Navigation Links
Putting bacterial antibiotic resistance into reverse
Date:4/25/2010

ANAHEIM, CA The use of antibiotics to treat bacterial infections causes a continual and vicious cycle in which antibiotic treatment leads to the emergence and spread of resistant strains, forcing the use of additional drugs leading to further multi-drug resistance.

But what if it doesn't have to be that way?

In a presentation at the American Society for Biochemistry and Molecular Biology's annual meeting, titled "Driving backwards the evolution of antibiotic resistance," Harvard researcher Roy Kishony will discuss his recent work showing that some drug combinations can stop or even reverse the normal trend, favoring bacteria that do not develop resistance. The talk will be in Anaheim Convention Center Room 304D, on Sunday April 25 at 3:30 pm PST.

"Normally, when clinicians administer a multi-drug regimen, they do so because the drugs act synergistically and speed up bacterial killing," Kishony explains. However, Kishony's laboratory has focused on the opposite phenomenon: antibiotic interactions that have a suppressive effect, namely when the combined inhibitory effect of using the two drugs together is weaker than that of one of the drugs alone.

Kishony and his team identified the suppressive interaction in E. coli, discovering that a combination of tetracycline which prevents bacteria from making proteins and ciprofloxacin which prevents them from copying their DNA was not as good as slowing down bacterial growth as one of the antibiotics (ciprofloxacin) by itself.

Kishony notes that this suppressive interaction can halt bacterial evolution, because any bacteria that develop a resistance to tetracycline will lose its suppressive effect against ciprofloxacin and die off; therefore, in a population the bacteria that remain non-resistant become the dominant strain.

While such a weakened antibiotic combination is not great from a clinical standpoint, the Kishony lab is using this discovery to set up a drug screening system that could identify novel drug combinations that could hinder the development of resistance but still act highly effectively. "Typical drug searches look for absolute killing effects, and choose the strongest candidates," he says. "Our approach is going to ask how these drugs affect the competition between resistant versus sensitive bacterial strains."

To develop such a screen, Kishony and his group first had to figure how this unusual interaction works.

"Fast growing bacteria like E. coli are optimized to balance their protein and DNA activity to grow and divide as quickly as the surrounding environment allows," Kishony explains. "However, when we exposed E. coli to the ciprofloxacin, we found that their optimization disappeared."

"We expected that since the bacteria would have more difficulty copying DNA, they would slow down their protein synthesis, too," Kishony continues. "But they didn't; they kept churning out proteins, which only added to their stress." However, once they added the tetracycline and protein synthesis was also reduced in the E. coli, they actually grew better than before. They then confirmed the idea that production of ribosomes - the cell components that make proteins - is too high under DNA stress by engineering E. coli strains that have fewer ribosomes than regular bacteria. While these mutants grew a more slowly in normal conditions, they grew faster under ciprofloxacin inhibition of DNA synthesis.

Kishony notes that their preliminary work on the development of a screen for drugs that put resistance in a disadvantage looks promising, and hopes that it would lead to the identification of novel drugs that select against resistance.


'/>"/>

Contact: Nicole Kresge
nkresge@asbmb.org
202-316-5447
Federation of American Societies for Experimental Biology
Source:Eurekalert

Related biology news :

1. Putting stem cell research on the fast track
2. Department of Energy putting power in the hands of consumers through technology
3. Putting a green cap on garbage dumps
4. Putting limits on vitamin E
5. Americans want Uncle Sams help putting healthy foods on their dinner table
6. Thrill-seeking holidaymakers are putting dolphins at risk
7. Thrill-seeking holiday-makers are putting dolphins at risk
8. Legionnaires bacterial proteins work together to survive
9. Scripps research team blocks bacterial communication system to prevent deadly staph infections
10. Small RNA plays parallel roles in bacterial metabolism
11. New drug targets may fight tuberculosis and other bacterial infections in novel way
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/5/2017)... , April 5, 2017 Today ... announcing that the server component of the HYPR platform ... for providing the end-to-end security architecture that empowers biometric ... HYPR has already secured over 15 million users ... including manufacturers of connected home product suites and physical ...
(Date:3/30/2017)... 2017  On April 6-7, 2017, Sequencing.com will host ... hackathon at Microsoft,s headquarters in Redmond, ... on developing health and wellness apps that provide a ... Genome is the first hackathon for personal genomics ... companies in the genomics, tech and health industries are ...
(Date:3/28/2017)... The report "Video Surveillance Market ... Storage Devices), Software (Video Analytics, VMS), and Service (VSaaS, ... to 2022", published by MarketsandMarkets, the market was valued ... to reach USD 75.64 Billion by 2022, at a ... year considered for the study is 2016 and the ...
Breaking Biology News(10 mins):
(Date:5/18/2017)... , ... May 18, 2017 , ... ... The Tapas Cooking Challenge is a two-hour team-building package designed for groups ... created by Chef Jodi Abel, which include items, such as Blackened Shrimp with ...
(Date:5/18/2017)... (PRWEB) , ... May 17, 2017 , ... ... specializing in medical device compliance and commercialization, has just released version 9.0 of ... work into this latest version of Cockpit,” says David Cronin, CEO of Cognition. ...
(Date:5/18/2017)... , ... May 17, 2017 , ... ... for example, are more likely to develop Alzheimer’s disease, while men are at ... account for this gender-specific neuronal bias is the aim of a research program ...
(Date:5/16/2017)... ... 16, 2017 , ... Zansors has secured a patent ... or EKG) acquisition and monitoring device. This Zansors’ next-generation intellectual property (IP), will ... the skin, making them significantly easier to deploy and use. , Currently, ECG ...
Breaking Biology Technology: