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Rapid and accurate mRNA detection in plant tissues
Date:4/17/2014

under study. Once the Z-probes bind to the mRNA molecules of interest, this allows label molecules to also bind, which then makes the mRNA molecules detectable, either via fluorescence or chromogenic detection.

According to Dr. Andrew Bowling, lead author of the paper and a research scientist at Dow Agrosciences, detected mRNA molecules appear as individual spots within the cell, making the expression results very easy to interpret.

"This method has a very low background level, which allows for higher confidence in the results obtained. With the conventional method, there seemed to always be a point where you were trying to determine if one tissue or cell was 'bluer' than another, or 'bluer' than the control. With the RNAscope method, the experimental sections have spots and the control sections don't. The results are very clear."

Bowling adds that RNAscope ISH is also a very accessible method compared to more traditional ISH approaches.

"The vendor provides probe design and synthesis services, greatly reducing the suite of skills that are required to carry out a successful ISH experiment. You don't have to be (or collaborate with) an RNA in vitro transcription expert to make your labeled probes. You don't have to synthesize plasmids to transcribe. You don't have to run northern blots to confirm efficacy and specificity. That is all part of the probe design and synthesis service. Also, it only takes about two weeks from emailing them a sequence to obtaining the probe in the mail from them."

"Furthermore, the [RNAScope ISH] method is faster. It takes about 810 hours to do an experiment. The classic ISH methods could sometimes require one or even two overnight incubations."


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Contact: Beth Parada
apps@botany.org
American Journal of Botany
Source:Eurekalert

Page: 1 2

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