Navigation Links
Quick detection of periodontitis pathogens
Date:1/3/2013

Bleeding gums during tooth brushing or when biting into an apple could be an indication of periodontitis, an inflammatory disease of the tissues that surround and support the teeth. Bacterial plaque attacks the bone, meaning teeth can loosen over time and in the worst case even fall out, as they are left without a solid foundation to hold them in place. Furthermore, periodontitis also acts as a focal point from which disease can spread throughout the entire body: If the bacteria, which can be very aggressive, enter the bloodstream, they can cause further damage elsewhere. Physicians suspect there is a connection between periodontitis pathogens and the sort of cardiovascular damage that can cause heart attacks or strokes. In order to stop the source of inflammation, dentists remove dental calculus and deposits from the surface of teeth, but this is often not enough; particularly aggressive bacteria can only be eliminated with antibiotics.

Of the estimated 700 species of bacteria found in the mouth cavity, there are only eleven that are known to cause periodontal disease in particular; of these, some are deemed to be severely pathogenic. If these biomarkers are present in the gingival sulcus the small gap around the base of the tooth then the patient is at high risk of a severe form of periodontitis. But the only way to find out is by conducting a bacteria test. The problem is that current methods for identifying pathogens are time-consuming and must be carried out in an external contract laboratory. Conventional bacterial analysis using microbial culture carries the risk of bacteria being killed as soon as they come into contact with oxygen.

Bacterial analysis in less than 30 minutes

A new mobile diagnostic platform is designed to speed up identification of the eleven most relevant periodontitis pathogens considerably. Scientists at the Fraunhofer Institute for Cell Therapy and Immunology IZI in Leipzig have collaborated with two companies, BECIT GmbH and ERT-Optik, to develop a lab-on-a-chip module called ParoChip. In future this will allow dentists and medical labs to prepare samples quickly and then analyze the bacteria. All the steps in the process the duplication of DNA sequences and their detection take place directly on the platform, which consists of a disk-shaped microfluidic card that is around six centimeters in diameter. "Until now, analysis took around four to six hours. With ParoChip it takes less than 30 minutes. This means it's possible to analyze a large number of samples in a short amount of time," says Dr. Dirk Kuhlmeier, a scientist at the IZI.

The analysis is conducted in a contactless and fully automated manner. Samples are taken using sterile, toothpick-shaped paper points, after which the bacteria are removed from the point and their isolated DNA injected into reaction chambers containing dried reagents. There are eleven such chambers on each card, each featuring the reagent for one of the eleven periodontal pathogens. The total number of bacteria is determined in an additional chamber, via polymerase chain reaction (PCR). This method allows millions of copies of even tiny numbers of pathogen DNA sequences to be made. In order to generate the extremely quick changes in temperature that are required for PCR, the disk-shaped plastic chip is attached to a metal heating block with three temperature zones and mechanically turned so it passes over these zones. This causes a fluorescent signal to be generated that is measured by a connected optical measuring device featuring a fluorescence probe, a photo detector and a laser diode. The key benefit is that the signal makes it possible not only to quantify each type of bacterium and thus determine the severity of the inflammation, but also to establish the total number of all the bacteria combined. This enables doctors to fine-tune an antibiotic treatment accordingly.

"As the connected optical measuring system allows us to quantify bacteria, ParoChip is also suited to the identification of other bacterial causes of infection, such as food-borne pathogens or those that lead to sepsis ," says Kuhlmeier, who goes on to emphasize further advantages of the compact diagnostic platform: "Using ParoChip does away with many of the manual steps that are a necessary part of current bacteria tests. The synthetic disks can be produced cheaply and disposed of after use in the same way as disposable gloves." Already available as a prototype, ParoChip is initially intended for use in clinical laboratories; however it could also be used by dentists to carry out inhouse analysis of patient samples in their own practice.


'/>"/>

Contact: Dr. Dirk Kuhlmeier
dirk.kuhlmeier@izi.fraunhofer.de
49-341-355-369-312
Fraunhofer-Gesellschaft
Source:Eurekalert  

Related biology news :

1. Bitter tastes quickly turn milk chocolate fans sour
2. High doses of Vitamin D help tuberculosis patients recover more quickly
3. Global Information Inc. Announces Discounted Conference Registration For Bio-IT World Asia and Biodetection Technologies 2012
4. Early detection techniques offer hope for improved outcomes in lung cancer patients
5. Virus barcodes offer rapid detection of mutated strains
6. Earlier detection of bone loss may be in future
7. Diving board sensors key to DNA detection
8. Wayne State researchers working to improve genetic analysis, disorder detection
9. By detecting smallest virus, researchers open possibilities for early disease detection
10. Detection, analysis of cell dust may allow diagnosis, monitoring of brain cancer
11. Is the detection of early markers of Epstein Barr virus of diagnostic value?
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Quick detection of periodontitis pathogens
(Date:8/15/2017)... HAMPTON, Va. , Aug. 15 2017   ivWatch LLC ... effectiveness of intravenous (IV) therapy, today announced receipt of its ISO ... (QMS) developed by the International Organization for Standardization (ISO┬«). ... ivWatch Model 400 Continuous Monitoring device for ... "This ...
(Date:5/23/2017)... 2017  Hunova, the first robotic gym for the rehabilitation and functional ... in Genoa, Italy . The first 30 robots will ... USA . The technology was developed and patented at ... IIT spin-off Movendo Technology thanks to a 10 million euro investment from ... click: ...
(Date:4/19/2017)... New York , April 19, 2017 ... competitive, as its vendor landscape is marked by the ... the market is however held by five major players ... Safran. Together these companies accounted for nearly 61% of ... of the leading companies in the global military biometrics ...
Breaking Biology News(10 mins):
(Date:9/20/2017)... Palo Alto, CA (PRWEB) , ... September 20, ... ... and public interest organization focused on molecular manufacturing and other transformative technologies, announced ... categories, one for Experiment and the other for Theory in nanotechnology/molecular manufacturing. , ...
(Date:9/19/2017)... ... 19, 2017 , ... Participants of this educational webinar will ... Along with the advantages and disadvantages of ductless, filtered fume hoods, they will ... laboratory. , Attendees will learn from an industry expert about the different types ...
(Date:9/19/2017)... ... September 19, 2017 , ... The new and improved Oakton® pocket ... testers even stand upright with a new cap design that is versatile, functional and ... field who need to test water quality. , The Oakton pocket testers have many ...
(Date:9/19/2017)... (PRWEB) , ... September 19, 2017 , ... ... is pleased to announce the recipients of its 2017 Science Student Award. The ... leadership qualities, and involvement with community service defray the costs of obtaining their ...
Breaking Biology Technology: