Navigation Links
RNA-based methods for developmental studies are featured in Cold Spring Harbor Protocols
Date:2/1/2008

COLD SPRING HARBOR, N.Y. (Fri., Feb. 1, 2008) This months issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc2_08.dtl) highlights two methods to understand developmental processes in plants and flies. Both methods involve work with RNA and are freely accessible on the Web site for Cold Spring Harbor Protocols (www.cshprotocols.org).

The first protocol describes the use of RNAi to investigate gene function in fruit flies. Short RNA molecules are injected into fly embryos to disrupt the function of a specific gene. The embryos are then allowed to grow until they have reached a desired stage of development, when they are evaluated for changes that may have occurred due to the disruption of the gene.

The protocol, freely accessible at www.cshprotocols.org/cgi/content/full/2008/3/pdb.prot4918, is a recently updated version of one that originally appeared in the book Drosophila Protocols (http://www.cshlpress.com/link/drosprot.htm). It was developed by Dr. Bruce Patersons group at the National Institutes of Health (http://ccr.cancer.gov/staff/staff.asp?profileid=5613). His lab has used the protocol to investigate the regulation of gene expression during development, especially as it relates to muscle formation in the embryo.

The second protocol, available at www.cshprotocols.org/cgi/content/full/2008/3/pdb.prot4944, describes how to detect where and when a gene is expressed in young plant tissues such as embryos, seedlings, floral tissues, and other developing organs. The tissues are stained with a non-radioactive probe that specifically targets RNA molecules from a gene of interest. The staining pattern is then detected by microscopy. Scientists can analyze this staining pattern in a variety of tissue samples at different stages in development to gain a better understanding of the function of that gene. The method can be used to study virtually any plant species and will be useful to many biologists.


'/>"/>

Contact: Maria Smit
smit@cshl.edu
516-422-4127
Cold Spring Harbor Laboratory
Source:Eurekalert

Related biology news :

1. Alternative methods proposed to detect pesticides and antibiotics in water and natural food
2. Contraception: progress brings hope for new methods for men
3. From brains to behavior: Cold Spring Harbor Protocols features methods for neuroscience research
4. New book presents methods to poke and prod individual molecules
5. Old developmental pathways spawn revolutionary evolutionary changes
6. Researchers find association between food insecurity and developmental risk in children
7. Research pioneer in the developmental origins of psychiatric illness is awarded the Sackler prize
8. Claims of sex-related differences in genetic association studies often not properly validated
9. Genes, Environment and Health Initiative invests in genetic studies, environmental monitoring
10. NIH awards researcher $1.5 million new innovator grant for fruit-fly studies of prion proteins
11. Studies: Children obese due to a host of unhealthy pressures
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/16/2017)... , May 16, 2017  Veratad Technologies, LLC ... of online age and identity verification solutions, announced today ... Identity Conference 2017, May 15 thru May 17, 2017, ... Building and International Trade Center. Identity ... globe and in today,s quickly evolving digital world, defining ...
(Date:4/19/2017)... New York , April 19, 2017 ... competitive, as its vendor landscape is marked by the ... the market is however held by five major players ... Safran. Together these companies accounted for nearly 61% of ... of the leading companies in the global military biometrics ...
(Date:4/13/2017)... According to a new market research report "Consumer IAM Market ... Authorization), Service, Authentication Type, Deployment Mode, Vertical, and Region - Global Forecast ... from USD 14.30 Billion in 2017 to USD 31.75 Billion by 2022, ... ... MarketsandMarkets Logo ...
Breaking Biology News(10 mins):
(Date:5/23/2017)... ... May 23, 2017 , ... Customers often ... control technology again and again. METTLER TOLEDO has released two new videos that ... illustrate how integration of the ACT350 into Siemens and Allen Bradley PLCs is ...
(Date:5/22/2017)... Pittsburgh, PA (PRWEB) , ... May 22, 2017 ... ... Inc. announced today that it is exhibiting in booth B2 at the Association ... in Pittsburgh, May 22-25. , In addition to demonstrating its Cancer Diagnostic ...
(Date:5/19/2017)... Shirley, New York (PRWEB) , ... May 19, 2017 , ... ... therapy techniques in treating gait disorders, Biodex Medical Systems, Inc. announces the release of ... time music therapy has been joined with a biomedical system to aid in rehabilitating ...
(Date:5/18/2017)... ... May 18, 2017 , ... ... Tapas Cooking Challenge is a two-hour team-building package designed for groups of 10-30 ... Chef Jodi Abel, which include items, such as Blackened Shrimp with Edamame Salad, ...
Breaking Biology Technology: