Navigation Links
RNA-based methods for developmental studies are featured in Cold Spring Harbor Protocols
Date:2/1/2008

COLD SPRING HARBOR, N.Y. (Fri., Feb. 1, 2008) This months issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc2_08.dtl) highlights two methods to understand developmental processes in plants and flies. Both methods involve work with RNA and are freely accessible on the Web site for Cold Spring Harbor Protocols (www.cshprotocols.org).

The first protocol describes the use of RNAi to investigate gene function in fruit flies. Short RNA molecules are injected into fly embryos to disrupt the function of a specific gene. The embryos are then allowed to grow until they have reached a desired stage of development, when they are evaluated for changes that may have occurred due to the disruption of the gene.

The protocol, freely accessible at www.cshprotocols.org/cgi/content/full/2008/3/pdb.prot4918, is a recently updated version of one that originally appeared in the book Drosophila Protocols (http://www.cshlpress.com/link/drosprot.htm). It was developed by Dr. Bruce Patersons group at the National Institutes of Health (http://ccr.cancer.gov/staff/staff.asp?profileid=5613). His lab has used the protocol to investigate the regulation of gene expression during development, especially as it relates to muscle formation in the embryo.

The second protocol, available at www.cshprotocols.org/cgi/content/full/2008/3/pdb.prot4944, describes how to detect where and when a gene is expressed in young plant tissues such as embryos, seedlings, floral tissues, and other developing organs. The tissues are stained with a non-radioactive probe that specifically targets RNA molecules from a gene of interest. The staining pattern is then detected by microscopy. Scientists can analyze this staining pattern in a variety of tissue samples at different stages in development to gain a better understanding of the function of that gene. The method can be used to study virtually any plant species and will be useful to many biologists.


'/>"/>

Contact: Maria Smit
smit@cshl.edu
516-422-4127
Cold Spring Harbor Laboratory
Source:Eurekalert

Related biology news :

1. Alternative methods proposed to detect pesticides and antibiotics in water and natural food
2. Contraception: progress brings hope for new methods for men
3. From brains to behavior: Cold Spring Harbor Protocols features methods for neuroscience research
4. New book presents methods to poke and prod individual molecules
5. Old developmental pathways spawn revolutionary evolutionary changes
6. Researchers find association between food insecurity and developmental risk in children
7. Research pioneer in the developmental origins of psychiatric illness is awarded the Sackler prize
8. Claims of sex-related differences in genetic association studies often not properly validated
9. Genes, Environment and Health Initiative invests in genetic studies, environmental monitoring
10. NIH awards researcher $1.5 million new innovator grant for fruit-fly studies of prion proteins
11. Studies: Children obese due to a host of unhealthy pressures
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/4/2017)... -- For the thousands of attendees at this year,s International Consumer Electronics Show ... biometric measurement devices and services, will be featuring its new line of ... Medical,s special CES Exhibit Suite , the new upper arm and ... WellnessConnected product platform.  Continue ... ...
(Date:12/22/2016)... , Dec. 20, 2016  As part of its longstanding ... leading personal genetics company, recently released its latest children,s book, ... The book focuses on the topics of inheritance and variation ... Standards (NGSS) taught in elementary school classrooms in the US. ... series by illustrator Ariana Killoran , whose previous book ...
(Date:12/20/2016)... and GENEVA, Dec, 20, 2016   Valencell ... sensor technology, and STMicroelectronics (NYSE: STM), a ... of electronics applications, announced today the launch of ... for biometric wearables that includes ST,s compact ... Valencell,s Benchmark™ biometric sensor system. Together, SensorTile ...
Breaking Biology News(10 mins):
(Date:1/17/2017)... ... January 17, 2017 , ... ... Drug Safety Technology Consortium™ (SafeTEC™), $3 million in investment towards 15+ TEC Validation ... tools and assays, and their applicability in drug safety assessment, for the industry ...
(Date:1/16/2017)... NY (PRWEB) , ... January 16, 2017 , ... ... New Jersey laws precedential publication its decision on the appeal filed by India-based ... lawsuit of breach of contract against DPCL and one of its Dishman Group’s ...
(Date:1/14/2017)... , Jan. 13, 2017  The Alliance for ... in response to FDA final guidance on ... its continued leadership in emphasizing the importance of distinct ... keenly aware of the benefits biosimilars will bring to ... Yet the portion of the Guidance dealing ...
(Date:1/13/2017)... Island, NY (PRWEB) , ... January 13, 2017 ... ... teamed up with several companies to offer its customers three new solutions for ... probe would come in handy if a customer has an oddly-shaped sample that ...
Breaking Biology Technology: