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RD114 envelope proteins provide an effective and versatile approach to pseudotype lentiviral vectors
Date:9/30/2010

e envelopes: VSV-G, RD114 and RDpro with two lentiviral vectors. RDpro is an RD114-HIV chimera designed to pseudotype lentiviral vectors with higher efficiency. In the transient expression format, as expected VSV-G, provided the higher relative titer values. The nontoxic nature of the RD114, however, enabled typical transfection reactions to be extended from 48 to 96 hours. VSV-G transfections are generally limited to 48 hours. Hence, RD114 envelopes offer an effective means to increase the total viral yield of lentiviral supernatants vs. VSV-G. The versatility of RD114 proteins was exploited further to generate a "mixed" lentiviral expression system. This expression system is composed of stably expressed RD114 proteins that pseudotype lentiviral vectors generated in trans.

Dr. Bell said, "We are very excited about the data received in the mixed expression format. We were also pleasantly surprised that the native RD114 envelope outperformed the chimeric envelope, RDpro. In regard to gene therapy, we show that RDpro supernatants effectively transduce peripheral blood HSCs. We believe that this level of transduction can be enhanced further upon optimization of the concentration protocol."

Dr. Steven R. Goodman, Editor-in-Chief of Experimental Biology and Medicine said "This exciting study by Anthony Bell and colleagues demonstrates the value of using nontoxic envelope proteins as a viable alternative to pseudotype lentiviral vectors. This has great potential for future gene therapeutic approaches".


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Contact: Dr. Anthony J. Bell
Anthony.bell@usm.edu
601-266-5906
Society for Experimental Biology and Medicine
Source:Eurekalert

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