edison Liu, M.D., GIS Executive Director.

This is especially important given the rapid spread of the new strain of the influenza A (nH1N1) virus. Experts have been concerned that the evolutionary track of this new strain might lead to mutation or re-assortment with other influenza strains with the potential to produce a more deadly strain, as the world experienced with the 1918 strain.

"With the development of this new system, the entire project team hopes to better and more quickly track this new flu variant and keep the world informed of how the virus is evolving," said Gerd Maass, Ph.D., CEO of Roche NimbleGen.

GIS scientists used NimbleGen arrays in a similar way during the SARS outbreak in 2003, to understand the infectious source and to globally monitor the SARS virus.

Roche NimbleGen's custom-developed microarrays:

The first arrays developed by Roche NimbleGen were designed, manufactured and shipped to Singapore just four days after the project was started. As the custom-developed high-density microarray contains probes which can reveal the complete sequence of the flu virus from patient samples, it enables the detection of any single base mutations in the regions of the genome, which is important for drug susceptibility. Where virus re-assortment has occurred, it will be able to identify which strain of influenza A it has recombined with, as well as the genomic location of the re-assortment to better understand and track the evolutionary path and variants of the virus.

NimbleGen's microarray-based customised platform is able to sequence as many as 36 genomes per day per chip reader device.


'/>"/>

Contact: Cathy Yarbrough
sciencematter@yahoo.com
858-243-1814
Agency for Science, Technology and Research (A*STAR), Singapore
Source:Eurekalert

Related biology news :

1. HudsonAlpha investigator develops rapid response swine flu test
2. Study finds hemlock trees dying rapidly, affecting forest carbon cycle
3. Scattered light rapidly detects tumor response to chemotherapy
4. Columbia University scientist devises new way to more rapidly generate bone tissue
5. Forsyth scientist receives major grant to support rapid, accurate, affordable test for tuberculosis
6. Reproducing early and often is the key to rapid evolution in plants
7. Rapid test for pathogens developed by K-State researchers
8. Video released of rapid Alzheimers improvement after new immune-based treatment
9. Surprisingly rapid changes in the Earths core discovered
10. Exploited fish make rapid comeback in worlds largest no-take marine reserve network
11. Birds migrate earlier, but some may be left behind as the climate warms rapidly
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/28/2016)... GOTHENBURG, Sweden , April 28, 2016 ... 1,491.2 M (139.9), up 966% compared with the first quarter of ... Operating profit totaled SEK 589.1 M (loss: 18.8) and the operating ... SEK 7.12 (loss: 0.32) Cash flow from operations was ... , The 2016 revenue guidance is unchanged, SEK 7,000-8,500 M. ...
(Date:4/15/2016)... 2016 Research and Markets has ... Market 2016-2020,"  report to their offering.  , ... ,The global gait biometrics market is expected to ... period 2016-2020. Gait analysis generates multiple ... used to compute factors that are not or ...
(Date:3/29/2016)... 2016 LegacyXChange, Inc. (OTC: ... SelectaDNA/CSI Protect are pleased to announce our successful effort ... variety of writing instruments, ensuring athletes signatures against counterfeiting ... from athletes on LegacyXChange will be assured of ongoing ... Bill Bollander , CEO states, "By ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... BOSTON , June 27, 2016   Ginkgo ... biology to industrial engineering, was today awarded as ... a selection of the world,s most innovative companies. ... at scale for the real world in the ... organism engineers work directly with customers including Fortune ...
(Date:6/24/2016)... ... June 24, 2016 , ... While the majority of commercial spectrophotometers and fluorometers ... the 6000i models are higher end machines that use the more unconventional z-dimension of ... beam from the bottom of the cuvette holder. , FireflySci has developed several ...
(Date:6/23/2016)... WI (PRWEB) , ... June 23, 2016 , ... ... supplements, is pleased to announce the launch of their brand, UP4™ Probiotics, into ... for over 35 years, is proud to add Target to its list of ...
(Date:6/23/2016)... SILVER SPRING, Md. , June 23, 2016 ... evidence collected from the crime scene to track the criminal ... sick, and the U.S. Food and Drug Administration (FDA) uses ... Sound far-fetched? It,s not. ... whole genome sequencing to support investigations of foodborne illnesses. Put ...
Breaking Biology Technology: