Navigation Links
RNA-based methods for developmental studies are featured in Cold Spring Harbor Protocols
Date:2/1/2008

COLD SPRING HARBOR, N.Y. (Fri., Feb. 1, 2008) This months issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc2_08.dtl) highlights two methods to understand developmental processes in plants and flies. Both methods involve work with RNA and are freely accessible on the Web site for Cold Spring Harbor Protocols (www.cshprotocols.org).

The first protocol describes the use of RNAi to investigate gene function in fruit flies. Short RNA molecules are injected into fly embryos to disrupt the function of a specific gene. The embryos are then allowed to grow until they have reached a desired stage of development, when they are evaluated for changes that may have occurred due to the disruption of the gene.

The protocol, freely accessible at www.cshprotocols.org/cgi/content/full/2008/3/pdb.prot4918, is a recently updated version of one that originally appeared in the book Drosophila Protocols (http://www.cshlpress.com/link/drosprot.htm). It was developed by Dr. Bruce Patersons group at the National Institutes of Health (http://ccr.cancer.gov/staff/staff.asp?profileid=5613). His lab has used the protocol to investigate the regulation of gene expression during development, especially as it relates to muscle formation in the embryo.

The second protocol, available at www.cshprotocols.org/cgi/content/full/2008/3/pdb.prot4944, describes how to detect where and when a gene is expressed in young plant tissues such as embryos, seedlings, floral tissues, and other developing organs. The tissues are stained with a non-radioacti
'/>"/>

Contact: Maria Smit
smit@cshl.edu
516-422-4127
Cold Spring Harbor Laboratory
Source:Eurekalert

1

GOOD

Page: 1 2

Related biology news :

1. Alternative methods proposed to detect pesticides and antibiotics in water and natural food
2. Contraception: progress brings hope for new methods for men
3. From brains to behavior: Cold Spring Harbor Protocols features methods for neuroscience research
4. New book presents methods to poke and prod individual molecules
5. Old developmental pathways spawn revolutionary evolutionary changes
6. Researchers find association between food insecurity and developmental risk in children
7. Research pioneer in the developmental origins of psychiatric illness is awarded the Sackler prize
8. Claims of sex-related differences in genetic association studies often not properly validated
9. Genes, Environment and Health Initiative invests in genetic studies, environmental monitoring
10. NIH awards researcher $1.5 million new innovator grant for fruit-fly studies of prion proteins
11. Studies: Children obese due to a host of unhealthy pressures
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/25/2008)..., Arizona,s state soil, "Casa Grande," is featu...! The Secrets of Soil," at the Smithsonian,s Natur... will travel to museums across the country from 20... along with the President of the Soil Science Soci...cuss the new "Dig It! The Secrets of Soil" exhibit...
(Date:7/25/2008)..., Researchers, growers and Industry specialists f...nto the use of Brassica species, such as mustard...and weeds a technique known as biofumigation. ,...t suppress pests and pathogens, principally isothi...e as the ,hot, flavour in mustard or horseradish,"...
(Date:7/24/2008)..., A multi-institutional team led by Massachusetts...d a powerful new tool for genomic research and med...zymes that can target particular DNA sequences for... Molecular Cell, the researchers describe an eff...omized zinc-finger nucleases (ZFNs), which can be ...
(Date:7/24/2008)..., An infection due to a virus called cytomegalovi... compromised immune systems, can also affect hospi...tem problems, University of Washington researchers... longer hospital and intensive-care unit (ICU) sta...tudy, published July 23 in JAMA , the Journal of...
Breaking Biology News(10 mins):Consortium develops new method enabling routine targeted gene modification 2CMV infections affect more than just patients with compromised immune systems, researchers find 2Wyeth Announces Election of Timothy P Cost as Senior Vice President Corporate Affairs 3046 1Wyeth Announces Election of Timothy P Cost as Senior Vice President Corporate Affairs 3046 2Brain Injury May Not Erase Long Term Memory 11090 1Brain Injury May Not Erase Long Term Memory 11090 2Edwards Lifesciences Reports Strong Fourth Quarter Sales 11087 1Edwards Lifesciences Reports Strong Fourth Quarter Sales 11087 2Edwards Lifesciences Reports Strong Fourth Quarter Sales 11087 3Edwards Lifesciences Reports Strong Fourth Quarter Sales 11087 4Edwards Lifesciences Reports Strong Fourth Quarter Sales 11087 5Edwards Lifesciences Reports Strong Fourth Quarter Sales 11087 6Edwards Lifesciences Reports Strong Fourth Quarter Sales 11087 7Edwards Lifesciences Reports Strong Fourth Quarter Sales 11087 8Edwards Lifesciences Reports Strong Fourth Quarter Sales 11087 9Edwards Lifesciences Reports Strong Fourth Quarter Sales 11087 10Edwards Lifesciences Reports Strong Fourth Quarter Sales 11087 11Edwards Lifesciences Reports Strong Fourth Quarter Sales 11087 12Edwards Lifesciences Reports Strong Fourth Quarter Sales 11087 13Edwards Lifesciences Reports Strong Fourth Quarter Sales 11087 14Edwards Lifesciences Reports Strong Fourth Quarter Sales 11087 15Edwards Lifesciences Reports Strong Fourth Quarter Sales 11087 16Edwards Lifesciences Reports Strong Fourth Quarter Sales 11087 17California Department of Social Services Announces Improved Access to Disability Payments to Homeless Individuals 11083 1California Department of Social Services Announces Improved Access to Disability Payments to Homeless Individuals 11083 2
...ng multi-disciplinary analysis that included cogni...echniques, the team found both a low-level viral i...filtrated the brain in order to protect against th...ain immune cells achieve a level of control of the...ys Howard Fox, associate professor at Scripps Rese...
...ng multi-disciplinary analysis that included cogni...echniques, the team found both a low-level viral i...filtrated the brain in order to protect against th...ain immune cells achieve a level of control of the...ys Howard Fox, associate professor at Scripps Rese...
...expertise is being exported to North America to he... in as little as 30 years time. , Researchers fro..., working with The University of Arizona in the US... time will pinpoint the biggest threats to the rar...del are published in the academic journal Biologic...
... sweetgum tree grows widely throughout the country... are sometimes called "gumballs." Now, this spiny ... needed to make a lifesaving drug against bird flu...de, researchers say. , Chemists have found that th...amounts of shikimic acid, the starting material us...
Other Biology News:Experimental vaccine protects nonhuman primates when given after exposure to Marburg virus 2Immune response to HIV in the brain 2Computers to save unique type of American red squirrel 2Computers to save unique type of American red squirrel 3Sweetgum tree could help lessen shortage of bird flu drug 2
(Date:7/24/2008)...ows hospital physicians, pulmonary and critical ca... about overuse of antibiotics, DURHAM, N.C., July...two,surveys, fielded during conferences of the Ame...icine, that show how hospitalists, pulmonary and,c...mous in their view that,new methods and technologi...
(Date:7/24/2008)..., July 24 /- Glasses embedded with a ...h impaired vision to drive and do,other activities...esearch,Institute scientists describe the advantag...s in an article published in the May/June issue of...rs in print form this,month., "This new design ha...
(Date:7/24/2008)...TON, Mass., July 24 /- Instrumentatio...Inc., a leading supply chain services,company, has...ering its,critical care products and services. Bro...nts more than 915 acute care hospitals, more than ...00 physician practices., The contract covers IL,s...
(Date:7/24/2008)...RK, July 24 /- American Ori...ounced that it plans to release,second quarter 200...ter the,market closes., The Company will hold a c... discuss its fiscal second quarter 2008 results. L...9816 or 1-617-847-8705 for,international callers, ...
Breaking Biology Technology:U.S. Clinicians Indicate a Strong Need for New Tools to Combat Sepsis 2U.S. Clinicians Indicate a Strong Need for New Tools to Combat Sepsis 3Telescope Embedded in Spectacle Lens Promises to Make Driving Easier for Visually Impaired 2Telescope Embedded in Spectacle Lens Promises to Make Driving Easier for Visually Impaired 3Instrumentation Laboratory Announces Contract for Multi-Parameter Testing Products with Broadlane 2
...Stacy L. Deeds, and Edward J. Weinstein , ...een utilized in small to large scale screening pr... perform gene-silencing experiments in timeframes ...siRNA screens have become fairly common, large sca...ages of shRNA-based experiments include long-term...
...oduction , With the introduction of two... ODS-100V and TSKgel ODS-100Z, excellent chroma...an be achieved for a wide range of sample types. T...based on a spherical, five micron, silica gel , ...erivatized with difunctional , octadecylm...
...gley, Tosoh Bioscience LLC, Tatsunari Yoshida, Tos...tract: , The TSKgel Amide-80 column pro... peptides in hydrophilic interaction chromatogr...ties bonded to a silica base material. , ...ion of peptides with many acidic and basic residue...
...z Esser, multiBIND biotec GmbH, , ..., Prof. Dr. Thomas Lisowsky, multiBIND biote... Advanced experiments in gene technology demonstr...s,are sufficient to cause infections, recombinat...onally, the,complete decontamination of equipmen...
Other Biology Technology:A Screen of shRNAs Targeting Tumor Suppressor Genes to Identify Factors Involved in Paclitaxel Sensitivity 2A Screen of shRNAs Targeting Tumor Suppressor Genes to Identify Factors Involved in Paclitaxel Sensitivity 3A Screen of shRNAs Targeting Tumor Suppressor Genes to Identify Factors Involved in Paclitaxel Sensitivity 4A Screen of shRNAs Targeting Tumor Suppressor Genes to Identify Factors Involved in Paclitaxel Sensitivity 5A Screen of shRNAs Targeting Tumor Suppressor Genes to Identify Factors Involved in Paclitaxel Sensitivity 6Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins 2Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins 3Separation of Peptides by Hydrophilic Interaction Chromatography Using TSKgel Amide-80 2Separation of Peptides by Hydrophilic Interaction Chromatography Using TSKgel Amide-80 3Separation of Peptides by Hydrophilic Interaction Chromatography Using TSKgel Amide-80 4New Insights Into DNA Decontamination 2New Insights Into DNA Decontamination 3New Insights Into DNA Decontamination 4New Insights Into DNA Decontamination 5
Specificity: This antibody recognizes murine IRF7 protein, and does not cross-react with other related IRF proteins. Reactivity of this antibody with murine IRF7 protein was confirmed by western blot
GoTaq Flexi DNA Polymerase from Promega
EMBP (FL-222)
Rabbit anti-T-box 6
Biology Products: