Navigation Links
Putting light-harvesters on the spot
Date:10/19/2011

How the light-harvesting complexes required for photosynthesis get to their site of action in the plant cell is reported by RUB biologists in the Journal of Biological Chemistry. The team led by Prof. Dr. Danja Schnemann (RUB working group on the molecular biology of plant organelles) has demonstrated for the first time that a membrane protein interacts with a single soluble protein to anchor the subunits of the light-harvesting complexes in the membrane. The researchers propose a new model that explains the integration into the membrane through the formation of a pore.

Light harvesting

Photosynthesis occurs in special areas of the plant cells, the chloroplasts, whereby the energy-converting process takes place in specific protein complexes (photosystems). To capture the light energy and efficiently transmit it to the photosystems, light-harvesting complexes are required which work like antenna. "The proteins of the light-harvesting complexes are the most abundant membrane proteins on Earth" says Dr. Beatrix Dnschede of the RUB. "There is a special transport mechanism that conveys them into the chloroplasts and incorporates them into the photosynthetic membrane". Exactly how the various transport proteins interact with each other had, up to now, been unclear.

Interaction between only two proteins

Several soluble proteins and the membrane protein Alb3 that channels the proteins of the light-harvesting complexes into the membrane are involved in the transport. Bochum's biologists examined intact, isolated plant cells and found that, for this purpose, Alb3 interacts with only a single soluble transport protein (cpSRP43). They confirmed this result in a second experiment with artificial membrane systems. "In a further experiment, we identified the region in Alb3 to which the soluble protein cpSRP43 binds" explains the RUB biologist Dr. Thomas Bals. "It turned out that the binding site is partly within the membrane and thus cannot be freely accessible for cpSRP43."

Through the pore into the membrane

Schnemann's team explains the data with a new model. The soluble transport proteins bind the proteins of the light-harvesting complexes and transport them to the membrane. There, the soluble transport protein cpSRP43 interacts with the membrane protein Alb3, which then forms a pore. The proteins of the light-harvesting complexes get into the pore, and from there they are released laterally into the membrane. "There are proteins in other organisms which are very similar to Alb3 and apparently also form pores" says Dnschede. "This supports our model. We are now planning new experiments in order to recreate the entire transport path in an artificial system."


'/>"/>

Contact: Dr. Danja Schuenemann
danja.schuenemann@rub.de
49-234-322-4293
Ruhr-University Bochum
Source:Eurekalert

Related biology news :

1. Putting a price on sea fish
2. Putting trees on farms fundamental to future agricultural development
3. Putting the dead to work for conservation biology
4. Putting the dead to work
5. Putting a bulls-eye on the flu: Paper details influenzas structure for future drug targeting
6. Putting on the pounds after weight loss? Hit the gym to maintain health gains
7. By putting a ring on it, microparticles can be captured
8. Putting bacterial antibiotic resistance into reverse
9. Thrill-seeking holiday-makers are putting dolphins at risk
10. Thrill-seeking holidaymakers are putting dolphins at risk
11. Americans want Uncle Sams help putting healthy foods on their dinner table
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/16/2017)... 16, 2017  Veratad Technologies, LLC ( www.veratad.com ), ... and identity verification solutions, announced today they will participate ... May 15 thru May 17, 2017, in ... Trade Center. Identity impacts the lives ... today,s quickly evolving digital world, defining identity is critical ...
(Date:4/19/2017)... April 19, 2017 The global ... landscape is marked by the presence of several large ... held by five major players - 3M Cogent, NEC ... accounted for nearly 61% of the global military biometric ... in the global military biometrics market boast global presence, ...
(Date:4/13/2017)... , April 13, 2017 According to a new ... Authentication, Identity Analytics, Identity Administration, and Authorization), Service, Authentication Type, Deployment Mode, ... IAM Market is expected to grow from USD 14.30 Billion in 2017 ... (CAGR) of 17.3%. ... MarketsandMarkets Logo ...
Breaking Biology News(10 mins):
(Date:10/11/2017)... , ... October 11, 2017 , ... ... President Andi Purple announced Dr. Suneel I. Sheikh, the co-founder, CEO and ... Labs ), Inc. has been selected for membership in ARCS Alumni Hall ...
(Date:10/11/2017)... -- VMS BioMarketing, a leading provider of patient support solutions, has ... (CNE) network, which will launch this week. The VMS CNEs ... professionals to enhance the patient care experience by delivering peer-to-peer ... care professionals to help women who have been diagnosed and ... ...
(Date:10/10/2017)... ... October 10, 2017 , ... San Diego-based team building and ... rebranding initiative announced today. The bold new look is part of a transformation ... moves into a significant growth period. , It will also expand its service offering ...
(Date:10/10/2017)... 2017 SomaGenics announced the receipt of a ... RealSeq®-SC (Single Cell), expected to be the first commercially ... microRNAs) from single cells using NGS methods. The NIH,s ... accelerate development of approaches to analyze the heterogeneity of ... techniques for measuring levels of mRNAs in individual cells ...
Breaking Biology Technology: