Navigation Links
Quick, easy test identifies aggressive type of lung cancer in never-smokers
Date:2/25/2011

An inexpensive and rapid testing method can effectively identify a sub-group of never-smoking lung cancer patients whose tumors express a molecule associated with increased risk of disease progression or recurrence, US researchers have found.

Dr Ping Yang from the Mayo Clinic, Rochester, USA, and colleagues, reported the findings at the European Multidisciplinary Conference in Thoracic Oncology (EMCTO), 24-26 February 2011, Lugano, Switzerland.

Approximately 8% - 12% of patients with lung adenocarcinoma who have never smoked cigarettes carry tumors that express a protein product called anaplastic lymphoma kinase (ALK), Dr Yang said. "This subset of patients are at more than double the risk of experiencing disease progression or recurrence within 5 years of initial diagnosis compared to never-smokers whose lung adenocarcinoma tumors are ALK-negative."

The ability to test for ALK status in these patients may help doctors select the most appropriate therapies for maximum clinical benefit, Dr Yang explained. Her group's study aimed to see whether immunohistochemistry screening, followed by a confirmatory test using fluorescence in situ hybridization (FISH), is a practical way to do this.

Immunohistochemistry tests use antibodies to identify specific proteins in tissue sections; FISH uses fluorescent probes to bind to specific DNA sequences in the chromosomes of individual cells.

In a study of 303 samples, the research team found that immunohistochemistry results correlated well with the results of FISH. Tissue samples with the highest immunohistochemistry scores (IHC 3+) were all FISH positive, while those with no immunohistochemistry staining (IHC 0) were FISH negative.

Based on these findings, only samples that were scored with the intermediate IHC scores of 1+ and 2+ would need confirmatory testing with FISH, Dr Yang explained. "In this particular cohort, IHC score 2+ and 1+ cases represented approximately 27% of those screened. Even if confirmatory testing is done on all IHC 2+ and 1+ cases, confirmatory testing could be avoided on roughly three quarters of the cases."

Immunohistochemistry is routinely performed in pathology labs and is a relatively inexpensive and rapid test method. "Because it is already performed in most pathology labs, testing can be done on-site versus sending a sample to another diagnostic testing site. Thus, IHC may be a good initial screening tool for ALK status," Dr Yang explained.

Overall, the researchers found that 8.2-12.2% of this never-smoking group with lung adenocarcinoma had tumors that expressed ALK. The ALK positive tumors were significantly more aggressive as measured by tumor grade of differentiation and stage, and were diagnosed in younger patients, Dr Yang said. However, there are no guidelines or standard protocols for ALK testing by IHC at the present time; proper standardization and validation of IHC procedures for ALK testing are needed, including evaluating the effectiveness of different antibodies, Dr. Yang added.

Commenting on the research, which she was not involved in, Dr Fiona Blackhall, Consultant Medical Oncologist and Honorary Senior Lecturer at The Christie Hospital NHS Foundation Trust, and Manchester Cancer Research Centre, UK said: "Dr Yang and colleagues have conducted an important study showing that immunohistochemistry may be an efficient screen to identify cases for confirmatory FISH. Their work provides a basis from which to evolve a practical, clinical testing algorithm that incorporates IHC as a first step."

"Further work is now needed to validate these findings, optimize the methodology for IHC and the criteria for FISH," Dr Blackhall added. "Their finding that patients with ALK-positive tumors may have a worse outcome following surgery is provocative, highlighting the clinical and therapeutic importance of identifying and learning more about this molecular subtype of non-small cell lung cancer."


'/>"/>

Contact: Vanessa Pavinato
media@esmo.org
European Society for Medical Oncology
Source:Eurekalert

Related biology news :

1. Pitt-led researchers create quick, simple fluorescent detector for TB
2. Needle-in-a-haystack search identifies potential brain disease drug
3. UCSF study identifies chemicals in pregnant women
4. Study identifies new genetic signatures of breast cancer drug resistance
5. Research identifies drug target for prion diseases, mad cow
6. Large-scale analysis identifies new genetic alterations associated with height
7. Virginia Tech engineer identifies new concerns for antibiotic resistance, pollution
8. Transcription factor scan identifies genetic cause for inherited blindness
9. Motor Neurone Disease Association study identifies MND biomarker
10. New research identifies the plight of farmland birds
11. Study identifies key molecules in multiple myeloma
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/7/2016)... , June 7, 2016  Syngrafii Inc. ... a business relationship that includes integrating Syngrafii,s patented ... branch project. This collaboration will result in greater ... the credit union, while maintaining existing document workflow ... http://photos.prnewswire.com/prnh/20160606/375871LOGO ...
(Date:6/1/2016)... June 1, 2016 Favorable Government ... Administration and Criminal Identification to Boost Global Biometrics System ... released TechSci Research report, " Global Biometrics Market ... Forecast and Opportunities, 2011 - 2021", the global biometrics ... 2021, on account of growing security concerns across various ...
(Date:5/9/2016)... 2016 Elevay is currently known ... freedom for high net worth professionals seeking travel for ... connected world, there is still no substitute for a ... sealing your deal with a firm handshake. This is ... advantage of citizenship via investment programs like those offered ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... 23, 2016 /PRNewswire/ - FACIT has announced the ... biotechnology company, Propellon Therapeutics Inc. ("Propellon" or ... of a portfolio of first-in-class WDR5 inhibitors for ... as WDR5 represent an exciting class of therapies, ... medicine for cancer patients. Substantial advances have been ...
(Date:6/23/2016)... 2016  The Biodesign Challenge (BDC), a university competition ... harness living systems and biotechnology, announced its winning teams ... New York City . The ... projects at MoMA,s Celeste Bartos Theater during the daylong ... senior curator of architecture and design, and Suzanne ...
(Date:6/23/2016)... Ky. , June 23, 2016 ... two Phase 1 clinical trials of its complement ... placebo-controlled, single and multiple ascending dose studies designed ... pharmacodynamics (PD) of subcutaneous injection in healthy adult ... subcutaneously (SC) either as a single dose (ranging ...
(Date:6/23/2016)... ... June 23, 2016 , ... Regulatory Compliance Associates® Inc. (RCA), ... free webinar on Performing Quality Investigations: Getting to Root Cause. ... no charge. , Incomplete investigations are still a major concern to the Regulatory ...
Breaking Biology Technology: