Navigation Links
Rapid and accurate mRNA detection in plant tissues
Date:4/17/2014

under study. Once the Z-probes bind to the mRNA molecules of interest, this allows label molecules to also bind, which then makes the mRNA molecules detectable, either via fluorescence or chromogenic detection.

According to Dr. Andrew Bowling, lead author of the paper and a research scientist at Dow Agrosciences, detected mRNA molecules appear as individual spots within the cell, making the expression results very easy to interpret.

"This method has a very low background level, which allows for higher confidence in the results obtained. With the conventional method, there seemed to always be a point where you were trying to determine if one tissue or cell was 'bluer' than another, or 'bluer' than the control. With the RNAscope method, the experimental sections have spots and the control sections don't. The results are very clear."

Bowling adds that RNAscope ISH is also a very accessible method compared to more traditional ISH approaches.

"The vendor provides probe design and synthesis services, greatly reducing the suite of skills that are required to carry out a successful ISH experiment. You don't have to be (or collaborate with) an RNA in vitro transcription expert to make your labeled probes. You don't have to synthesize plasmids to transcribe. You don't have to run northern blots to confirm efficacy and specificity. That is all part of the probe design and synthesis service. Also, it only takes about two weeks from emailing them a sequence to obtaining the probe in the mail from them."

"Furthermore, the [RNAScope ISH] method is faster. It takes about 810 hours to do an experiment. The classic ISH methods could sometimes require one or even two overnight incubations."


'/>"/>

Contact: Beth Parada
apps@botany.org
American Journal of Botany
Source:Eurekalert

Page: 1 2

Related biology news :

1. Low-cost, hydrogen-powered forklifts with rapid refueling, zero emissions coming soon
2. A non-invasive, rapid screening method for Alzheimers disease
3. 3D X-ray film: Rapid movements in real time
4. Rugged, rapid monitor safeguards space crews
5. Rapid climate changes, but with a 120 year time lag
6. Antarctic fjords are climate-sensitive hotspots of diversity in a rapidly warming region
7. mTOR: A key brain signaling mechanism for rapidly acting antidepressants
8. Researchers develop rapid, cost-effective early detection method for organ transplant injury
9. Insect soup serving up rapid biodiversity monitoring
10. U of M researchers unveil nations first porcine virus rapid detection test
11. Rapid upper ocean warming linked to declining aerosols
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/23/2014)... New Rochelle, NY -- The potential for clinical ... for transplant-based therapeutic strategies has previously been hindered ... the development of tumors. The ability to use ... for the treatment of heart disease, specifically acute ... Stem Cells and Development , a peer-reviewed ...
(Date:7/23/2014)... BCC Research ( ... MARKETS AND TECHNOLOGIES FOR SENSORS , the global market ... to nearly $154.4 billion by 2020, with a five-year ... flow, and level sensors segment is moving at a ... ) , Sensors have become indispensable to ...
(Date:7/23/2014)... . , Animals ... which might be very different from ours. Some bird ... sunlight in the atmosphere to calibrate their orientation systems. ... in Seewiesen, Germany, and Queen,s University Belfast have discovered ... the greater mouse-eared bat, has the capability to orient ...
Breaking Biology News(10 mins):Global Market for Sensors to Reach Nearly $154.4 Billion in 2020; Image Sensors Moving at 11.7% 2Global Market for Sensors to Reach Nearly $154.4 Billion in 2020; Image Sensors Moving at 11.7% 3Bats use the evening sky's polarization pattern for orientation 2
... has uncovered a protein that plays a vital role in ... in improving the production and quality of crops and biofuels. ... PNAS . The team included researchers from Dartmouth, ... Plant roots use their endodermis, or inner skin, as a ...
... Frederick will present new research at the 108th Annual ... and women,s beliefs about who should pay for dates ... expenses. The paper, Who Pays for Dates? Following versus ... than 17,000 participants; a quarter of whom also provided ...
... Vision licensee, Theresa Ayers , has added a second location ... Ronald Ayers , the husband-and-wife team assumed ownership last month ... Theresa Ayers has been with Pearle Vision, one of ... for more than three decades. She began her career working for ...
Cached Biology News:Research on which gender pays for dates shows change and resistance from convention 2Local Pearle Vision Licensee Adds Second Center In Canton, Ohio 2Local Pearle Vision Licensee Adds Second Center In Canton, Ohio 3Local Pearle Vision Licensee Adds Second Center In Canton, Ohio 4Local Pearle Vision Licensee Adds Second Center In Canton, Ohio 5
(Date:7/23/2014)... 23, 2014  Having the right people at the ... accelerating business growth and achieving clinical and operational goals. ... Leadership Summit, July 20-22, 2014, in San ... executives and healthcare experts discussing how partnerships have positively ... President & Chief Executive Officer of Cape Regional Medical ...
(Date:7/23/2014)... DES MOINES, Iowa (PRWEB) July 23, 2014 ... will join the business as vice president of Agricultural ... from Mendel Biotechnology where he served most recently as ... brings extensive biotechnology and business leadership in the seed ... DuPont Pioneer,” said Paul E. Schickler , president ...
(Date:7/23/2014)... 23, 2014  GrowBLOX Sciences, Inc. (OTCQB: GBLX), a medical ... filing for two medical marijuana dispensary locations in ... to the Clark County, Nevada dispensary ... as previously announced.  GrowBLOX Sciences, CEO Craig ... new applications for dispensaries in the City of ...
(Date:7/23/2014)... (PRWEB) July 23, 2014 OncLive® ... Cancer Center has joined its Strategic Alliance Partnership ... Alliance Partnership program, UNC Lineberger will have the ... raise awareness of its cutting-edge research initiatives, community-directed ... UNC Lineberger will work with OncLive to educate ...
Breaking Biology Technology:MSC and Cape Regional Medical Center Partner to Improve Cost, Quality and Outcomes 2DuPont Pioneer Names Neal Gutterson to Lead Agricultural Biotechnology 2DuPont Pioneer Names Neal Gutterson to Lead Agricultural Biotechnology 3GrowBLOX Sciences, Inc. Files for Two Additional Medical Marijuana Dispensaries in Las Vegas 2GrowBLOX Sciences, Inc. Files for Two Additional Medical Marijuana Dispensaries in Las Vegas 3OncLive Expands Its Strategic Alliance Partnership Program With the Addition of UNC Lineberger Comprehensive Cancer Center 2OncLive Expands Its Strategic Alliance Partnership Program With the Addition of UNC Lineberger Comprehensive Cancer Center 3
... , SAN FRANCISCO, Dec. 1 Medizone International, ... tests involving its proprietary AsepticSure(TM) technology, it continues to ... very different spore forming bacteria, Claustridium difficile and Bacillis ... potent sporicidal technology. "The implications of a 6.7 log ...
... ... comprehensive training programs, including biomedical repair technologies. Their shared efforts will begin in 2010 ... ... the recent signing of a Memorandum of Understanding, Mercy Ships and MediSend International have ...
... Cambridge, Mass November 30, 2009 An ... Photonics, and ETH Zrich have demonstrated compact, multibeam, ... of the light spectrum (infrared). By contrast, typical ... well-defined wavelength. The innovative multibeam lasers have potential ...
Cached Biology Technology:Medizone International Takes Aim at the Bioterrorism Countermeasures Arena 2Mercy Ships and MediSend International Partner to Deliver Hope and Healing 2Mercy Ships and MediSend International Partner to Deliver Hope and Healing 3Scientists demonstrate multibeam, multi-functional lasers 2
... dotLab System brings together two mature, ... immobilized capture surfaces. This combination produces ... for the detection of molecular binding ... labels. , Protein-specific capture molecules are ...
... polymers) are prepared by ... the enzyme, polynucleotide phosphorylase, ... the appropriate, ribonucleoside diphosphate ... exception of catalog number ...
... provides a comprehensive experiment based analysis package ... menus yet sophisticated and powerful functionality., Image ... CCD cameras for easy image capture. ... Digital Video Playback Saturation Warning ...
...
Biology Products: