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Random picks better than complicated process in gene identification
Date:5/6/2009

relative levels of gene expression in a tissue sample."

Another key aspect of the paper is the novel use of analytical rarefaction in gene discovery.

In rarefaction, a species is selected at random from that ecosystem. Those selections are charted, with each selection considered one unit of effort. Once selections yield only previously selected species - or in this case, genes - the amount of effort needed to find all the species or genes has been determined. Scientists then know how much effort to use when searching for other genes.

"When it plateaus, you can give up. You've put in as much effort as you need," DeWoody said.

DeWoody and Hale tested the theory on samples from the reproductive organs of lake sturgeon while trying to find the genes responsible for determining fish sex. Hale said the work is important to conserve species by understanding their biological functions.

"Few studies have been done on threatened and endangered species. They're usually done on models such as mice and Arabidopsis," Hale said. "This species, the lake sturgeon, is a perfect example of a conservation concern."

The Great Lakes Fishery Trust and the Indiana Department of Natural Resources funded the research. DeWoody said the next step is to continue the process of finding the genes responsible for determining sex in sturgeon.


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Contact: Brian Wallheimer
bwallhei@purdue.edu
765-496-2050
Purdue University
Source:Eurekalert

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